Emerging roles of circRNA_NEK6 targeting miR-370-3p in the proliferation and invasion of thyroid cancer via Wnt signaling pathway
Objective: To identify the significantly altered circRNAs and mRNAs in thyroid cancer, investigate their target miRNAs and determine their biological functions. Methods: The differentially expressed circRNAs, mRNAs and pathways in thyroid cancer were identified by microarray analysis and gene set en...
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| Veröffentlicht in: | Cancer biology & therapy 2018-12, Vol.19 (12), p.1139-1152 |
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| creator | Chen, Fukun Feng, Zhiping Zhu, Jialun Liu, Pengjie Yang, Chuanzhou Huang, Rongkai Deng, Zhiyong |
| description | Objective: To identify the significantly altered circRNAs and mRNAs in thyroid cancer, investigate their target miRNAs and determine their biological functions.
Methods: The differentially expressed circRNAs, mRNAs and pathways in thyroid cancer were identified by microarray analysis and gene set enrichment analysis (GSEA). The correlative circRNAs and mRNAs were found out through Pearson correlative analysis. The common target miRNAs of circNEK6 and FZD8 related to thyroid cancer was screened out through Targetscan, miRanda and HMDD analysis. The mRNA and protein expressions in thyroid cancer tissues and cells were detected by qRT-PCR and western blot. CircRNA was confirmed by the RNase R digestion and nucleic acid electrophoresis. The target relationships were verified by the dual luciferase reporter assay. Cell viability, invasion and apoptosis were determined by MTT assay, Transwell assay and flow cytometry, respectively.
Results: CircNEK6 and FZD8 were significantly up-regulated in thyroid cancer, with strong correlations. The Wnt signaling pathway was activated in thyroid cancer. MiR-370-3p was the common target miRNA of circNEK6 and FZD8, and it was down-regulated in thyroid cancer. Overexpression of circNEK6 and FZD8 could promote the growth and invasion of thyroid cancer cells, while up-regulation of miR-370-3p could suppress thyroid cancer progression and inhibit the Wnt signaling pathway. MiR-370-3p's effect on thyroid cancer cells could be rescued by circNEK6 or FZD8.
Conclusion: CircNEK6 promoted the progression of thyroid cancer through up-regulating FZD8 and activating Wnt signaling pathway by targeting miR-370-3p. |
| doi_str_mv | 10.1080/15384047.2018.1480888 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1080_15384047_2018_1480888</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2103669782</sourcerecordid><originalsourceid>FETCH-LOGICAL-c534t-e0b3223fe970afc4d26d047734c776e019420e434e628e113ae17ec5206d34473</originalsourceid><addsrcrecordid>eNp9UUtv1DAQjhCIlsJPAPnIJVu_YjsXRFVtoaIqUgXiaLnOJGuU2MH2brVH_jmOdlvBhdN49D1mPF9VvSV4RbDC56RhimMuVxQTtSJcYaXUs-qUNE1Tq0aK58ubqXohnVSvUvqJMZVUtC-rE4Yplkq0p9Xv9QRxcH5AMYyQUOiRddHe3V7o2_UXgbKJA-QFn9xdzSSu2YycR3kDaC4S10M02QWPjO8KsDNpaYpN3uxjcB2yxluIaOcM-uEzSm7wZlwMZ5M3D2b_unrRmzHBm2M9q75frb9dfq5vvn66vry4qW3DeK4B3zNKWQ-txKa3vKOiKz-TjFspBWDScoqBMw6CKiCEGSASbEOx6Bjnkp1VHw6-8_Z-gs6Cz9GMeo5uMnGvg3H6X8S7jR7CTgtWLkwWg_dHgxh-bSFlPblkYRyNh7BNmhLMhGilooXaHKg2hpQi9E9jCNZLfPoxPr3Ep4_xFd27v3d8Uj3mVQgfDwTn-xAn8xDi2Ols9mOIfSyXdkmz_8_4A-AVqnc</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2103669782</pqid></control><display><type>article</type><title>Emerging roles of circRNA_NEK6 targeting miR-370-3p in the proliferation and invasion of thyroid cancer via Wnt signaling pathway</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Chen, Fukun ; Feng, Zhiping ; Zhu, Jialun ; Liu, Pengjie ; Yang, Chuanzhou ; Huang, Rongkai ; Deng, Zhiyong</creator><creatorcontrib>Chen, Fukun ; Feng, Zhiping ; Zhu, Jialun ; Liu, Pengjie ; Yang, Chuanzhou ; Huang, Rongkai ; Deng, Zhiyong</creatorcontrib><description>Objective: To identify the significantly altered circRNAs and mRNAs in thyroid cancer, investigate their target miRNAs and determine their biological functions.
Methods: The differentially expressed circRNAs, mRNAs and pathways in thyroid cancer were identified by microarray analysis and gene set enrichment analysis (GSEA). The correlative circRNAs and mRNAs were found out through Pearson correlative analysis. The common target miRNAs of circNEK6 and FZD8 related to thyroid cancer was screened out through Targetscan, miRanda and HMDD analysis. The mRNA and protein expressions in thyroid cancer tissues and cells were detected by qRT-PCR and western blot. CircRNA was confirmed by the RNase R digestion and nucleic acid electrophoresis. The target relationships were verified by the dual luciferase reporter assay. Cell viability, invasion and apoptosis were determined by MTT assay, Transwell assay and flow cytometry, respectively.
Results: CircNEK6 and FZD8 were significantly up-regulated in thyroid cancer, with strong correlations. The Wnt signaling pathway was activated in thyroid cancer. MiR-370-3p was the common target miRNA of circNEK6 and FZD8, and it was down-regulated in thyroid cancer. Overexpression of circNEK6 and FZD8 could promote the growth and invasion of thyroid cancer cells, while up-regulation of miR-370-3p could suppress thyroid cancer progression and inhibit the Wnt signaling pathway. MiR-370-3p's effect on thyroid cancer cells could be rescued by circNEK6 or FZD8.
Conclusion: CircNEK6 promoted the progression of thyroid cancer through up-regulating FZD8 and activating Wnt signaling pathway by targeting miR-370-3p.</description><identifier>ISSN: 1538-4047</identifier><identifier>EISSN: 1555-8576</identifier><identifier>DOI: 10.1080/15384047.2018.1480888</identifier><identifier>PMID: 30207869</identifier><language>eng</language><publisher>United States: Taylor & Francis</publisher><subject>Binding Sites ; Cell Line, Tumor ; Cell Movement - genetics ; Cell Proliferation - genetics ; Cell Survival - genetics ; circRNA ; FZD8 ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Gene Regulatory Networks ; GSEA ; Humans ; KEGG ; MicroRNAs - genetics ; miR-370-3p ; Models, Biological ; NIMA-Related Kinases - genetics ; Research Paper ; RNA - genetics ; RNA Interference ; RNA, Circular ; thyroid cancer ; Thyroid Neoplasms - genetics ; Thyroid Neoplasms - metabolism ; Thyroid Neoplasms - pathology ; Wnt Signaling Pathway</subject><ispartof>Cancer biology & therapy, 2018-12, Vol.19 (12), p.1139-1152</ispartof><rights>2018 Taylor & Francis Group, LLC 2018</rights><rights>2018 Taylor & Francis Group, LLC 2018 Taylor & Francis</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c534t-e0b3223fe970afc4d26d047734c776e019420e434e628e113ae17ec5206d34473</citedby><cites>FETCH-LOGICAL-c534t-e0b3223fe970afc4d26d047734c776e019420e434e628e113ae17ec5206d34473</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6301817/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6301817/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30207869$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Fukun</creatorcontrib><creatorcontrib>Feng, Zhiping</creatorcontrib><creatorcontrib>Zhu, Jialun</creatorcontrib><creatorcontrib>Liu, Pengjie</creatorcontrib><creatorcontrib>Yang, Chuanzhou</creatorcontrib><creatorcontrib>Huang, Rongkai</creatorcontrib><creatorcontrib>Deng, Zhiyong</creatorcontrib><title>Emerging roles of circRNA_NEK6 targeting miR-370-3p in the proliferation and invasion of thyroid cancer via Wnt signaling pathway</title><title>Cancer biology & therapy</title><addtitle>Cancer Biol Ther</addtitle><description>Objective: To identify the significantly altered circRNAs and mRNAs in thyroid cancer, investigate their target miRNAs and determine their biological functions.
Methods: The differentially expressed circRNAs, mRNAs and pathways in thyroid cancer were identified by microarray analysis and gene set enrichment analysis (GSEA). The correlative circRNAs and mRNAs were found out through Pearson correlative analysis. The common target miRNAs of circNEK6 and FZD8 related to thyroid cancer was screened out through Targetscan, miRanda and HMDD analysis. The mRNA and protein expressions in thyroid cancer tissues and cells were detected by qRT-PCR and western blot. CircRNA was confirmed by the RNase R digestion and nucleic acid electrophoresis. The target relationships were verified by the dual luciferase reporter assay. Cell viability, invasion and apoptosis were determined by MTT assay, Transwell assay and flow cytometry, respectively.
Results: CircNEK6 and FZD8 were significantly up-regulated in thyroid cancer, with strong correlations. The Wnt signaling pathway was activated in thyroid cancer. MiR-370-3p was the common target miRNA of circNEK6 and FZD8, and it was down-regulated in thyroid cancer. Overexpression of circNEK6 and FZD8 could promote the growth and invasion of thyroid cancer cells, while up-regulation of miR-370-3p could suppress thyroid cancer progression and inhibit the Wnt signaling pathway. MiR-370-3p's effect on thyroid cancer cells could be rescued by circNEK6 or FZD8.
Conclusion: CircNEK6 promoted the progression of thyroid cancer through up-regulating FZD8 and activating Wnt signaling pathway by targeting miR-370-3p.</description><subject>Binding Sites</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement - genetics</subject><subject>Cell Proliferation - genetics</subject><subject>Cell Survival - genetics</subject><subject>circRNA</subject><subject>FZD8</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Gene Regulatory Networks</subject><subject>GSEA</subject><subject>Humans</subject><subject>KEGG</subject><subject>MicroRNAs - genetics</subject><subject>miR-370-3p</subject><subject>Models, Biological</subject><subject>NIMA-Related Kinases - genetics</subject><subject>Research Paper</subject><subject>RNA - genetics</subject><subject>RNA Interference</subject><subject>RNA, Circular</subject><subject>thyroid cancer</subject><subject>Thyroid Neoplasms - genetics</subject><subject>Thyroid Neoplasms - metabolism</subject><subject>Thyroid Neoplasms - pathology</subject><subject>Wnt Signaling Pathway</subject><issn>1538-4047</issn><issn>1555-8576</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UUtv1DAQjhCIlsJPAPnIJVu_YjsXRFVtoaIqUgXiaLnOJGuU2MH2brVH_jmOdlvBhdN49D1mPF9VvSV4RbDC56RhimMuVxQTtSJcYaXUs-qUNE1Tq0aK58ubqXohnVSvUvqJMZVUtC-rE4Yplkq0p9Xv9QRxcH5AMYyQUOiRddHe3V7o2_UXgbKJA-QFn9xdzSSu2YycR3kDaC4S10M02QWPjO8KsDNpaYpN3uxjcB2yxluIaOcM-uEzSm7wZlwMZ5M3D2b_unrRmzHBm2M9q75frb9dfq5vvn66vry4qW3DeK4B3zNKWQ-txKa3vKOiKz-TjFspBWDScoqBMw6CKiCEGSASbEOx6Bjnkp1VHw6-8_Z-gs6Cz9GMeo5uMnGvg3H6X8S7jR7CTgtWLkwWg_dHgxh-bSFlPblkYRyNh7BNmhLMhGilooXaHKg2hpQi9E9jCNZLfPoxPr3Ep4_xFd27v3d8Uj3mVQgfDwTn-xAn8xDi2Ols9mOIfSyXdkmz_8_4A-AVqnc</recordid><startdate>20181202</startdate><enddate>20181202</enddate><creator>Chen, Fukun</creator><creator>Feng, Zhiping</creator><creator>Zhu, Jialun</creator><creator>Liu, Pengjie</creator><creator>Yang, Chuanzhou</creator><creator>Huang, Rongkai</creator><creator>Deng, Zhiyong</creator><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20181202</creationdate><title>Emerging roles of circRNA_NEK6 targeting miR-370-3p in the proliferation and invasion of thyroid cancer via Wnt signaling pathway</title><author>Chen, Fukun ; Feng, Zhiping ; Zhu, Jialun ; Liu, Pengjie ; Yang, Chuanzhou ; Huang, Rongkai ; Deng, Zhiyong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c534t-e0b3223fe970afc4d26d047734c776e019420e434e628e113ae17ec5206d34473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Binding Sites</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement - genetics</topic><topic>Cell Proliferation - genetics</topic><topic>Cell Survival - genetics</topic><topic>circRNA</topic><topic>FZD8</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Gene Regulatory Networks</topic><topic>GSEA</topic><topic>Humans</topic><topic>KEGG</topic><topic>MicroRNAs - genetics</topic><topic>miR-370-3p</topic><topic>Models, Biological</topic><topic>NIMA-Related Kinases - genetics</topic><topic>Research Paper</topic><topic>RNA - genetics</topic><topic>RNA Interference</topic><topic>RNA, Circular</topic><topic>thyroid cancer</topic><topic>Thyroid Neoplasms - genetics</topic><topic>Thyroid Neoplasms - metabolism</topic><topic>Thyroid Neoplasms - pathology</topic><topic>Wnt Signaling Pathway</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Fukun</creatorcontrib><creatorcontrib>Feng, Zhiping</creatorcontrib><creatorcontrib>Zhu, Jialun</creatorcontrib><creatorcontrib>Liu, Pengjie</creatorcontrib><creatorcontrib>Yang, Chuanzhou</creatorcontrib><creatorcontrib>Huang, Rongkai</creatorcontrib><creatorcontrib>Deng, Zhiyong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cancer biology & therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Fukun</au><au>Feng, Zhiping</au><au>Zhu, Jialun</au><au>Liu, Pengjie</au><au>Yang, Chuanzhou</au><au>Huang, Rongkai</au><au>Deng, Zhiyong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Emerging roles of circRNA_NEK6 targeting miR-370-3p in the proliferation and invasion of thyroid cancer via Wnt signaling pathway</atitle><jtitle>Cancer biology & therapy</jtitle><addtitle>Cancer Biol Ther</addtitle><date>2018-12-02</date><risdate>2018</risdate><volume>19</volume><issue>12</issue><spage>1139</spage><epage>1152</epage><pages>1139-1152</pages><issn>1538-4047</issn><eissn>1555-8576</eissn><abstract>Objective: To identify the significantly altered circRNAs and mRNAs in thyroid cancer, investigate their target miRNAs and determine their biological functions.
Methods: The differentially expressed circRNAs, mRNAs and pathways in thyroid cancer were identified by microarray analysis and gene set enrichment analysis (GSEA). The correlative circRNAs and mRNAs were found out through Pearson correlative analysis. The common target miRNAs of circNEK6 and FZD8 related to thyroid cancer was screened out through Targetscan, miRanda and HMDD analysis. The mRNA and protein expressions in thyroid cancer tissues and cells were detected by qRT-PCR and western blot. CircRNA was confirmed by the RNase R digestion and nucleic acid electrophoresis. The target relationships were verified by the dual luciferase reporter assay. Cell viability, invasion and apoptosis were determined by MTT assay, Transwell assay and flow cytometry, respectively.
Results: CircNEK6 and FZD8 were significantly up-regulated in thyroid cancer, with strong correlations. The Wnt signaling pathway was activated in thyroid cancer. MiR-370-3p was the common target miRNA of circNEK6 and FZD8, and it was down-regulated in thyroid cancer. Overexpression of circNEK6 and FZD8 could promote the growth and invasion of thyroid cancer cells, while up-regulation of miR-370-3p could suppress thyroid cancer progression and inhibit the Wnt signaling pathway. MiR-370-3p's effect on thyroid cancer cells could be rescued by circNEK6 or FZD8.
Conclusion: CircNEK6 promoted the progression of thyroid cancer through up-regulating FZD8 and activating Wnt signaling pathway by targeting miR-370-3p.</abstract><cop>United States</cop><pub>Taylor & Francis</pub><pmid>30207869</pmid><doi>10.1080/15384047.2018.1480888</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Binding Sites Cell Line, Tumor Cell Movement - genetics Cell Proliferation - genetics Cell Survival - genetics circRNA FZD8 Gene Expression Profiling Gene Expression Regulation, Neoplastic Gene Regulatory Networks GSEA Humans KEGG MicroRNAs - genetics miR-370-3p Models, Biological NIMA-Related Kinases - genetics Research Paper RNA - genetics RNA Interference RNA, Circular thyroid cancer Thyroid Neoplasms - genetics Thyroid Neoplasms - metabolism Thyroid Neoplasms - pathology Wnt Signaling Pathway |
| title | Emerging roles of circRNA_NEK6 targeting miR-370-3p in the proliferation and invasion of thyroid cancer via Wnt signaling pathway |
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