A comparative histopathological and immunohistochemical study of Survivin and Ki-67 proteins in glial tumours
Survivin is a bifunctional protein which regulates cell division and inhibits apoptosis. Survivin is a member of the inhibitor of apoptosis protein (IAP) family. Expression of survivin has been shown to be responsible for apoptosis and resistance to ionizing radiation. The aim of the present study w...
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| Veröffentlicht in: | Biotechnology, biotechnological equipment biotechnological equipment, 2019-01, Vol.33 (1), p.504-509 |
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| creator | Topyalin, Nur Budak, Metin Ozbay, Nurver Yildiz, Mustafa Kaner, Tuncay Aydin, Abdullah Gezen, Ahmet Ferruh |
| description | Survivin is a bifunctional protein which regulates cell division and inhibits apoptosis. Survivin is a member of the inhibitor of apoptosis protein (IAP) family. Expression of survivin has been shown to be responsible for apoptosis and resistance to ionizing radiation. The aim of the present study was to investigate the association between −31 G/C promoter polymorphism, survivin protein and glial tumour grading, and to compare survivin versus Ki-67 as a marker. In this study, DNA was isolated from paraffin-embedded sections of 29 patients diagnosed with glial tumours. Survivin gene promoter −31 G/C polymorphism was investigated using PCR-RFLP. For the analysis, 10 µm sections were stained with survivin protein and Ki-67 antibody. Immunohistochemical staining was performed. Survivin showed a positive correlation with Ki-67 (r = 0.604; p = 0.001). The tumour grades correlated with survivin; however, the relationship was not statistically significant (r = 0.345; p > 0.05). We found a significant correlation between tumour grades and Ki-67 (r = 0.663; p |
| doi_str_mv | 10.1080/13102818.2019.1591931 |
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Survivin is a member of the inhibitor of apoptosis protein (IAP) family. Expression of survivin has been shown to be responsible for apoptosis and resistance to ionizing radiation. The aim of the present study was to investigate the association between −31 G/C promoter polymorphism, survivin protein and glial tumour grading, and to compare survivin versus Ki-67 as a marker. In this study, DNA was isolated from paraffin-embedded sections of 29 patients diagnosed with glial tumours. Survivin gene promoter −31 G/C polymorphism was investigated using PCR-RFLP. For the analysis, 10 µm sections were stained with survivin protein and Ki-67 antibody. Immunohistochemical staining was performed. Survivin showed a positive correlation with Ki-67 (r = 0.604; p = 0.001). The tumour grades correlated with survivin; however, the relationship was not statistically significant (r = 0.345; p > 0.05). We found a significant correlation between tumour grades and Ki-67 (r = 0.663; p < 0.01), suggesting that Ki-67 is a more sensitive marker compared to survivin.</description><identifier>ISSN: 1310-2818</identifier><identifier>EISSN: 1314-3530</identifier><identifier>DOI: 10.1080/13102818.2019.1591931</identifier><language>eng</language><publisher>Sofia: Taylor & Francis</publisher><subject>Antibodies ; Apoptosis ; Cell division ; Correlation ; Deoxyribonucleic acid ; DNA ; Gene polymorphism ; glial tumour ; IAP protein ; Immunohistochemistry ; Ionizing radiation ; Ki-67 ; Markers ; Paraffin ; Paraffins ; Polymerase chain reaction ; Polymorphism ; Proteins ; Radiation tolerance ; Restriction fragment length polymorphism ; Statistical analysis ; Survival ; Survivin ; Tumors</subject><ispartof>Biotechnology, biotechnological equipment, 2019-01, Vol.33 (1), p.504-509</ispartof><rights>2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. 2019</rights><rights>2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This work is licensed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c399t-4a297830ec34572fcb221ec3c469f42aecb6d14874bb0823314f9ec2238d42193</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/13102818.2019.1591931$$EPDF$$P50$$Ginformaworld$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/13102818.2019.1591931$$EHTML$$P50$$Ginformaworld$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,860,2095,27481,27903,27904,59119,59120</link.rule.ids></links><search><creatorcontrib>Topyalin, Nur</creatorcontrib><creatorcontrib>Budak, Metin</creatorcontrib><creatorcontrib>Ozbay, Nurver</creatorcontrib><creatorcontrib>Yildiz, Mustafa</creatorcontrib><creatorcontrib>Kaner, Tuncay</creatorcontrib><creatorcontrib>Aydin, Abdullah</creatorcontrib><creatorcontrib>Gezen, Ahmet Ferruh</creatorcontrib><title>A comparative histopathological and immunohistochemical study of Survivin and Ki-67 proteins in glial tumours</title><title>Biotechnology, biotechnological equipment</title><description>Survivin is a bifunctional protein which regulates cell division and inhibits apoptosis. Survivin is a member of the inhibitor of apoptosis protein (IAP) family. Expression of survivin has been shown to be responsible for apoptosis and resistance to ionizing radiation. The aim of the present study was to investigate the association between −31 G/C promoter polymorphism, survivin protein and glial tumour grading, and to compare survivin versus Ki-67 as a marker. In this study, DNA was isolated from paraffin-embedded sections of 29 patients diagnosed with glial tumours. Survivin gene promoter −31 G/C polymorphism was investigated using PCR-RFLP. For the analysis, 10 µm sections were stained with survivin protein and Ki-67 antibody. Immunohistochemical staining was performed. Survivin showed a positive correlation with Ki-67 (r = 0.604; p = 0.001). The tumour grades correlated with survivin; however, the relationship was not statistically significant (r = 0.345; p > 0.05). We found a significant correlation between tumour grades and Ki-67 (r = 0.663; p < 0.01), suggesting that Ki-67 is a more sensitive marker compared to survivin.</description><subject>Antibodies</subject><subject>Apoptosis</subject><subject>Cell division</subject><subject>Correlation</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Gene polymorphism</subject><subject>glial tumour</subject><subject>IAP protein</subject><subject>Immunohistochemistry</subject><subject>Ionizing radiation</subject><subject>Ki-67</subject><subject>Markers</subject><subject>Paraffin</subject><subject>Paraffins</subject><subject>Polymerase chain reaction</subject><subject>Polymorphism</subject><subject>Proteins</subject><subject>Radiation tolerance</subject><subject>Restriction fragment length polymorphism</subject><subject>Statistical analysis</subject><subject>Survival</subject><subject>Survivin</subject><subject>Tumors</subject><issn>1310-2818</issn><issn>1314-3530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><sourceid>DOA</sourceid><recordid>eNp9Uctu2zAQFIIUiOPmEwII6FnuLh8WeUsQ9GE0QA9NzgRFUTYNSXRJyoX_PrSc9tgTl7OzszuYorhHWCEI-IwUgQgUKwIoV8glSopXxSLjrKKcwvVcQ3Um3RS3Me4BagCsF8XwWBo_HHTQyR1tuXMx-YNOO9_7rTO6L_XYlm4YptHPPbOzw4zHNLWn0nflrykc3dGNM_OHq9Z1eQg-WTfGMqPb3mV2mgY_hfix-NDpPtq793dZvH798vL0vXr--W3z9PhcGSplqpgmshYUrKGM16QzDSGYP4atZceItqZZt8hEzZoGBKHZZyetIYSKlpHsfllsLrqt13t1CG7Q4aS8dmoGfNgqHZIzvVVNLTiXwLUAZEhAYEsaMEagZOt8Rtb6dNHKrn5PNia1z1bGfL4ilCNwSYXILH5hmeBjDLb7txVBnVNSf1NS55TUe0p57uEy58bOh0H_8aFvVdKn3ocu6NG4qOj_Jd4AbVOX-Q</recordid><startdate>20190101</startdate><enddate>20190101</enddate><creator>Topyalin, Nur</creator><creator>Budak, Metin</creator><creator>Ozbay, Nurver</creator><creator>Yildiz, Mustafa</creator><creator>Kaner, Tuncay</creator><creator>Aydin, Abdullah</creator><creator>Gezen, Ahmet Ferruh</creator><general>Taylor & Francis</general><general>Taylor & Francis Ltd</general><general>Taylor & Francis Group</general><scope>0YH</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7ST</scope><scope>7XB</scope><scope>8FD</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>M2O</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>SOI</scope><scope>DOA</scope></search><sort><creationdate>20190101</creationdate><title>A comparative histopathological and immunohistochemical study of Survivin and Ki-67 proteins in glial tumours</title><author>Topyalin, Nur ; Budak, Metin ; Ozbay, Nurver ; Yildiz, Mustafa ; Kaner, Tuncay ; Aydin, Abdullah ; Gezen, Ahmet Ferruh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c399t-4a297830ec34572fcb221ec3c469f42aecb6d14874bb0823314f9ec2238d42193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Antibodies</topic><topic>Apoptosis</topic><topic>Cell division</topic><topic>Correlation</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Gene polymorphism</topic><topic>glial tumour</topic><topic>IAP protein</topic><topic>Immunohistochemistry</topic><topic>Ionizing radiation</topic><topic>Ki-67</topic><topic>Markers</topic><topic>Paraffin</topic><topic>Paraffins</topic><topic>Polymerase chain reaction</topic><topic>Polymorphism</topic><topic>Proteins</topic><topic>Radiation tolerance</topic><topic>Restriction fragment length polymorphism</topic><topic>Statistical analysis</topic><topic>Survival</topic><topic>Survivin</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Topyalin, Nur</creatorcontrib><creatorcontrib>Budak, Metin</creatorcontrib><creatorcontrib>Ozbay, Nurver</creatorcontrib><creatorcontrib>Yildiz, Mustafa</creatorcontrib><creatorcontrib>Kaner, Tuncay</creatorcontrib><creatorcontrib>Aydin, Abdullah</creatorcontrib><creatorcontrib>Gezen, Ahmet Ferruh</creatorcontrib><collection>Taylor & Francis Open Access</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Technology Research Database</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Research Library</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Environment Abstracts</collection><collection>DOAJ</collection><jtitle>Biotechnology, biotechnological equipment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Topyalin, Nur</au><au>Budak, Metin</au><au>Ozbay, Nurver</au><au>Yildiz, Mustafa</au><au>Kaner, Tuncay</au><au>Aydin, Abdullah</au><au>Gezen, Ahmet Ferruh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A comparative histopathological and immunohistochemical study of Survivin and Ki-67 proteins in glial tumours</atitle><jtitle>Biotechnology, biotechnological equipment</jtitle><date>2019-01-01</date><risdate>2019</risdate><volume>33</volume><issue>1</issue><spage>504</spage><epage>509</epage><pages>504-509</pages><issn>1310-2818</issn><eissn>1314-3530</eissn><abstract>Survivin is a bifunctional protein which regulates cell division and inhibits apoptosis. Survivin is a member of the inhibitor of apoptosis protein (IAP) family. Expression of survivin has been shown to be responsible for apoptosis and resistance to ionizing radiation. The aim of the present study was to investigate the association between −31 G/C promoter polymorphism, survivin protein and glial tumour grading, and to compare survivin versus Ki-67 as a marker. In this study, DNA was isolated from paraffin-embedded sections of 29 patients diagnosed with glial tumours. Survivin gene promoter −31 G/C polymorphism was investigated using PCR-RFLP. For the analysis, 10 µm sections were stained with survivin protein and Ki-67 antibody. Immunohistochemical staining was performed. Survivin showed a positive correlation with Ki-67 (r = 0.604; p = 0.001). The tumour grades correlated with survivin; however, the relationship was not statistically significant (r = 0.345; p > 0.05). We found a significant correlation between tumour grades and Ki-67 (r = 0.663; p < 0.01), suggesting that Ki-67 is a more sensitive marker compared to survivin.</abstract><cop>Sofia</cop><pub>Taylor & Francis</pub><doi>10.1080/13102818.2019.1591931</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Antibodies Apoptosis Cell division Correlation Deoxyribonucleic acid DNA Gene polymorphism glial tumour IAP protein Immunohistochemistry Ionizing radiation Ki-67 Markers Paraffin Paraffins Polymerase chain reaction Polymorphism Proteins Radiation tolerance Restriction fragment length polymorphism Statistical analysis Survival Survivin Tumors |
| title | A comparative histopathological and immunohistochemical study of Survivin and Ki-67 proteins in glial tumours |
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