Radiation-induced lipid peroxidation and membrane-bound enzymes in liver microsomes
Changes in lipid peroxidation and enzymes in liver microsomes were examined after alpha-irradiation. The radiation-induced lipid peroxidation was increased with increasing radiation dose, with decreasing dose-rate and also with decreasing concentration of microsomes. The content of P-450 was decreas...
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| Veröffentlicht in: | International journal of radiation biology 1980-06, Vol.37 (6), p.621-631 |
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| container_title | International journal of radiation biology |
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| creator | Yukawa, O Nakazawa, T |
| description | Changes in lipid peroxidation and enzymes in liver microsomes were examined after alpha-irradiation. The radiation-induced lipid peroxidation was increased with increasing radiation dose, with decreasing dose-rate and also with decreasing concentration of microsomes. The content of P-450 was decreased with decreasing dose-rate and inversely with radiation dose, while NADPH-cytochrome c reductase and cytochrome b5 were less affected by alpha-irradiation. Both the lipid peroxidation and the inactivation of microsomal enzymes induced by alpha-irradiation were mardedly reduced by radical scavengers. Hexobarbital hydroxylating activity and binding capacity of P-450 for hexobarbital, which were strikingly decreased after alpha-irradiation were less protected by radical scavengers, in contrast to the case of the lipid peroxidation and of the enzymes. These results suggest that intrinsic enzymes are more damaged through radiation-induced peroxidation of membrane lipids as compared with extrinsic enzymes. |
| doi_str_mv | 10.1080/713857573 |
| format | Article |
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The radiation-induced lipid peroxidation was increased with increasing radiation dose, with decreasing dose-rate and also with decreasing concentration of microsomes. The content of P-450 was decreased with decreasing dose-rate and inversely with radiation dose, while NADPH-cytochrome c reductase and cytochrome b5 were less affected by alpha-irradiation. Both the lipid peroxidation and the inactivation of microsomal enzymes induced by alpha-irradiation were mardedly reduced by radical scavengers. Hexobarbital hydroxylating activity and binding capacity of P-450 for hexobarbital, which were strikingly decreased after alpha-irradiation were less protected by radical scavengers, in contrast to the case of the lipid peroxidation and of the enzymes. These results suggest that intrinsic enzymes are more damaged through radiation-induced peroxidation of membrane lipids as compared with extrinsic enzymes.</description><identifier>ISSN: 0020-7616</identifier><identifier>ISSN: 0955-3002</identifier><identifier>DOI: 10.1080/713857573</identifier><identifier>PMID: 6968297</identifier><language>eng</language><publisher>England</publisher><subject>Animals ; Cell Membrane - enzymology ; Cell Membrane - radiation effects ; Cytochrome P-450 Enzyme System - metabolism ; Cytochrome P-450 Enzyme System - radiation effects ; Dose-Response Relationship, Radiation ; Free Radicals ; Gamma Rays ; Hexobarbital - pharmacology ; Lipid Peroxides - biosynthesis ; Lipid Peroxides - radiation effects ; Male ; Malondialdehyde - analysis ; Microsomes, Liver - enzymology ; Microsomes, Liver - radiation effects ; Rats</subject><ispartof>International journal of radiation biology, 1980-06, Vol.37 (6), p.621-631</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c278t-46e67c04bacfd34318f64271fa26ec6e7ee60feab6a43e024ff0f97bc939d77a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6968297$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yukawa, O</creatorcontrib><creatorcontrib>Nakazawa, T</creatorcontrib><title>Radiation-induced lipid peroxidation and membrane-bound enzymes in liver microsomes</title><title>International journal of radiation biology</title><addtitle>Int J Radiat Biol Relat Stud Phys Chem Med</addtitle><description>Changes in lipid peroxidation and enzymes in liver microsomes were examined after alpha-irradiation. The radiation-induced lipid peroxidation was increased with increasing radiation dose, with decreasing dose-rate and also with decreasing concentration of microsomes. The content of P-450 was decreased with decreasing dose-rate and inversely with radiation dose, while NADPH-cytochrome c reductase and cytochrome b5 were less affected by alpha-irradiation. Both the lipid peroxidation and the inactivation of microsomal enzymes induced by alpha-irradiation were mardedly reduced by radical scavengers. Hexobarbital hydroxylating activity and binding capacity of P-450 for hexobarbital, which were strikingly decreased after alpha-irradiation were less protected by radical scavengers, in contrast to the case of the lipid peroxidation and of the enzymes. These results suggest that intrinsic enzymes are more damaged through radiation-induced peroxidation of membrane lipids as compared with extrinsic enzymes.</description><subject>Animals</subject><subject>Cell Membrane - enzymology</subject><subject>Cell Membrane - radiation effects</subject><subject>Cytochrome P-450 Enzyme System - metabolism</subject><subject>Cytochrome P-450 Enzyme System - radiation effects</subject><subject>Dose-Response Relationship, Radiation</subject><subject>Free Radicals</subject><subject>Gamma Rays</subject><subject>Hexobarbital - pharmacology</subject><subject>Lipid Peroxides - biosynthesis</subject><subject>Lipid Peroxides - radiation effects</subject><subject>Male</subject><subject>Malondialdehyde - analysis</subject><subject>Microsomes, Liver - enzymology</subject><subject>Microsomes, Liver - radiation effects</subject><subject>Rats</subject><issn>0020-7616</issn><issn>0955-3002</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1LAzEQhnNQaq0e_AHCXj1EJx-b7B6l-AUFwY_zkk0mEGmyS7Yr1l_v1paehpn3mRl4CLlicMuggjvNRFXqUosTMgfgQLVi6oycD8MXQClrLmZkpmpV8VrPyfubccFsQpdoSG606Ip16IMreszdT3D_UWGSKyLGNpuEtO3GqcX0u404FCFNC9-Yixhs7oZuml2QU2_WA14e6oJ8Pj58LJ_p6vXpZXm_opbrakOlQqUtyNZY74QUrPJKcs284QqtQo2owKNplZECgUvvwde6tbWondZGLMjN_u7u8ZDRN30O0eRtw6DZuWiOLib2es_2YxvRHcmDCPEHdKxc5Q</recordid><startdate>198006</startdate><enddate>198006</enddate><creator>Yukawa, O</creator><creator>Nakazawa, T</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>198006</creationdate><title>Radiation-induced lipid peroxidation and membrane-bound enzymes in liver microsomes</title><author>Yukawa, O ; Nakazawa, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c278t-46e67c04bacfd34318f64271fa26ec6e7ee60feab6a43e024ff0f97bc939d77a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Animals</topic><topic>Cell Membrane - enzymology</topic><topic>Cell Membrane - radiation effects</topic><topic>Cytochrome P-450 Enzyme System - metabolism</topic><topic>Cytochrome P-450 Enzyme System - radiation effects</topic><topic>Dose-Response Relationship, Radiation</topic><topic>Free Radicals</topic><topic>Gamma Rays</topic><topic>Hexobarbital - pharmacology</topic><topic>Lipid Peroxides - biosynthesis</topic><topic>Lipid Peroxides - radiation effects</topic><topic>Male</topic><topic>Malondialdehyde - analysis</topic><topic>Microsomes, Liver - enzymology</topic><topic>Microsomes, Liver - radiation effects</topic><topic>Rats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yukawa, O</creatorcontrib><creatorcontrib>Nakazawa, T</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>International journal of radiation biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yukawa, O</au><au>Nakazawa, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Radiation-induced lipid peroxidation and membrane-bound enzymes in liver microsomes</atitle><jtitle>International journal of radiation biology</jtitle><addtitle>Int J Radiat Biol Relat Stud Phys Chem Med</addtitle><date>1980-06</date><risdate>1980</risdate><volume>37</volume><issue>6</issue><spage>621</spage><epage>631</epage><pages>621-631</pages><issn>0020-7616</issn><issn>0955-3002</issn><abstract>Changes in lipid peroxidation and enzymes in liver microsomes were examined after alpha-irradiation. The radiation-induced lipid peroxidation was increased with increasing radiation dose, with decreasing dose-rate and also with decreasing concentration of microsomes. The content of P-450 was decreased with decreasing dose-rate and inversely with radiation dose, while NADPH-cytochrome c reductase and cytochrome b5 were less affected by alpha-irradiation. Both the lipid peroxidation and the inactivation of microsomal enzymes induced by alpha-irradiation were mardedly reduced by radical scavengers. Hexobarbital hydroxylating activity and binding capacity of P-450 for hexobarbital, which were strikingly decreased after alpha-irradiation were less protected by radical scavengers, in contrast to the case of the lipid peroxidation and of the enzymes. These results suggest that intrinsic enzymes are more damaged through radiation-induced peroxidation of membrane lipids as compared with extrinsic enzymes.</abstract><cop>England</cop><pmid>6968297</pmid><doi>10.1080/713857573</doi><tpages>11</tpages></addata></record> |
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| ispartof | International journal of radiation biology, 1980-06, Vol.37 (6), p.621-631 |
| issn | 0020-7616 0955-3002 |
| language | eng |
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| source | MEDLINE; Taylor & Francis Medical Library - CRKN; Taylor & Francis Journals Complete |
| subjects | Animals Cell Membrane - enzymology Cell Membrane - radiation effects Cytochrome P-450 Enzyme System - metabolism Cytochrome P-450 Enzyme System - radiation effects Dose-Response Relationship, Radiation Free Radicals Gamma Rays Hexobarbital - pharmacology Lipid Peroxides - biosynthesis Lipid Peroxides - radiation effects Male Malondialdehyde - analysis Microsomes, Liver - enzymology Microsomes, Liver - radiation effects Rats |
| title | Radiation-induced lipid peroxidation and membrane-bound enzymes in liver microsomes |
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