Carboxymethylcellulose with phenolic hydroxyl microcapsules enclosinggene-modified BMSCs for controlled BMP-2 release in vitro
Objectives: The present study aimed to develop microparticles of phenolic hydroxyl derivative of carboxymethylcellulose (CMC-Ph) via Co-flow microfluidics technology and encapsulated gene-modified rat bone mesenchymal stem cells (BMSCs) for the detection of the growth factor release was controlled b...
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| Veröffentlicht in: | Artificial cells, nanomedicine, and biotechnology nanomedicine, and biotechnology, 2017-12, Vol.45 (8), p.1710-1720 |
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| creator | Du, Xiufan Huang, Fangli Zhang, Shujiang Yao, Yongchang Chen, Yi Chen, Yushu Huang, Hongxuan Bai, Bo |
| description | Objectives: The present study aimed to develop microparticles of phenolic hydroxyl derivative of carboxymethylcellulose (CMC-Ph) via Co-flow microfluidics technology and encapsulated gene-modified rat bone mesenchymal stem cells (BMSCs) for the detection of the growth factor release was controlled by Tet-on system. Meanwhile, we investigated the effect of the CMC-Ph microcapsules and Lentiviral transduction on osteogenesis of BMP2-BMSCs.
Methods: The middle size of CMC-Ph microcapsules was prepared by optimized co-flow microfluidics through ejecting fluid CMC-Ph suspension (mixed with HRP) into co-flowing liquid paraffin which blends H
2
O
2
at priority. The Lentivirus-encoding hBMP-2 and Tet-On system were constructed and amplified by RT-PCR, then encapsulated in the microcapsules. The cellular viability of CMC-Ph microparticles was assessed by Live/dead staining and metabolic activity was estimated by colorimetric assay kit. In addition, BMP-2 secretion and kinetic studies were determined by ELISA, alkaline phosphatase (ALP) activity was evaluated using ALP assay kit, and ALP staining as well as mineral calcium deposition was detected by alizarin red S staining.
Key findings: The diameter of CMC-Ph microparticles was controlled between 100 and 150 μm by altering the flow speed of liquid paraffin and then encapsulated bone morphogenetic protein 2 (BMP-2) gene modified BMSCs transduced by a lentiviral vector. Moreover, the mitochondrial activity of the encapsulated cells was maintained at least 24 d and BMP-2 protein secretion into the supernatant sustained for 35 d without significant loss of efficiency under the induction of the doxycycline. Furthermore, mineral deposition staining and ALP activity detection showed that encapsulated lentiviral-BMP2 transduced BMSCs possess more osteogenic differentiation potential than normal cells.
Conclusions: Co-flow microfluidics and phenolic hydroxyl derivative of carboxymethylcellulose (CMC-Ph) provide a promising strategy for cell-enclosed microcapsules in combination with BMP-2 gene and Tet-on system modified BMSCs and then controlled BMP-2 protein released effectively as well as promoted the osteogenic differentiation of BMSCs. |
| doi_str_mv | 10.1080/21691401.2017.1282499 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1080_21691401_2017_1282499</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1862760880</sourcerecordid><originalsourceid>FETCH-LOGICAL-c394t-9ffe328fe8b18826f88198749c6adac1d1f983469a7cc132787ce07a1194eb283</originalsourceid><addsrcrecordid>eNp9kU1v1DAQhiNERau2PwFkiQuXLB4nG9s3YAW0UqsiARI3y-uMu64ce7GTllz47XjZbQ8c8MXW-Jl3Pt6qegl0AVTQtww6CS2FBaPAF8AEa6V8Vp3s4jW08OP505vCcXWe8x0tR0DHl-2L6pgJYLKT3Un1e6XTOv6aBxw3szfo_eRjRvLgxg3ZbjBE7wzZzH0qkCeDMykavc2Tx0wwmAK7cHuLAesh9s467MmH66-rTGxMxMQwpuj93-CXmpGEHnWRd4Hcu_J1Vh1Z7TOeH-7T6vunj99WF_XVzefL1fur2jSyHWtpLTZMWBRrEIJ1VgiQgrfSdLrXBnqwUjRtJzU3BhrGBTdIuQaQLa6ZaE6rN3vdbYo_J8yjGlzeTasDxikrEB3jHRWCFvT1P-hdnFIo3SmQS87okgso1HJPlX3knNCqbXKDTrMCqnYeqUeP1M4jdfCo5L06qE_rAfunrEdHCvBuD7hQNjjoh5h8r0Y9-5hs0sG4rJr_1_gDG-WhpQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1957205781</pqid></control><display><type>article</type><title>Carboxymethylcellulose with phenolic hydroxyl microcapsules enclosinggene-modified BMSCs for controlled BMP-2 release in vitro</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Du, Xiufan ; Huang, Fangli ; Zhang, Shujiang ; Yao, Yongchang ; Chen, Yi ; Chen, Yushu ; Huang, Hongxuan ; Bai, Bo</creator><creatorcontrib>Du, Xiufan ; Huang, Fangli ; Zhang, Shujiang ; Yao, Yongchang ; Chen, Yi ; Chen, Yushu ; Huang, Hongxuan ; Bai, Bo</creatorcontrib><description>Objectives: The present study aimed to develop microparticles of phenolic hydroxyl derivative of carboxymethylcellulose (CMC-Ph) via Co-flow microfluidics technology and encapsulated gene-modified rat bone mesenchymal stem cells (BMSCs) for the detection of the growth factor release was controlled by Tet-on system. Meanwhile, we investigated the effect of the CMC-Ph microcapsules and Lentiviral transduction on osteogenesis of BMP2-BMSCs.
Methods: The middle size of CMC-Ph microcapsules was prepared by optimized co-flow microfluidics through ejecting fluid CMC-Ph suspension (mixed with HRP) into co-flowing liquid paraffin which blends H
2
O
2
at priority. The Lentivirus-encoding hBMP-2 and Tet-On system were constructed and amplified by RT-PCR, then encapsulated in the microcapsules. The cellular viability of CMC-Ph microparticles was assessed by Live/dead staining and metabolic activity was estimated by colorimetric assay kit. In addition, BMP-2 secretion and kinetic studies were determined by ELISA, alkaline phosphatase (ALP) activity was evaluated using ALP assay kit, and ALP staining as well as mineral calcium deposition was detected by alizarin red S staining.
Key findings: The diameter of CMC-Ph microparticles was controlled between 100 and 150 μm by altering the flow speed of liquid paraffin and then encapsulated bone morphogenetic protein 2 (BMP-2) gene modified BMSCs transduced by a lentiviral vector. Moreover, the mitochondrial activity of the encapsulated cells was maintained at least 24 d and BMP-2 protein secretion into the supernatant sustained for 35 d without significant loss of efficiency under the induction of the doxycycline. Furthermore, mineral deposition staining and ALP activity detection showed that encapsulated lentiviral-BMP2 transduced BMSCs possess more osteogenic differentiation potential than normal cells.
Conclusions: Co-flow microfluidics and phenolic hydroxyl derivative of carboxymethylcellulose (CMC-Ph) provide a promising strategy for cell-enclosed microcapsules in combination with BMP-2 gene and Tet-on system modified BMSCs and then controlled BMP-2 protein released effectively as well as promoted the osteogenic differentiation of BMSCs.</description><identifier>ISSN: 2169-1401</identifier><identifier>EISSN: 2169-141X</identifier><identifier>DOI: 10.1080/21691401.2017.1282499</identifier><identifier>PMID: 28129696</identifier><language>eng</language><publisher>England: Taylor & Francis</publisher><subject>Alizarin ; Alkaline phosphatase ; Alkaline Phosphatase - metabolism ; Animals ; Biocompatibility ; Biomedical materials ; BMP-2 ; Bone morphogenetic protein 2 ; Bone Morphogenetic Protein 2 - secretion ; Calcium ; Calcium - metabolism ; Capsules ; Carboxymethylcellulose ; Carboxymethylcellulose Sodium - chemistry ; Cell Differentiation - genetics ; Cell Survival ; Colorimetry ; Deposition ; Differentiation ; Doxycycline ; Ejection ; Enzyme-linked immunosorbent assay ; Fluid flow ; Hydrogen peroxide ; lentiviral ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - metabolism ; Mesenchymal Stromal Cells - secretion ; Mesenchyme ; Microcapsules ; Microencapsulation ; Microfluidics ; Microparticles ; Mitochondria ; Mixtures ; Osteogenesis ; Osteogenesis - genetics ; osteogenic differentiation ; Paraffin ; Phenolic compounds ; Phenolic hydroxyl derivative of carboxymethylcellulose (CMC-Ph) ; Phenols - chemistry ; Polymerase chain reaction ; Proteins ; Rats ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Secretion ; Staining ; Stem cell transplantation ; Stem cells ; Tet-on system ; Transduction, Genetic</subject><ispartof>Artificial cells, nanomedicine, and biotechnology, 2017-12, Vol.45 (8), p.1710-1720</ispartof><rights>2017 Informa UK Limited, trading as Taylor & Francis Group 2017</rights><rights>2017 Informa UK Limited, trading as Taylor & Francis Group</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c394t-9ffe328fe8b18826f88198749c6adac1d1f983469a7cc132787ce07a1194eb283</citedby><cites>FETCH-LOGICAL-c394t-9ffe328fe8b18826f88198749c6adac1d1f983469a7cc132787ce07a1194eb283</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28129696$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Du, Xiufan</creatorcontrib><creatorcontrib>Huang, Fangli</creatorcontrib><creatorcontrib>Zhang, Shujiang</creatorcontrib><creatorcontrib>Yao, Yongchang</creatorcontrib><creatorcontrib>Chen, Yi</creatorcontrib><creatorcontrib>Chen, Yushu</creatorcontrib><creatorcontrib>Huang, Hongxuan</creatorcontrib><creatorcontrib>Bai, Bo</creatorcontrib><title>Carboxymethylcellulose with phenolic hydroxyl microcapsules enclosinggene-modified BMSCs for controlled BMP-2 release in vitro</title><title>Artificial cells, nanomedicine, and biotechnology</title><addtitle>Artif Cells Nanomed Biotechnol</addtitle><description>Objectives: The present study aimed to develop microparticles of phenolic hydroxyl derivative of carboxymethylcellulose (CMC-Ph) via Co-flow microfluidics technology and encapsulated gene-modified rat bone mesenchymal stem cells (BMSCs) for the detection of the growth factor release was controlled by Tet-on system. Meanwhile, we investigated the effect of the CMC-Ph microcapsules and Lentiviral transduction on osteogenesis of BMP2-BMSCs.
Methods: The middle size of CMC-Ph microcapsules was prepared by optimized co-flow microfluidics through ejecting fluid CMC-Ph suspension (mixed with HRP) into co-flowing liquid paraffin which blends H
2
O
2
at priority. The Lentivirus-encoding hBMP-2 and Tet-On system were constructed and amplified by RT-PCR, then encapsulated in the microcapsules. The cellular viability of CMC-Ph microparticles was assessed by Live/dead staining and metabolic activity was estimated by colorimetric assay kit. In addition, BMP-2 secretion and kinetic studies were determined by ELISA, alkaline phosphatase (ALP) activity was evaluated using ALP assay kit, and ALP staining as well as mineral calcium deposition was detected by alizarin red S staining.
Key findings: The diameter of CMC-Ph microparticles was controlled between 100 and 150 μm by altering the flow speed of liquid paraffin and then encapsulated bone morphogenetic protein 2 (BMP-2) gene modified BMSCs transduced by a lentiviral vector. Moreover, the mitochondrial activity of the encapsulated cells was maintained at least 24 d and BMP-2 protein secretion into the supernatant sustained for 35 d without significant loss of efficiency under the induction of the doxycycline. Furthermore, mineral deposition staining and ALP activity detection showed that encapsulated lentiviral-BMP2 transduced BMSCs possess more osteogenic differentiation potential than normal cells.
Conclusions: Co-flow microfluidics and phenolic hydroxyl derivative of carboxymethylcellulose (CMC-Ph) provide a promising strategy for cell-enclosed microcapsules in combination with BMP-2 gene and Tet-on system modified BMSCs and then controlled BMP-2 protein released effectively as well as promoted the osteogenic differentiation of BMSCs.</description><subject>Alizarin</subject><subject>Alkaline phosphatase</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Animals</subject><subject>Biocompatibility</subject><subject>Biomedical materials</subject><subject>BMP-2</subject><subject>Bone morphogenetic protein 2</subject><subject>Bone Morphogenetic Protein 2 - secretion</subject><subject>Calcium</subject><subject>Calcium - metabolism</subject><subject>Capsules</subject><subject>Carboxymethylcellulose</subject><subject>Carboxymethylcellulose Sodium - chemistry</subject><subject>Cell Differentiation - genetics</subject><subject>Cell Survival</subject><subject>Colorimetry</subject><subject>Deposition</subject><subject>Differentiation</subject><subject>Doxycycline</subject><subject>Ejection</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Fluid flow</subject><subject>Hydrogen peroxide</subject><subject>lentiviral</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>Mesenchymal Stromal Cells - secretion</subject><subject>Mesenchyme</subject><subject>Microcapsules</subject><subject>Microencapsulation</subject><subject>Microfluidics</subject><subject>Microparticles</subject><subject>Mitochondria</subject><subject>Mixtures</subject><subject>Osteogenesis</subject><subject>Osteogenesis - genetics</subject><subject>osteogenic differentiation</subject><subject>Paraffin</subject><subject>Phenolic compounds</subject><subject>Phenolic hydroxyl derivative of carboxymethylcellulose (CMC-Ph)</subject><subject>Phenols - chemistry</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>Rats</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Secretion</subject><subject>Staining</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Tet-on system</subject><subject>Transduction, Genetic</subject><issn>2169-1401</issn><issn>2169-141X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1v1DAQhiNERau2PwFkiQuXLB4nG9s3YAW0UqsiARI3y-uMu64ce7GTllz47XjZbQ8c8MXW-Jl3Pt6qegl0AVTQtww6CS2FBaPAF8AEa6V8Vp3s4jW08OP505vCcXWe8x0tR0DHl-2L6pgJYLKT3Un1e6XTOv6aBxw3szfo_eRjRvLgxg3ZbjBE7wzZzH0qkCeDMykavc2Tx0wwmAK7cHuLAesh9s467MmH66-rTGxMxMQwpuj93-CXmpGEHnWRd4Hcu_J1Vh1Z7TOeH-7T6vunj99WF_XVzefL1fur2jSyHWtpLTZMWBRrEIJ1VgiQgrfSdLrXBnqwUjRtJzU3BhrGBTdIuQaQLa6ZaE6rN3vdbYo_J8yjGlzeTasDxikrEB3jHRWCFvT1P-hdnFIo3SmQS87okgso1HJPlX3knNCqbXKDTrMCqnYeqUeP1M4jdfCo5L06qE_rAfunrEdHCvBuD7hQNjjoh5h8r0Y9-5hs0sG4rJr_1_gDG-WhpQ</recordid><startdate>201712</startdate><enddate>201712</enddate><creator>Du, Xiufan</creator><creator>Huang, Fangli</creator><creator>Zhang, Shujiang</creator><creator>Yao, Yongchang</creator><creator>Chen, Yi</creator><creator>Chen, Yushu</creator><creator>Huang, Hongxuan</creator><creator>Bai, Bo</creator><general>Taylor & Francis</general><general>Taylor & Francis Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201712</creationdate><title>Carboxymethylcellulose with phenolic hydroxyl microcapsules enclosinggene-modified BMSCs for controlled BMP-2 release in vitro</title><author>Du, Xiufan ; Huang, Fangli ; Zhang, Shujiang ; Yao, Yongchang ; Chen, Yi ; Chen, Yushu ; Huang, Hongxuan ; Bai, Bo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c394t-9ffe328fe8b18826f88198749c6adac1d1f983469a7cc132787ce07a1194eb283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Alizarin</topic><topic>Alkaline phosphatase</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>Animals</topic><topic>Biocompatibility</topic><topic>Biomedical materials</topic><topic>BMP-2</topic><topic>Bone morphogenetic protein 2</topic><topic>Bone Morphogenetic Protein 2 - secretion</topic><topic>Calcium</topic><topic>Calcium - metabolism</topic><topic>Capsules</topic><topic>Carboxymethylcellulose</topic><topic>Carboxymethylcellulose Sodium - chemistry</topic><topic>Cell Differentiation - genetics</topic><topic>Cell Survival</topic><topic>Colorimetry</topic><topic>Deposition</topic><topic>Differentiation</topic><topic>Doxycycline</topic><topic>Ejection</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Fluid flow</topic><topic>Hydrogen peroxide</topic><topic>lentiviral</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - metabolism</topic><topic>Mesenchymal Stromal Cells - secretion</topic><topic>Mesenchyme</topic><topic>Microcapsules</topic><topic>Microencapsulation</topic><topic>Microfluidics</topic><topic>Microparticles</topic><topic>Mitochondria</topic><topic>Mixtures</topic><topic>Osteogenesis</topic><topic>Osteogenesis - genetics</topic><topic>osteogenic differentiation</topic><topic>Paraffin</topic><topic>Phenolic compounds</topic><topic>Phenolic hydroxyl derivative of carboxymethylcellulose (CMC-Ph)</topic><topic>Phenols - chemistry</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>Rats</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Secretion</topic><topic>Staining</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Tet-on system</topic><topic>Transduction, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Du, Xiufan</creatorcontrib><creatorcontrib>Huang, Fangli</creatorcontrib><creatorcontrib>Zhang, Shujiang</creatorcontrib><creatorcontrib>Yao, Yongchang</creatorcontrib><creatorcontrib>Chen, Yi</creatorcontrib><creatorcontrib>Chen, Yushu</creatorcontrib><creatorcontrib>Huang, Hongxuan</creatorcontrib><creatorcontrib>Bai, Bo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Artificial cells, nanomedicine, and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Du, Xiufan</au><au>Huang, Fangli</au><au>Zhang, Shujiang</au><au>Yao, Yongchang</au><au>Chen, Yi</au><au>Chen, Yushu</au><au>Huang, Hongxuan</au><au>Bai, Bo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Carboxymethylcellulose with phenolic hydroxyl microcapsules enclosinggene-modified BMSCs for controlled BMP-2 release in vitro</atitle><jtitle>Artificial cells, nanomedicine, and biotechnology</jtitle><addtitle>Artif Cells Nanomed Biotechnol</addtitle><date>2017-12</date><risdate>2017</risdate><volume>45</volume><issue>8</issue><spage>1710</spage><epage>1720</epage><pages>1710-1720</pages><issn>2169-1401</issn><eissn>2169-141X</eissn><abstract>Objectives: The present study aimed to develop microparticles of phenolic hydroxyl derivative of carboxymethylcellulose (CMC-Ph) via Co-flow microfluidics technology and encapsulated gene-modified rat bone mesenchymal stem cells (BMSCs) for the detection of the growth factor release was controlled by Tet-on system. Meanwhile, we investigated the effect of the CMC-Ph microcapsules and Lentiviral transduction on osteogenesis of BMP2-BMSCs.
Methods: The middle size of CMC-Ph microcapsules was prepared by optimized co-flow microfluidics through ejecting fluid CMC-Ph suspension (mixed with HRP) into co-flowing liquid paraffin which blends H
2
O
2
at priority. The Lentivirus-encoding hBMP-2 and Tet-On system were constructed and amplified by RT-PCR, then encapsulated in the microcapsules. The cellular viability of CMC-Ph microparticles was assessed by Live/dead staining and metabolic activity was estimated by colorimetric assay kit. In addition, BMP-2 secretion and kinetic studies were determined by ELISA, alkaline phosphatase (ALP) activity was evaluated using ALP assay kit, and ALP staining as well as mineral calcium deposition was detected by alizarin red S staining.
Key findings: The diameter of CMC-Ph microparticles was controlled between 100 and 150 μm by altering the flow speed of liquid paraffin and then encapsulated bone morphogenetic protein 2 (BMP-2) gene modified BMSCs transduced by a lentiviral vector. Moreover, the mitochondrial activity of the encapsulated cells was maintained at least 24 d and BMP-2 protein secretion into the supernatant sustained for 35 d without significant loss of efficiency under the induction of the doxycycline. Furthermore, mineral deposition staining and ALP activity detection showed that encapsulated lentiviral-BMP2 transduced BMSCs possess more osteogenic differentiation potential than normal cells.
Conclusions: Co-flow microfluidics and phenolic hydroxyl derivative of carboxymethylcellulose (CMC-Ph) provide a promising strategy for cell-enclosed microcapsules in combination with BMP-2 gene and Tet-on system modified BMSCs and then controlled BMP-2 protein released effectively as well as promoted the osteogenic differentiation of BMSCs.</abstract><cop>England</cop><pub>Taylor & Francis</pub><pmid>28129696</pmid><doi>10.1080/21691401.2017.1282499</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2169-1401 |
| ispartof | Artificial cells, nanomedicine, and biotechnology, 2017-12, Vol.45 (8), p.1710-1720 |
| issn | 2169-1401 2169-141X |
| language | eng |
| recordid | cdi_crossref_primary_10_1080_21691401_2017_1282499 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Alizarin Alkaline phosphatase Alkaline Phosphatase - metabolism Animals Biocompatibility Biomedical materials BMP-2 Bone morphogenetic protein 2 Bone Morphogenetic Protein 2 - secretion Calcium Calcium - metabolism Capsules Carboxymethylcellulose Carboxymethylcellulose Sodium - chemistry Cell Differentiation - genetics Cell Survival Colorimetry Deposition Differentiation Doxycycline Ejection Enzyme-linked immunosorbent assay Fluid flow Hydrogen peroxide lentiviral Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - metabolism Mesenchymal Stromal Cells - secretion Mesenchyme Microcapsules Microencapsulation Microfluidics Microparticles Mitochondria Mixtures Osteogenesis Osteogenesis - genetics osteogenic differentiation Paraffin Phenolic compounds Phenolic hydroxyl derivative of carboxymethylcellulose (CMC-Ph) Phenols - chemistry Polymerase chain reaction Proteins Rats RNA, Messenger - genetics RNA, Messenger - metabolism Secretion Staining Stem cell transplantation Stem cells Tet-on system Transduction, Genetic |
| title | Carboxymethylcellulose with phenolic hydroxyl microcapsules enclosinggene-modified BMSCs for controlled BMP-2 release in vitro |
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