Synthesis, Biophysical and Biological Evaluations of Novel Antisense Oligonucleosides Containing Dephosphono-Internucleosidic Linkages
Efficient large-scale syntheses of methylene(methylimino) (MMI) linked mixed base nucleosidic dimers have been accomplished. These dimers were successfully incorporated into deoxyoligonucleotides by automated solid-support synthesis. The hybridization properties, nuclease stability, RNase H mediated...
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| Veröffentlicht in: | Nucleosides & nucleotides 1995-05, Vol.14 (3-5), p.1087-1090 |
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| container_issue | 3-5 |
| container_start_page | 1087 |
| container_title | Nucleosides & nucleotides |
| container_volume | 14 |
| creator | Sanghvi, Yogesh S. Bellon, Laurent Morvan, Francęois Hoshiko, Tomonori Swayze, Eric Cummins, Lendell Freier, Susan Dean, Nicholas Monia, Brett Griffey, Richard H. Cook, P. Dan |
| description | Efficient large-scale syntheses of methylene(methylimino) (MMI) linked mixed base nucleosidic dimers have been accomplished. These dimers were successfully incorporated into deoxyoligonucleotides by automated solid-support synthesis. The hybridization properties, nuclease stability, RNase H mediated cleavage and in vitro biological activity of novel chimeric oligomers have been studied. The biophysical and biological evaluation of these chimeric oligomers containing MMI linkages suggests that MMI is a promising chemical modification of the backbone linkage for the construction of antisense molecules useful as therapeutics. |
| doi_str_mv | 10.1080/15257779508012540 |
| format | Article |
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| language | eng |
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| source | Taylor & Francis:Master (3349 titles) |
| title | Synthesis, Biophysical and Biological Evaluations of Novel Antisense Oligonucleosides Containing Dephosphono-Internucleosidic Linkages |
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