Preparation and Characterization of Diacetylene Polymerized Liposomes for Detection of Autoantibodies
Abstract Affinity diacetylene liposomes were prepared with 10,12-tricosadiynoic acid and cardiolipin as the affinity ligand for anticardiolipin antibodies at a molar ratio of 80:20. Polymerization was carried out under UV irradiation, and the color transitions were monitored by visible absorption sp...
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| Veröffentlicht in: | Journal of liposome research 2003-01, Vol.13 (3-4), p.199-211 |
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| container_title | Journal of liposome research |
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| creator | Cabral, E. C. M. Hennies, P. T. Correia, C. R. D. Zollner, R. L. Santana, M. H. A. |
| description | Abstract
Affinity diacetylene liposomes were prepared with 10,12-tricosadiynoic acid and cardiolipin as the affinity ligand for anticardiolipin antibodies at a molar ratio of 80:20. Polymerization was carried out under UV irradiation, and the color transitions were monitored by visible absorption spectroscopy. Peaks at 635 nm (blue form), 540 nm (purple form), and 480 nm (red form) were observed as a function of time. These polymerized liposomes were used in a noncompetitive immunoassay for detection of anticardiolipin antibodies. Color changes were observed when reference serum containing specific immunoglobulin G, IgG, was added to polymerized liposome dispersions. The colorimetric signal due to IgG adsorption on the liposome surface was quantified as a colorimetric response defined as the change in percentage of blue color related to the initial percentage of blue color in the solutions. The colorimetric response was 10 times higher for specific IgG compared with nonspecific ones. These results suggest the unique potentialities of affinity diacetylene polymerized liposomes in the development of biosensors for diagnosis of autoimmune diseases. |
| doi_str_mv | 10.1081/LPR-120026387 |
| format | Article |
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Affinity diacetylene liposomes were prepared with 10,12-tricosadiynoic acid and cardiolipin as the affinity ligand for anticardiolipin antibodies at a molar ratio of 80:20. Polymerization was carried out under UV irradiation, and the color transitions were monitored by visible absorption spectroscopy. Peaks at 635 nm (blue form), 540 nm (purple form), and 480 nm (red form) were observed as a function of time. These polymerized liposomes were used in a noncompetitive immunoassay for detection of anticardiolipin antibodies. Color changes were observed when reference serum containing specific immunoglobulin G, IgG, was added to polymerized liposome dispersions. The colorimetric signal due to IgG adsorption on the liposome surface was quantified as a colorimetric response defined as the change in percentage of blue color related to the initial percentage of blue color in the solutions. The colorimetric response was 10 times higher for specific IgG compared with nonspecific ones. These results suggest the unique potentialities of affinity diacetylene polymerized liposomes in the development of biosensors for diagnosis of autoimmune diseases.</description><identifier>ISSN: 0898-2104</identifier><identifier>EISSN: 1532-2394</identifier><identifier>DOI: 10.1081/LPR-120026387</identifier><identifier>PMID: 14670227</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>10,12-tricosadiynoic acid ; Acetylene - analogs & derivatives ; Acetylene - chemistry ; Anti-cardiolipin antibodies ; Autoantibodies - analysis ; Autoantibodies - immunology ; Cardiolipin ; Cardiolipins - chemistry ; Cardiolipins - immunology ; Fatty Acids, Unsaturated - chemistry ; Fatty Acids, Unsaturated - immunology ; Immunoassays ; Immunoglobulin G - analysis ; Immunoglobulin G - immunology ; Ligands ; Liposomes - chemical synthesis ; Liposomes - chemistry ; Liposomes - immunology ; Molecular Structure ; Polymerized liposomes ; Polymers - chemical synthesis ; Polymers - chemistry ; Spectrophotometry, Ultraviolet ; Time Factors</subject><ispartof>Journal of liposome research, 2003-01, Vol.13 (3-4), p.199-211</ispartof><rights>2003 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-d4675293f205c549eac392699c723dc285a049b8d50193fc623f9e435b9ed2d13</citedby><cites>FETCH-LOGICAL-c417t-d4675293f205c549eac392699c723dc285a049b8d50193fc623f9e435b9ed2d13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1081/LPR-120026387$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1081/LPR-120026387$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,59623,59729,60412,60518,61197,61232,61378,61413</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14670227$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cabral, E. C. M.</creatorcontrib><creatorcontrib>Hennies, P. T.</creatorcontrib><creatorcontrib>Correia, C. R. D.</creatorcontrib><creatorcontrib>Zollner, R. L.</creatorcontrib><creatorcontrib>Santana, M. H. A.</creatorcontrib><title>Preparation and Characterization of Diacetylene Polymerized Liposomes for Detection of Autoantibodies</title><title>Journal of liposome research</title><addtitle>J Liposome Res</addtitle><description>Abstract
Affinity diacetylene liposomes were prepared with 10,12-tricosadiynoic acid and cardiolipin as the affinity ligand for anticardiolipin antibodies at a molar ratio of 80:20. Polymerization was carried out under UV irradiation, and the color transitions were monitored by visible absorption spectroscopy. Peaks at 635 nm (blue form), 540 nm (purple form), and 480 nm (red form) were observed as a function of time. These polymerized liposomes were used in a noncompetitive immunoassay for detection of anticardiolipin antibodies. Color changes were observed when reference serum containing specific immunoglobulin G, IgG, was added to polymerized liposome dispersions. The colorimetric signal due to IgG adsorption on the liposome surface was quantified as a colorimetric response defined as the change in percentage of blue color related to the initial percentage of blue color in the solutions. The colorimetric response was 10 times higher for specific IgG compared with nonspecific ones. These results suggest the unique potentialities of affinity diacetylene polymerized liposomes in the development of biosensors for diagnosis of autoimmune diseases.</description><subject>10,12-tricosadiynoic acid</subject><subject>Acetylene - analogs & derivatives</subject><subject>Acetylene - chemistry</subject><subject>Anti-cardiolipin antibodies</subject><subject>Autoantibodies - analysis</subject><subject>Autoantibodies - immunology</subject><subject>Cardiolipin</subject><subject>Cardiolipins - chemistry</subject><subject>Cardiolipins - immunology</subject><subject>Fatty Acids, Unsaturated - chemistry</subject><subject>Fatty Acids, Unsaturated - immunology</subject><subject>Immunoassays</subject><subject>Immunoglobulin G - analysis</subject><subject>Immunoglobulin G - immunology</subject><subject>Ligands</subject><subject>Liposomes - chemical synthesis</subject><subject>Liposomes - chemistry</subject><subject>Liposomes - immunology</subject><subject>Molecular Structure</subject><subject>Polymerized liposomes</subject><subject>Polymers - chemical synthesis</subject><subject>Polymers - chemistry</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Time Factors</subject><issn>0898-2104</issn><issn>1532-2394</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1LKzEUxYMoWvtcvu1jVu5Gk5v5ylLq8wMKFvGtQ5rcoZGZSU0ySP3rX6RVcVFcXXLzu4fDOYT8ZvSC0YZdzhePOQNKoeJNfUAmrOSQAxfFIZnQRjQ5MFqckNMQnmmimhKOyQkrqpoC1BOCC49r5VW0bsjUYLLZKr10RG_ftkvXZtdWaYybDgfMFq7b9O-_aLK5XbvgegxZ63x2jRH1x8nVGJ0aol06YzH8Iket6gKe7eaU_Lv5-zS7y-cPt_ezq3muC1bH3CRbJQjeAi11WQhUmguohNA1cKOTeUULsWxMSVmidAW8FVjwcinQgGF8Ss63umvvXkYMUfY2aOw6NaAbg6xZUdasan4EmQCACiCB-RbU3oXgsZVrb3vlN5JR-V6ATAXIzwIS_2cnPC57NF_0LvEENFvADim0Xr063xkZ1aZzvvVq0DZIvk-7_na6QtXFlVYe5bMb_ZCS3ePqP5kfpgg</recordid><startdate>20030101</startdate><enddate>20030101</enddate><creator>Cabral, E. C. M.</creator><creator>Hennies, P. T.</creator><creator>Correia, C. R. D.</creator><creator>Zollner, R. L.</creator><creator>Santana, M. H. A.</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20030101</creationdate><title>Preparation and Characterization of Diacetylene Polymerized Liposomes for Detection of Autoantibodies</title><author>Cabral, E. C. M. ; Hennies, P. T. ; Correia, C. R. D. ; Zollner, R. L. ; Santana, M. H. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-d4675293f205c549eac392699c723dc285a049b8d50193fc623f9e435b9ed2d13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>10,12-tricosadiynoic acid</topic><topic>Acetylene - analogs & derivatives</topic><topic>Acetylene - chemistry</topic><topic>Anti-cardiolipin antibodies</topic><topic>Autoantibodies - analysis</topic><topic>Autoantibodies - immunology</topic><topic>Cardiolipin</topic><topic>Cardiolipins - chemistry</topic><topic>Cardiolipins - immunology</topic><topic>Fatty Acids, Unsaturated - chemistry</topic><topic>Fatty Acids, Unsaturated - immunology</topic><topic>Immunoassays</topic><topic>Immunoglobulin G - analysis</topic><topic>Immunoglobulin G - immunology</topic><topic>Ligands</topic><topic>Liposomes - chemical synthesis</topic><topic>Liposomes - chemistry</topic><topic>Liposomes - immunology</topic><topic>Molecular Structure</topic><topic>Polymerized liposomes</topic><topic>Polymers - chemical synthesis</topic><topic>Polymers - chemistry</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cabral, E. C. M.</creatorcontrib><creatorcontrib>Hennies, P. T.</creatorcontrib><creatorcontrib>Correia, C. R. D.</creatorcontrib><creatorcontrib>Zollner, R. L.</creatorcontrib><creatorcontrib>Santana, M. H. A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of liposome research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cabral, E. C. M.</au><au>Hennies, P. T.</au><au>Correia, C. R. D.</au><au>Zollner, R. L.</au><au>Santana, M. H. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation and Characterization of Diacetylene Polymerized Liposomes for Detection of Autoantibodies</atitle><jtitle>Journal of liposome research</jtitle><addtitle>J Liposome Res</addtitle><date>2003-01-01</date><risdate>2003</risdate><volume>13</volume><issue>3-4</issue><spage>199</spage><epage>211</epage><pages>199-211</pages><issn>0898-2104</issn><eissn>1532-2394</eissn><abstract>Abstract
Affinity diacetylene liposomes were prepared with 10,12-tricosadiynoic acid and cardiolipin as the affinity ligand for anticardiolipin antibodies at a molar ratio of 80:20. Polymerization was carried out under UV irradiation, and the color transitions were monitored by visible absorption spectroscopy. Peaks at 635 nm (blue form), 540 nm (purple form), and 480 nm (red form) were observed as a function of time. These polymerized liposomes were used in a noncompetitive immunoassay for detection of anticardiolipin antibodies. Color changes were observed when reference serum containing specific immunoglobulin G, IgG, was added to polymerized liposome dispersions. The colorimetric signal due to IgG adsorption on the liposome surface was quantified as a colorimetric response defined as the change in percentage of blue color related to the initial percentage of blue color in the solutions. The colorimetric response was 10 times higher for specific IgG compared with nonspecific ones. These results suggest the unique potentialities of affinity diacetylene polymerized liposomes in the development of biosensors for diagnosis of autoimmune diseases.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>14670227</pmid><doi>10.1081/LPR-120026387</doi><tpages>13</tpages></addata></record> |
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| source | MEDLINE; Taylor & Francis Medical Library - CRKN; Taylor & Francis Journals Complete |
| subjects | 10,12-tricosadiynoic acid Acetylene - analogs & derivatives Acetylene - chemistry Anti-cardiolipin antibodies Autoantibodies - analysis Autoantibodies - immunology Cardiolipin Cardiolipins - chemistry Cardiolipins - immunology Fatty Acids, Unsaturated - chemistry Fatty Acids, Unsaturated - immunology Immunoassays Immunoglobulin G - analysis Immunoglobulin G - immunology Ligands Liposomes - chemical synthesis Liposomes - chemistry Liposomes - immunology Molecular Structure Polymerized liposomes Polymers - chemical synthesis Polymers - chemistry Spectrophotometry, Ultraviolet Time Factors |
| title | Preparation and Characterization of Diacetylene Polymerized Liposomes for Detection of Autoantibodies |
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