Nitric oxide suppresses the inhibitory effect of abscisic acid on seed germination by S-nitrosylation of SnRK2 proteins
Nitric oxide (NO) plays important roles in plant development, and biotic and abiotic stress responses. In a recent study, we showed that endogenous NO negatively regulates abscisic acid (ABA) signaling in guard cells by inhibiting sucrose nonfermenting 1 (SNF1)-related protein kinase 2.6 (SnRK2.6)/o...
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| Veröffentlicht in: | Plant signaling & behavior 2015-06, Vol.10 (6), p.e1031939-e1031939 |
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| creator | Wang, Pengcheng Zhu, Jian-Kang Lang, Zhaobo |
| description | Nitric oxide (NO) plays important roles in plant development, and biotic and abiotic stress responses. In a recent study, we showed that endogenous NO negatively regulates abscisic acid (ABA) signaling in guard cells by inhibiting sucrose nonfermenting 1 (SNF1)-related protein kinase 2.6 (SnRK2.6)/open stomata 1(OST1) through S-nitrosylation. Application of NO breaks seed dormancy and alleviates the inhibitory effect of ABA on seed germination and early seedling growth, but it is unclear how NO functions at the stages of seed germination and early seedling development. Here, we show that like SnRK2.6, SnRK2.2 can be inactivated by S-nitrosoglutathione (GSNO) treatment through S-nitrosylation. SnRK2.2 and the closely related SnRK2.3 are known to play redundant roles in ABA inhibition of seed germination in Arabidopsis. We found that treatment with the NO donor SNP phenocopies the snrk2.2snrk2.3 double mutant in conferring ABA insensitivity at the stages of seed germination and early seedling growth. Our results suggest that NO negatively regulates ABA signaling in germination and early seedling growth through S-nitrosylation of SnRK2.2 and SnRK2.3. |
| doi_str_mv | 10.1080/15592324.2015.1031939 |
| format | Article |
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In a recent study, we showed that endogenous NO negatively regulates abscisic acid (ABA) signaling in guard cells by inhibiting sucrose nonfermenting 1 (SNF1)-related protein kinase 2.6 (SnRK2.6)/open stomata 1(OST1) through S-nitrosylation. Application of NO breaks seed dormancy and alleviates the inhibitory effect of ABA on seed germination and early seedling growth, but it is unclear how NO functions at the stages of seed germination and early seedling development. Here, we show that like SnRK2.6, SnRK2.2 can be inactivated by S-nitrosoglutathione (GSNO) treatment through S-nitrosylation. SnRK2.2 and the closely related SnRK2.3 are known to play redundant roles in ABA inhibition of seed germination in Arabidopsis. We found that treatment with the NO donor SNP phenocopies the snrk2.2snrk2.3 double mutant in conferring ABA insensitivity at the stages of seed germination and early seedling growth. Our results suggest that NO negatively regulates ABA signaling in germination and early seedling growth through S-nitrosylation of SnRK2.2 and SnRK2.3.</description><identifier>ISSN: 1559-2316</identifier><identifier>ISSN: 1559-2324</identifier><identifier>EISSN: 1559-2324</identifier><identifier>DOI: 10.1080/15592324.2015.1031939</identifier><identifier>PMID: 26024299</identifier><language>eng</language><publisher>United States: Taylor & Francis</publisher><subject>ABA ; Abscisic Acid - pharmacology ; Amino Acid Sequence ; Arabidopsis - metabolism ; Arabidopsis Proteins - metabolism ; Germination - drug effects ; Molecular Sequence Data ; Nitric Oxide - metabolism ; Nitric Oxide - pharmacology ; Nitrosation ; Protein-Serine-Threonine Kinases - metabolism ; S-Nitrosoglutathione - pharmacology ; S-nitrosylation ; seed dormancy ; Seedlings - growth & development ; Seedlings - metabolism ; Seeds - drug effects ; Seeds - growth & development ; Short Communication ; signaling ; SnRK2.2 ; SnRK2.3</subject><ispartof>Plant signaling & behavior, 2015-06, Vol.10 (6), p.e1031939-e1031939</ispartof><rights>2015 Taylor & Francis Group, LLC 2015</rights><rights>2015 Taylor & Francis Group, LLC 2015 Taylor & Francis Group, LLC</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c468t-21d591cfa48782d78c4f5835bb97b02722e8495c2a04fc1e72b01e6ac8dc29b13</citedby><cites>FETCH-LOGICAL-c468t-21d591cfa48782d78c4f5835bb97b02722e8495c2a04fc1e72b01e6ac8dc29b13</cites><orcidid>0000-0001-5134-731X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4622540/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4622540/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26024299$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Pengcheng</creatorcontrib><creatorcontrib>Zhu, Jian-Kang</creatorcontrib><creatorcontrib>Lang, Zhaobo</creatorcontrib><title>Nitric oxide suppresses the inhibitory effect of abscisic acid on seed germination by S-nitrosylation of SnRK2 proteins</title><title>Plant signaling & behavior</title><addtitle>Plant Signal Behav</addtitle><description>Nitric oxide (NO) plays important roles in plant development, and biotic and abiotic stress responses. In a recent study, we showed that endogenous NO negatively regulates abscisic acid (ABA) signaling in guard cells by inhibiting sucrose nonfermenting 1 (SNF1)-related protein kinase 2.6 (SnRK2.6)/open stomata 1(OST1) through S-nitrosylation. Application of NO breaks seed dormancy and alleviates the inhibitory effect of ABA on seed germination and early seedling growth, but it is unclear how NO functions at the stages of seed germination and early seedling development. Here, we show that like SnRK2.6, SnRK2.2 can be inactivated by S-nitrosoglutathione (GSNO) treatment through S-nitrosylation. SnRK2.2 and the closely related SnRK2.3 are known to play redundant roles in ABA inhibition of seed germination in Arabidopsis. We found that treatment with the NO donor SNP phenocopies the snrk2.2snrk2.3 double mutant in conferring ABA insensitivity at the stages of seed germination and early seedling growth. Our results suggest that NO negatively regulates ABA signaling in germination and early seedling growth through S-nitrosylation of SnRK2.2 and SnRK2.3.</description><subject>ABA</subject><subject>Abscisic Acid - pharmacology</subject><subject>Amino Acid Sequence</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Germination - drug effects</subject><subject>Molecular Sequence Data</subject><subject>Nitric Oxide - metabolism</subject><subject>Nitric Oxide - pharmacology</subject><subject>Nitrosation</subject><subject>Protein-Serine-Threonine Kinases - metabolism</subject><subject>S-Nitrosoglutathione - pharmacology</subject><subject>S-nitrosylation</subject><subject>seed dormancy</subject><subject>Seedlings - growth & development</subject><subject>Seedlings - metabolism</subject><subject>Seeds - drug effects</subject><subject>Seeds - growth & development</subject><subject>Short Communication</subject><subject>signaling</subject><subject>SnRK2.2</subject><subject>SnRK2.3</subject><issn>1559-2316</issn><issn>1559-2324</issn><issn>1559-2324</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc2OFCEUhYnROD_6CBqWbmoECqqKjdFM1DFONHF0TYC6TGOqoAR6xnp76XRPRzeuIOeecy7kQ-gFJReUDOQ1FUKylvELRqioUktlKx-h053e7AaPj3fanaCznH8SwtuekKfohHWEcSblKbr_4kvyFsfffgSct8uSIGfIuGwA-7DxxpeYVgzOgS04OqxNtj7XiLZ-xDHgDDDiW0izD7r4KpgV3zSh9sa8Tnup5m7Ct88MLykW8CE_Q0-cnjI8P5zn6MeH998vr5rrrx8_Xb67bizvhtIwOgpJrdN86Ac29oPlTgytMEb2hrCeMRi4FJZpwp2l0DNDKHTaDqNl0tD2HL3Z9y5bM8NoIZSkJ7UkP-u0qqi9-ncS_EbdxjvFO8YEJ7Xg1aEgxV9byEXNPluYJh0gbrOinaSEdqST1Sr2Vlt_nhO44xpK1A6aeoCmdtDUAVrNvfz7jcfUA6VqeLs3-OBimvV9TNOoil6nmFzSofJQ7f93_AHJVaju</recordid><startdate>20150603</startdate><enddate>20150603</enddate><creator>Wang, Pengcheng</creator><creator>Zhu, Jian-Kang</creator><creator>Lang, Zhaobo</creator><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-5134-731X</orcidid></search><sort><creationdate>20150603</creationdate><title>Nitric oxide suppresses the inhibitory effect of abscisic acid on seed germination by S-nitrosylation of SnRK2 proteins</title><author>Wang, Pengcheng ; Zhu, Jian-Kang ; Lang, Zhaobo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c468t-21d591cfa48782d78c4f5835bb97b02722e8495c2a04fc1e72b01e6ac8dc29b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>ABA</topic><topic>Abscisic Acid - pharmacology</topic><topic>Amino Acid Sequence</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Germination - drug effects</topic><topic>Molecular Sequence Data</topic><topic>Nitric Oxide - metabolism</topic><topic>Nitric Oxide - pharmacology</topic><topic>Nitrosation</topic><topic>Protein-Serine-Threonine Kinases - metabolism</topic><topic>S-Nitrosoglutathione - pharmacology</topic><topic>S-nitrosylation</topic><topic>seed dormancy</topic><topic>Seedlings - growth & development</topic><topic>Seedlings - metabolism</topic><topic>Seeds - drug effects</topic><topic>Seeds - growth & development</topic><topic>Short Communication</topic><topic>signaling</topic><topic>SnRK2.2</topic><topic>SnRK2.3</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Pengcheng</creatorcontrib><creatorcontrib>Zhu, Jian-Kang</creatorcontrib><creatorcontrib>Lang, Zhaobo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant signaling & behavior</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Pengcheng</au><au>Zhu, Jian-Kang</au><au>Lang, Zhaobo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nitric oxide suppresses the inhibitory effect of abscisic acid on seed germination by S-nitrosylation of SnRK2 proteins</atitle><jtitle>Plant signaling & behavior</jtitle><addtitle>Plant Signal Behav</addtitle><date>2015-06-03</date><risdate>2015</risdate><volume>10</volume><issue>6</issue><spage>e1031939</spage><epage>e1031939</epage><pages>e1031939-e1031939</pages><issn>1559-2316</issn><issn>1559-2324</issn><eissn>1559-2324</eissn><abstract>Nitric oxide (NO) plays important roles in plant development, and biotic and abiotic stress responses. In a recent study, we showed that endogenous NO negatively regulates abscisic acid (ABA) signaling in guard cells by inhibiting sucrose nonfermenting 1 (SNF1)-related protein kinase 2.6 (SnRK2.6)/open stomata 1(OST1) through S-nitrosylation. Application of NO breaks seed dormancy and alleviates the inhibitory effect of ABA on seed germination and early seedling growth, but it is unclear how NO functions at the stages of seed germination and early seedling development. Here, we show that like SnRK2.6, SnRK2.2 can be inactivated by S-nitrosoglutathione (GSNO) treatment through S-nitrosylation. SnRK2.2 and the closely related SnRK2.3 are known to play redundant roles in ABA inhibition of seed germination in Arabidopsis. We found that treatment with the NO donor SNP phenocopies the snrk2.2snrk2.3 double mutant in conferring ABA insensitivity at the stages of seed germination and early seedling growth. Our results suggest that NO negatively regulates ABA signaling in germination and early seedling growth through S-nitrosylation of SnRK2.2 and SnRK2.3.</abstract><cop>United States</cop><pub>Taylor & Francis</pub><pmid>26024299</pmid><doi>10.1080/15592324.2015.1031939</doi><orcidid>https://orcid.org/0000-0001-5134-731X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | ABA Abscisic Acid - pharmacology Amino Acid Sequence Arabidopsis - metabolism Arabidopsis Proteins - metabolism Germination - drug effects Molecular Sequence Data Nitric Oxide - metabolism Nitric Oxide - pharmacology Nitrosation Protein-Serine-Threonine Kinases - metabolism S-Nitrosoglutathione - pharmacology S-nitrosylation seed dormancy Seedlings - growth & development Seedlings - metabolism Seeds - drug effects Seeds - growth & development Short Communication signaling SnRK2.2 SnRK2.3 |
| title | Nitric oxide suppresses the inhibitory effect of abscisic acid on seed germination by S-nitrosylation of SnRK2 proteins |
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