Improvement and optimization of a T-cell-dependent antibody response (TDAR) method for BALB/c mice using keyhole limpet hemocyanin (KLH) as specific antigen

Although T-cell-dependent antibody response (TDAR) assays with keyhole limpet hemocyanin (KLH) as specific antigen have many advantages, most experiments produce qualitative results based on antibody titers. It was hypothesized that if experimental conditions (like antigen coating concentration, ser...

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Veröffentlicht in:	Journal of immunotoxicology 2019-01, Vol.16 (1), p.149-154
Hauptverfasser:	Chang, Penghuan, Huang, Ling, Huang, Mianqing, Tian, Shuhong, Yang, Zhaoxin
Format:	Artikel
Sprache:	eng
Schlagworte:	Adjuvants, Immunologic - administration & dosage Adjuvants, Immunologic - toxicity Animals Antibody Formation - drug effects Biological Assay - methods Dose-Response Relationship, Drug Enzyme-Linked Immunosorbent Assay - methods Female Hemocyanins - administration & dosage Hemocyanins - immunology Hemocyanins - toxicity Immunoglobulin G - blood Immunoglobulin G - immunology Immunoglobulin G - isolation & purification Keyhole limpet hemocyanin Limit of Detection Male Mice Mice, Inbred BALB C optical density value qualitative analysis Sensitivity and Specificity T-cell-dependent antibody response T-Lymphocytes - drug effects T-Lymphocytes - immunology Toxicity Tests - methods
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