Establishment and characterization of organoids from a patient with adenomyoepithelioma of the breast
Adenomyoepithelioma (AME) of the breast is a rare tumor that is composed of proliferating epithelial and myoepithelial cells. The pathogenesis of AME remains unclear, and no breast cancer cells have been identified in such tumor tissues. In this study, we established patient-derived breast cancer or...
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| Veröffentlicht in: | Bioengineered 2021-12, Vol.12 (2), p.11578-11585 |
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| container_title | Bioengineered |
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| creator | Luo, XiangRong She, JianTao Xu, Tao Zhou, Yuan Xu, ChuanBo Jiang, JianPing Li, TianGang Liu, Huajiang Shen, Hui Yin, Bolong Dai, Bin |
| description | Adenomyoepithelioma (AME) of the breast is a rare tumor that is composed of proliferating epithelial and myoepithelial cells. The pathogenesis of AME remains unclear, and no breast cancer cells have been identified in such tumor tissues. In this study, we established patient-derived breast cancer organoids from the surgical tumor samples of an elderly Chinese woman with an AME of the breast. Our findings confirmed the successful establishment of organoids from an AME of the breast of this patient. A short tandem repeat analysis revealed that the DNA signature of the AME of the breast organoids matched the DNA signature of the original tumor specimen. Moreover, diameter assay confirmed that the organoids from the breast AME showed sensitivity to paclitaxel and doxorubicin treatments, which was similar to, but lesser than that of primary culture cells. In conclusion, we established an efficient 3-dimensional breast cancer organoid culture platform from an AME of the breast. This platform can be effectively used for exploring clinicopathological and genomic characteristics of AME of the breast to identify possible treatments and increase awareness about this disease entity. |
| doi_str_mv | 10.1080/21655979.2021.1974809 |
| format | Article |
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The pathogenesis of AME remains unclear, and no breast cancer cells have been identified in such tumor tissues. In this study, we established patient-derived breast cancer organoids from the surgical tumor samples of an elderly Chinese woman with an AME of the breast. Our findings confirmed the successful establishment of organoids from an AME of the breast of this patient. A short tandem repeat analysis revealed that the DNA signature of the AME of the breast organoids matched the DNA signature of the original tumor specimen. Moreover, diameter assay confirmed that the organoids from the breast AME showed sensitivity to paclitaxel and doxorubicin treatments, which was similar to, but lesser than that of primary culture cells. In conclusion, we established an efficient 3-dimensional breast cancer organoid culture platform from an AME of the breast. This platform can be effectively used for exploring clinicopathological and genomic characteristics of AME of the breast to identify possible treatments and increase awareness about this disease entity.</description><identifier>ISSN: 2165-5979</identifier><identifier>EISSN: 2165-5987</identifier><identifier>DOI: 10.1080/21655979.2021.1974809</identifier><identifier>PMID: 34874791</identifier><language>eng</language><publisher>United States: Taylor & Francis</publisher><subject>3-d breast cancer organoids ; Adenomyoepithelioma - diagnostic imaging ; Adenomyoepithelioma - drug therapy ; Adenomyoepithelioma - genetics ; Adenomyoepithelioma - pathology ; Adenomyoepithelioma of the breast ; Aged ; breast cancer ; Breast Neoplasms - diagnostic imaging ; Breast Neoplasms - drug therapy ; Breast Neoplasms - genetics ; Breast Neoplasms - pathology ; drug sensitivity test ; Female ; Humans ; Microsatellite Repeats - genetics ; organoid culture ; Organoids - pathology ; Research Paper ; Tumor Cells, Cultured</subject><ispartof>Bioengineered, 2021-12, Vol.12 (2), p.11578-11585</ispartof><rights>2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. 2021</rights><rights>2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. 2021 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c468t-7bb4b764379eb4e1e74105a65bd2db53327a910e93ea1c6b731216556c9137683</citedby><cites>FETCH-LOGICAL-c468t-7bb4b764379eb4e1e74105a65bd2db53327a910e93ea1c6b731216556c9137683</cites><orcidid>0000-0003-0149-3233</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810105/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810105/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27502,27924,27925,53791,53793,59143,59144</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34874791$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Luo, XiangRong</creatorcontrib><creatorcontrib>She, JianTao</creatorcontrib><creatorcontrib>Xu, Tao</creatorcontrib><creatorcontrib>Zhou, Yuan</creatorcontrib><creatorcontrib>Xu, ChuanBo</creatorcontrib><creatorcontrib>Jiang, JianPing</creatorcontrib><creatorcontrib>Li, TianGang</creatorcontrib><creatorcontrib>Liu, Huajiang</creatorcontrib><creatorcontrib>Shen, Hui</creatorcontrib><creatorcontrib>Yin, Bolong</creatorcontrib><creatorcontrib>Dai, Bin</creatorcontrib><title>Establishment and characterization of organoids from a patient with adenomyoepithelioma of the breast</title><title>Bioengineered</title><addtitle>Bioengineered</addtitle><description>Adenomyoepithelioma (AME) of the breast is a rare tumor that is composed of proliferating epithelial and myoepithelial cells. The pathogenesis of AME remains unclear, and no breast cancer cells have been identified in such tumor tissues. In this study, we established patient-derived breast cancer organoids from the surgical tumor samples of an elderly Chinese woman with an AME of the breast. Our findings confirmed the successful establishment of organoids from an AME of the breast of this patient. A short tandem repeat analysis revealed that the DNA signature of the AME of the breast organoids matched the DNA signature of the original tumor specimen. Moreover, diameter assay confirmed that the organoids from the breast AME showed sensitivity to paclitaxel and doxorubicin treatments, which was similar to, but lesser than that of primary culture cells. In conclusion, we established an efficient 3-dimensional breast cancer organoid culture platform from an AME of the breast. This platform can be effectively used for exploring clinicopathological and genomic characteristics of AME of the breast to identify possible treatments and increase awareness about this disease entity.</description><subject>3-d breast cancer organoids</subject><subject>Adenomyoepithelioma - diagnostic imaging</subject><subject>Adenomyoepithelioma - drug therapy</subject><subject>Adenomyoepithelioma - genetics</subject><subject>Adenomyoepithelioma - pathology</subject><subject>Adenomyoepithelioma of the breast</subject><subject>Aged</subject><subject>breast cancer</subject><subject>Breast Neoplasms - diagnostic imaging</subject><subject>Breast Neoplasms - drug therapy</subject><subject>Breast Neoplasms - genetics</subject><subject>Breast Neoplasms - pathology</subject><subject>drug sensitivity test</subject><subject>Female</subject><subject>Humans</subject><subject>Microsatellite Repeats - genetics</subject><subject>organoid culture</subject><subject>Organoids - pathology</subject><subject>Research Paper</subject><subject>Tumor Cells, Cultured</subject><issn>2165-5979</issn><issn>2165-5987</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>EIF</sourceid><recordid>eNp9UctuFDEQHEWgJAr5hCAfuexij98XBIrCQ4rEhZyttseTNZqxF9tLtHw9HnazggunflVVt7q67obgNcEKv-2J4FxLve5xT9ZES6awPusul_6KayVfnHKpL7rrUr5jjAmmjEt13l1QpiSTmlx2_q5UsFMom9nHiiAOyG0gg6s-h19QQ4oojSjlR4gpDAWNOc0I0LaNFsJTqBsEg49p3ie_bZWfQpphIbUc2eyh1FfdyxGm4q-P8ap7-Hj37fbz6v7rpy-3H-5XjglVV9JaZqVgVGpvmSdeMoI5CG6HfrCc0l6CJthr6oE4YSUlfz4hnCZUCkWvuncH3e3Ozn5w7cIMk9nmMEPemwTB_DuJYWMe00-jFGnv4U3gzVEgpx87X6qZQ3F-miD6tCumF1gR2hMsGpQfoC6nUrIfT2sINotL5tkls7hkji413uu_bzyxnj1pgPcHQIhjyjM8pTwNpsJ-SnnMEF0ohv5_x2-K06NO</recordid><startdate>20211220</startdate><enddate>20211220</enddate><creator>Luo, XiangRong</creator><creator>She, JianTao</creator><creator>Xu, Tao</creator><creator>Zhou, Yuan</creator><creator>Xu, ChuanBo</creator><creator>Jiang, JianPing</creator><creator>Li, TianGang</creator><creator>Liu, Huajiang</creator><creator>Shen, Hui</creator><creator>Yin, Bolong</creator><creator>Dai, Bin</creator><general>Taylor & Francis</general><scope>0YH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-0149-3233</orcidid></search><sort><creationdate>20211220</creationdate><title>Establishment and characterization of organoids from a patient with adenomyoepithelioma of the breast</title><author>Luo, XiangRong ; She, JianTao ; Xu, Tao ; Zhou, Yuan ; Xu, ChuanBo ; Jiang, JianPing ; Li, TianGang ; Liu, Huajiang ; Shen, Hui ; Yin, Bolong ; Dai, Bin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c468t-7bb4b764379eb4e1e74105a65bd2db53327a910e93ea1c6b731216556c9137683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>3-d breast cancer organoids</topic><topic>Adenomyoepithelioma - diagnostic imaging</topic><topic>Adenomyoepithelioma - drug therapy</topic><topic>Adenomyoepithelioma - genetics</topic><topic>Adenomyoepithelioma - pathology</topic><topic>Adenomyoepithelioma of the breast</topic><topic>Aged</topic><topic>breast cancer</topic><topic>Breast Neoplasms - diagnostic imaging</topic><topic>Breast Neoplasms - drug therapy</topic><topic>Breast Neoplasms - genetics</topic><topic>Breast Neoplasms - pathology</topic><topic>drug sensitivity test</topic><topic>Female</topic><topic>Humans</topic><topic>Microsatellite Repeats - genetics</topic><topic>organoid culture</topic><topic>Organoids - pathology</topic><topic>Research Paper</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Luo, XiangRong</creatorcontrib><creatorcontrib>She, JianTao</creatorcontrib><creatorcontrib>Xu, Tao</creatorcontrib><creatorcontrib>Zhou, Yuan</creatorcontrib><creatorcontrib>Xu, ChuanBo</creatorcontrib><creatorcontrib>Jiang, JianPing</creatorcontrib><creatorcontrib>Li, TianGang</creatorcontrib><creatorcontrib>Liu, Huajiang</creatorcontrib><creatorcontrib>Shen, Hui</creatorcontrib><creatorcontrib>Yin, Bolong</creatorcontrib><creatorcontrib>Dai, Bin</creatorcontrib><collection>Access via Taylor & Francis (Open Access Collection)</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Bioengineered</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Luo, XiangRong</au><au>She, JianTao</au><au>Xu, Tao</au><au>Zhou, Yuan</au><au>Xu, ChuanBo</au><au>Jiang, JianPing</au><au>Li, TianGang</au><au>Liu, Huajiang</au><au>Shen, Hui</au><au>Yin, Bolong</au><au>Dai, Bin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment and characterization of organoids from a patient with adenomyoepithelioma of the breast</atitle><jtitle>Bioengineered</jtitle><addtitle>Bioengineered</addtitle><date>2021-12-20</date><risdate>2021</risdate><volume>12</volume><issue>2</issue><spage>11578</spage><epage>11585</epage><pages>11578-11585</pages><issn>2165-5979</issn><eissn>2165-5987</eissn><abstract>Adenomyoepithelioma (AME) of the breast is a rare tumor that is composed of proliferating epithelial and myoepithelial cells. The pathogenesis of AME remains unclear, and no breast cancer cells have been identified in such tumor tissues. In this study, we established patient-derived breast cancer organoids from the surgical tumor samples of an elderly Chinese woman with an AME of the breast. Our findings confirmed the successful establishment of organoids from an AME of the breast of this patient. A short tandem repeat analysis revealed that the DNA signature of the AME of the breast organoids matched the DNA signature of the original tumor specimen. Moreover, diameter assay confirmed that the organoids from the breast AME showed sensitivity to paclitaxel and doxorubicin treatments, which was similar to, but lesser than that of primary culture cells. In conclusion, we established an efficient 3-dimensional breast cancer organoid culture platform from an AME of the breast. This platform can be effectively used for exploring clinicopathological and genomic characteristics of AME of the breast to identify possible treatments and increase awareness about this disease entity.</abstract><cop>United States</cop><pub>Taylor & Francis</pub><pmid>34874791</pmid><doi>10.1080/21655979.2021.1974809</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-0149-3233</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 3-d breast cancer organoids Adenomyoepithelioma - diagnostic imaging Adenomyoepithelioma - drug therapy Adenomyoepithelioma - genetics Adenomyoepithelioma - pathology Adenomyoepithelioma of the breast Aged breast cancer Breast Neoplasms - diagnostic imaging Breast Neoplasms - drug therapy Breast Neoplasms - genetics Breast Neoplasms - pathology drug sensitivity test Female Humans Microsatellite Repeats - genetics organoid culture Organoids - pathology Research Paper Tumor Cells, Cultured |
| title | Establishment and characterization of organoids from a patient with adenomyoepithelioma of the breast |
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