Cloning and expression analysis of eIF-5A gene in Apocynum venetum
To understand the effects of the eIF-5A gene in response to abiotic stress, the 909 bp full-length cDNA of eIF-5A, including a 480 bp open reading frame encoding 159 amino-acid (aa) residues, was isolated from the leaf of herbal plant (Apocynum venetum) by rapid-amplification of cDNA ends. The deduc...
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| Veröffentlicht in: | Biotechnology, biotechnological equipment biotechnological equipment, 2016-07, Vol.30 (4), p.677-684 |
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| description | To understand the effects of the eIF-5A gene in response to abiotic stress, the 909 bp full-length cDNA of eIF-5A, including a 480 bp open reading frame encoding 159 amino-acid (aa) residues, was isolated from the leaf of herbal plant (Apocynum venetum) by rapid-amplification of cDNA ends. The deduced molecular weight of the encoding protein was 17.48 kDa with a theoretical pI of 5.61 and predicted no signal peptide. Real-time polymerase chain reaction analysis revealed that AveIF-5A gene expression was induced by cold, salt and drought stress. To determine the biological function of this gene, recombinant plasmids expressing AveIF-5A and AveIF-5A1 (86-156 aa truncated polypeptide) were used to transform Escherichia coli. The analysis of the growth curves of recombinant E. coli revealed that AveIF-5A and AveIF-5A1 improved the resistance of transformed E. coli to low-temperature, drought and salt stress. These results could provide experimental evidence of the function of the AveIF-5A gene as a valuable gene resource in plant resistance-breeding. |
| doi_str_mv | 10.1080/13102818.2016.1172944 |
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The deduced molecular weight of the encoding protein was 17.48 kDa with a theoretical pI of 5.61 and predicted no signal peptide. Real-time polymerase chain reaction analysis revealed that AveIF-5A gene expression was induced by cold, salt and drought stress. To determine the biological function of this gene, recombinant plasmids expressing AveIF-5A and AveIF-5A1 (86-156 aa truncated polypeptide) were used to transform Escherichia coli. The analysis of the growth curves of recombinant E. coli revealed that AveIF-5A and AveIF-5A1 improved the resistance of transformed E. coli to low-temperature, drought and salt stress. These results could provide experimental evidence of the function of the AveIF-5A gene as a valuable gene resource in plant resistance-breeding.</description><identifier>ISSN: 1310-2818</identifier><identifier>EISSN: 1314-3530</identifier><identifier>DOI: 10.1080/13102818.2016.1172944</identifier><language>eng</language><publisher>Sofia: Taylor & Francis</publisher><subject>Amino acids ; Apocynum ; Apocynum venetum ; Cloning ; Drought ; E coli ; eIF-5A ; Gene expression ; Growth curves ; Low temperature ; Low temperature resistance ; Molecular weight ; Plant breeding ; Plant resistance ; Plasmids ; Polymerase chain reaction ; Polypeptides ; prokaryotic expression ; RACE ; real-time PCR ; Stress</subject><ispartof>Biotechnology, biotechnological equipment, 2016-07, Vol.30 (4), p.677-684</ispartof><rights>2016 The Author(s). Published by Taylor & Francis. 2016</rights><rights>2016 The Author(s). Published by Taylor & Francis. This work is licensed under the Creative Commons Attribution – Non-Commercial – No Derivatives License http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-46e22b5307e9db884a593fc9bce698d4c2e4e68753c83e2cd795b75471f68c9b3</citedby><cites>FETCH-LOGICAL-c404t-46e22b5307e9db884a593fc9bce698d4c2e4e68753c83e2cd795b75471f68c9b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/13102818.2016.1172944$$EPDF$$P50$$Ginformaworld$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/13102818.2016.1172944$$EHTML$$P50$$Ginformaworld$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,864,2102,27502,27924,27925,59143,59144</link.rule.ids></links><search><creatorcontrib>Wang, Lei</creatorcontrib><creatorcontrib>Huang, Guo-Qing</creatorcontrib><creatorcontrib>Sun, Yao</creatorcontrib><creatorcontrib>Li, Yao</creatorcontrib><creatorcontrib>Yao, Wen-Jing</creatorcontrib><creatorcontrib>Jiang, Ting-Bo</creatorcontrib><title>Cloning and expression analysis of eIF-5A gene in Apocynum venetum</title><title>Biotechnology, biotechnological equipment</title><description>To understand the effects of the eIF-5A gene in response to abiotic stress, the 909 bp full-length cDNA of eIF-5A, including a 480 bp open reading frame encoding 159 amino-acid (aa) residues, was isolated from the leaf of herbal plant (Apocynum venetum) by rapid-amplification of cDNA ends. The deduced molecular weight of the encoding protein was 17.48 kDa with a theoretical pI of 5.61 and predicted no signal peptide. Real-time polymerase chain reaction analysis revealed that AveIF-5A gene expression was induced by cold, salt and drought stress. To determine the biological function of this gene, recombinant plasmids expressing AveIF-5A and AveIF-5A1 (86-156 aa truncated polypeptide) were used to transform Escherichia coli. The analysis of the growth curves of recombinant E. coli revealed that AveIF-5A and AveIF-5A1 improved the resistance of transformed E. coli to low-temperature, drought and salt stress. These results could provide experimental evidence of the function of the AveIF-5A gene as a valuable gene resource in plant resistance-breeding.</description><subject>Amino acids</subject><subject>Apocynum</subject><subject>Apocynum venetum</subject><subject>Cloning</subject><subject>Drought</subject><subject>E coli</subject><subject>eIF-5A</subject><subject>Gene expression</subject><subject>Growth curves</subject><subject>Low temperature</subject><subject>Low temperature resistance</subject><subject>Molecular weight</subject><subject>Plant breeding</subject><subject>Plant resistance</subject><subject>Plasmids</subject><subject>Polymerase chain reaction</subject><subject>Polypeptides</subject><subject>prokaryotic expression</subject><subject>RACE</subject><subject>real-time PCR</subject><subject>Stress</subject><issn>1310-2818</issn><issn>1314-3530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><sourceid>DOA</sourceid><recordid>eNp9UMtOwzAQjBBIQOETkCJxTlm_YudGqXhUQuICZ8u1N5Wr1C52CvTvSVvgyGl3RzOzu1MUVwTGBBTcEEaAKqLGFEg9JkTShvOj4mzAecUEg-N9D9WOdFqc57wEkABEnhV30y4GHxalCa7Er3XCnH0Mw2i6bfa5jG2Js4dKTMoFBix9KCfraLdhsyo_BqDfrC6Kk9Z0GS9_6qh4e7h_nT5Vzy-Ps-nkubIceF_xGimdD9dIbNxcKW5Ew1rbzC3WjXLcUuRYKymYVQypdbIRcym4JG2tBhobFbODr4tmqdfJr0za6mi83gMxLbRJvbcdat5Aja4W3LTAgRpjQQhnLSPoUO69rg9e6xTfN5h7vYybNPycNaW0qTmXnA4scWDZFHNO2P5tJaB30evf6PUuev0T_aC7Peh8aGNamc-YOqd7s-1iapMJ1mfN_rf4BufFiJ8</recordid><startdate>20160703</startdate><enddate>20160703</enddate><creator>Wang, Lei</creator><creator>Huang, Guo-Qing</creator><creator>Sun, Yao</creator><creator>Li, Yao</creator><creator>Yao, Wen-Jing</creator><creator>Jiang, Ting-Bo</creator><general>Taylor & Francis</general><general>Taylor & Francis Ltd</general><general>Taylor & Francis Group</general><scope>0YH</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7ST</scope><scope>7XB</scope><scope>8FD</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>M2O</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>SOI</scope><scope>DOA</scope></search><sort><creationdate>20160703</creationdate><title>Cloning and expression analysis of eIF-5A gene in Apocynum venetum</title><author>Wang, Lei ; Huang, Guo-Qing ; Sun, Yao ; Li, Yao ; Yao, Wen-Jing ; Jiang, Ting-Bo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-46e22b5307e9db884a593fc9bce698d4c2e4e68753c83e2cd795b75471f68c9b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Amino acids</topic><topic>Apocynum</topic><topic>Apocynum venetum</topic><topic>Cloning</topic><topic>Drought</topic><topic>E coli</topic><topic>eIF-5A</topic><topic>Gene expression</topic><topic>Growth curves</topic><topic>Low temperature</topic><topic>Low temperature resistance</topic><topic>Molecular weight</topic><topic>Plant breeding</topic><topic>Plant resistance</topic><topic>Plasmids</topic><topic>Polymerase chain reaction</topic><topic>Polypeptides</topic><topic>prokaryotic expression</topic><topic>RACE</topic><topic>real-time PCR</topic><topic>Stress</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Lei</creatorcontrib><creatorcontrib>Huang, Guo-Qing</creatorcontrib><creatorcontrib>Sun, Yao</creatorcontrib><creatorcontrib>Li, Yao</creatorcontrib><creatorcontrib>Yao, Wen-Jing</creatorcontrib><creatorcontrib>Jiang, Ting-Bo</creatorcontrib><collection>Taylor & Francis Open Access</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Technology Research Database</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Environment Abstracts</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Biotechnology, biotechnological equipment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Lei</au><au>Huang, Guo-Qing</au><au>Sun, Yao</au><au>Li, Yao</au><au>Yao, Wen-Jing</au><au>Jiang, Ting-Bo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and expression analysis of eIF-5A gene in Apocynum venetum</atitle><jtitle>Biotechnology, biotechnological equipment</jtitle><date>2016-07-03</date><risdate>2016</risdate><volume>30</volume><issue>4</issue><spage>677</spage><epage>684</epage><pages>677-684</pages><issn>1310-2818</issn><eissn>1314-3530</eissn><abstract>To understand the effects of the eIF-5A gene in response to abiotic stress, the 909 bp full-length cDNA of eIF-5A, including a 480 bp open reading frame encoding 159 amino-acid (aa) residues, was isolated from the leaf of herbal plant (Apocynum venetum) by rapid-amplification of cDNA ends. The deduced molecular weight of the encoding protein was 17.48 kDa with a theoretical pI of 5.61 and predicted no signal peptide. Real-time polymerase chain reaction analysis revealed that AveIF-5A gene expression was induced by cold, salt and drought stress. To determine the biological function of this gene, recombinant plasmids expressing AveIF-5A and AveIF-5A1 (86-156 aa truncated polypeptide) were used to transform Escherichia coli. The analysis of the growth curves of recombinant E. coli revealed that AveIF-5A and AveIF-5A1 improved the resistance of transformed E. coli to low-temperature, drought and salt stress. These results could provide experimental evidence of the function of the AveIF-5A gene as a valuable gene resource in plant resistance-breeding.</abstract><cop>Sofia</cop><pub>Taylor & Francis</pub><doi>10.1080/13102818.2016.1172944</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino acids Apocynum Apocynum venetum Cloning Drought E coli eIF-5A Gene expression Growth curves Low temperature Low temperature resistance Molecular weight Plant breeding Plant resistance Plasmids Polymerase chain reaction Polypeptides prokaryotic expression RACE real-time PCR Stress |
| title | Cloning and expression analysis of eIF-5A gene in Apocynum venetum |
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