Preserving extracellular vesicles for biomedical applications: consideration of storage stability before and after isolation
Extracellular vesicles (EVs) are nanovesicles released by various cell types. EVs are known for cell-to-cell communications and have potent biological activities. Despite great progress in recent years for studies exploring the potentials of EVs for early disease detection, therapeutic application a...
Gespeichert in:
| Veröffentlicht in: | Drug delivery 2021-01, Vol.28 (1), p.1501-1509 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1509 |
|---|---|
| container_issue | 1 |
| container_start_page | 1501 |
| container_title | Drug delivery |
| container_volume | 28 |
| creator | Yuan, Fumin Li, Ya-Min Wang, Zhuhui |
| description | Extracellular vesicles (EVs) are nanovesicles released by various cell types. EVs are known for cell-to-cell communications and have potent biological activities. Despite great progress in recent years for studies exploring the potentials of EVs for early disease detection, therapeutic application and drug delivery, determination of the favorable storage conditions of EVs has been challenging. The understanding of the impact of storage conditions on EVs before and after isolation is still limited. Storage may change the size, number, contents, functions, and behaviors of EVs. Here, we summarized current studies about the stability of EVs in different conditions, focusing on temperatures, durations, and freezing and thawing cycles. -80 °C seems to remain the most favorable condition for storage of biofluids and isolated EVs, while isolated EVs may be stored at 4 °C shortly. Lyophilization is promising for storage of EV products. Challenges remain in the understanding of storage-mediated change in EVs and in the development of advanced preservation techniques of EVs. |
| doi_str_mv | 10.1080/10717544.2021.1951896 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1080_10717544_2021_1951896</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_f03ee68a077140769d2bd5824951005e</doaj_id><sourcerecordid>2551580562</sourcerecordid><originalsourceid>FETCH-LOGICAL-c562t-845a7a8ff8938c79d1fe26d3609c4a4a944ec3b758a788c6359ac6acbae7e99d3</originalsourceid><addsrcrecordid>eNp9kkuPFCEUhStG44yjP0FD4sZNtUBBAS7MmImPSSbRha7JLepWS4cuWqhu7cQfL9XdM3FcuOH5ceAeTlU9Z3TBqKavGVVMSSEWnHK2YEYybdoH1TmTnNVUtOJhGRemnqGz6knOK0qpZlw-rs4awaWhRp5Xv78kzJh2flwS_DUlcBjCNkAiO8zeBcxkiIl0Pq6x9w4Cgc0mlMHk45jfEFda32M6zEkcSJ5igiWWHjof_LQnHRYFJDD2BIYJE_E5hgP_tHo0QMj47NRfVN8-vP969am--fzx-urdTe1ky6daCwkK9DBo02inTM8G5G3ftNQ4AQKMEOiaTkkNSmvXNtKAa8F1gAqN6ZuL6vqo20dY2U3ya0h7G8Hbw0JMSwtpmqu1A20QWw1UKSaoak3Pu15qLorBlEosWm-PWpttVyxxOBbTwj3R-zuj_26XcWc114yapgi8Ogmk-GOLebJrn2fXYcS4zZZLyaSmpfKCvvwHXcVtGotVlreGMcG00oWSR8qlmHPC4e4xjNo5K_Y2K3bOij1lpZx78Xcld6duw1GAyyPgx_KDa_gZU-jtBPsQ05BgdD7b5v93_AEhPdDk</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2691141878</pqid></control><display><type>article</type><title>Preserving extracellular vesicles for biomedical applications: consideration of storage stability before and after isolation</title><source>Taylor & Francis Open Access</source><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Yuan, Fumin ; Li, Ya-Min ; Wang, Zhuhui</creator><creatorcontrib>Yuan, Fumin ; Li, Ya-Min ; Wang, Zhuhui</creatorcontrib><description>Extracellular vesicles (EVs) are nanovesicles released by various cell types. EVs are known for cell-to-cell communications and have potent biological activities. Despite great progress in recent years for studies exploring the potentials of EVs for early disease detection, therapeutic application and drug delivery, determination of the favorable storage conditions of EVs has been challenging. The understanding of the impact of storage conditions on EVs before and after isolation is still limited. Storage may change the size, number, contents, functions, and behaviors of EVs. Here, we summarized current studies about the stability of EVs in different conditions, focusing on temperatures, durations, and freezing and thawing cycles. -80 °C seems to remain the most favorable condition for storage of biofluids and isolated EVs, while isolated EVs may be stored at 4 °C shortly. Lyophilization is promising for storage of EV products. Challenges remain in the understanding of storage-mediated change in EVs and in the development of advanced preservation techniques of EVs.</description><identifier>ISSN: 1071-7544</identifier><identifier>EISSN: 1521-0464</identifier><identifier>DOI: 10.1080/10717544.2021.1951896</identifier><identifier>PMID: 34259095</identifier><language>eng</language><publisher>England: Taylor & Francis</publisher><subject>Biobanks ; Biomarkers ; Blood - metabolism ; Blood platelets ; Cell culture ; Cell Culture Techniques ; Disease ; Drug Delivery Systems ; Drug Stability ; exosomes ; Extracellular vesicles ; Extracellular Vesicles - chemistry ; Extracellular Vesicles - metabolism ; Freeze Drying ; Medical equipment ; Milk, Human - metabolism ; nanomedicine ; preservation ; Semen - metabolism ; storage ; Temperature ; Urine - cytology</subject><ispartof>Drug delivery, 2021-01, Vol.28 (1), p.1501-1509</ispartof><rights>2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. 2021</rights><rights>2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This work is licensed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. 2021 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c562t-845a7a8ff8938c79d1fe26d3609c4a4a944ec3b758a788c6359ac6acbae7e99d3</citedby><cites>FETCH-LOGICAL-c562t-845a7a8ff8938c79d1fe26d3609c4a4a944ec3b758a788c6359ac6acbae7e99d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281093/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281093/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,862,883,2098,27489,27911,27912,53778,53780,59128,59129</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34259095$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yuan, Fumin</creatorcontrib><creatorcontrib>Li, Ya-Min</creatorcontrib><creatorcontrib>Wang, Zhuhui</creatorcontrib><title>Preserving extracellular vesicles for biomedical applications: consideration of storage stability before and after isolation</title><title>Drug delivery</title><addtitle>Drug Deliv</addtitle><description>Extracellular vesicles (EVs) are nanovesicles released by various cell types. EVs are known for cell-to-cell communications and have potent biological activities. Despite great progress in recent years for studies exploring the potentials of EVs for early disease detection, therapeutic application and drug delivery, determination of the favorable storage conditions of EVs has been challenging. The understanding of the impact of storage conditions on EVs before and after isolation is still limited. Storage may change the size, number, contents, functions, and behaviors of EVs. Here, we summarized current studies about the stability of EVs in different conditions, focusing on temperatures, durations, and freezing and thawing cycles. -80 °C seems to remain the most favorable condition for storage of biofluids and isolated EVs, while isolated EVs may be stored at 4 °C shortly. Lyophilization is promising for storage of EV products. Challenges remain in the understanding of storage-mediated change in EVs and in the development of advanced preservation techniques of EVs.</description><subject>Biobanks</subject><subject>Biomarkers</subject><subject>Blood - metabolism</subject><subject>Blood platelets</subject><subject>Cell culture</subject><subject>Cell Culture Techniques</subject><subject>Disease</subject><subject>Drug Delivery Systems</subject><subject>Drug Stability</subject><subject>exosomes</subject><subject>Extracellular vesicles</subject><subject>Extracellular Vesicles - chemistry</subject><subject>Extracellular Vesicles - metabolism</subject><subject>Freeze Drying</subject><subject>Medical equipment</subject><subject>Milk, Human - metabolism</subject><subject>nanomedicine</subject><subject>preservation</subject><subject>Semen - metabolism</subject><subject>storage</subject><subject>Temperature</subject><subject>Urine - cytology</subject><issn>1071-7544</issn><issn>1521-0464</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNp9kkuPFCEUhStG44yjP0FD4sZNtUBBAS7MmImPSSbRha7JLepWS4cuWqhu7cQfL9XdM3FcuOH5ceAeTlU9Z3TBqKavGVVMSSEWnHK2YEYybdoH1TmTnNVUtOJhGRemnqGz6knOK0qpZlw-rs4awaWhRp5Xv78kzJh2flwS_DUlcBjCNkAiO8zeBcxkiIl0Pq6x9w4Cgc0mlMHk45jfEFda32M6zEkcSJ5igiWWHjof_LQnHRYFJDD2BIYJE_E5hgP_tHo0QMj47NRfVN8-vP969am--fzx-urdTe1ky6daCwkK9DBo02inTM8G5G3ftNQ4AQKMEOiaTkkNSmvXNtKAa8F1gAqN6ZuL6vqo20dY2U3ya0h7G8Hbw0JMSwtpmqu1A20QWw1UKSaoak3Pu15qLorBlEosWm-PWpttVyxxOBbTwj3R-zuj_26XcWc114yapgi8Ogmk-GOLebJrn2fXYcS4zZZLyaSmpfKCvvwHXcVtGotVlreGMcG00oWSR8qlmHPC4e4xjNo5K_Y2K3bOij1lpZx78Xcld6duw1GAyyPgx_KDa_gZU-jtBPsQ05BgdD7b5v93_AEhPdDk</recordid><startdate>20210101</startdate><enddate>20210101</enddate><creator>Yuan, Fumin</creator><creator>Li, Ya-Min</creator><creator>Wang, Zhuhui</creator><general>Taylor & Francis</general><general>Taylor & Francis Ltd</general><general>Taylor & Francis Group</general><scope>0YH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88I</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M2P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20210101</creationdate><title>Preserving extracellular vesicles for biomedical applications: consideration of storage stability before and after isolation</title><author>Yuan, Fumin ; Li, Ya-Min ; Wang, Zhuhui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c562t-845a7a8ff8938c79d1fe26d3609c4a4a944ec3b758a788c6359ac6acbae7e99d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biobanks</topic><topic>Biomarkers</topic><topic>Blood - metabolism</topic><topic>Blood platelets</topic><topic>Cell culture</topic><topic>Cell Culture Techniques</topic><topic>Disease</topic><topic>Drug Delivery Systems</topic><topic>Drug Stability</topic><topic>exosomes</topic><topic>Extracellular vesicles</topic><topic>Extracellular Vesicles - chemistry</topic><topic>Extracellular Vesicles - metabolism</topic><topic>Freeze Drying</topic><topic>Medical equipment</topic><topic>Milk, Human - metabolism</topic><topic>nanomedicine</topic><topic>preservation</topic><topic>Semen - metabolism</topic><topic>storage</topic><topic>Temperature</topic><topic>Urine - cytology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yuan, Fumin</creatorcontrib><creatorcontrib>Li, Ya-Min</creatorcontrib><creatorcontrib>Wang, Zhuhui</creatorcontrib><collection>Taylor & Francis Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Science Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Drug delivery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yuan, Fumin</au><au>Li, Ya-Min</au><au>Wang, Zhuhui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preserving extracellular vesicles for biomedical applications: consideration of storage stability before and after isolation</atitle><jtitle>Drug delivery</jtitle><addtitle>Drug Deliv</addtitle><date>2021-01-01</date><risdate>2021</risdate><volume>28</volume><issue>1</issue><spage>1501</spage><epage>1509</epage><pages>1501-1509</pages><issn>1071-7544</issn><eissn>1521-0464</eissn><abstract>Extracellular vesicles (EVs) are nanovesicles released by various cell types. EVs are known for cell-to-cell communications and have potent biological activities. Despite great progress in recent years for studies exploring the potentials of EVs for early disease detection, therapeutic application and drug delivery, determination of the favorable storage conditions of EVs has been challenging. The understanding of the impact of storage conditions on EVs before and after isolation is still limited. Storage may change the size, number, contents, functions, and behaviors of EVs. Here, we summarized current studies about the stability of EVs in different conditions, focusing on temperatures, durations, and freezing and thawing cycles. -80 °C seems to remain the most favorable condition for storage of biofluids and isolated EVs, while isolated EVs may be stored at 4 °C shortly. Lyophilization is promising for storage of EV products. Challenges remain in the understanding of storage-mediated change in EVs and in the development of advanced preservation techniques of EVs.</abstract><cop>England</cop><pub>Taylor & Francis</pub><pmid>34259095</pmid><doi>10.1080/10717544.2021.1951896</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1071-7544 |
| ispartof | Drug delivery, 2021-01, Vol.28 (1), p.1501-1509 |
| issn | 1071-7544 1521-0464 |
| language | eng |
| recordid | cdi_crossref_primary_10_1080_10717544_2021_1951896 |
| source | Taylor & Francis Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Biobanks Biomarkers Blood - metabolism Blood platelets Cell culture Cell Culture Techniques Disease Drug Delivery Systems Drug Stability exosomes Extracellular vesicles Extracellular Vesicles - chemistry Extracellular Vesicles - metabolism Freeze Drying Medical equipment Milk, Human - metabolism nanomedicine preservation Semen - metabolism storage Temperature Urine - cytology |
| title | Preserving extracellular vesicles for biomedical applications: consideration of storage stability before and after isolation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T20%3A58%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Preserving%20extracellular%20vesicles%20for%20biomedical%20applications:%20consideration%20of%20storage%20stability%20before%20and%20after%20isolation&rft.jtitle=Drug%20delivery&rft.au=Yuan,%20Fumin&rft.date=2021-01-01&rft.volume=28&rft.issue=1&rft.spage=1501&rft.epage=1509&rft.pages=1501-1509&rft.issn=1071-7544&rft.eissn=1521-0464&rft_id=info:doi/10.1080/10717544.2021.1951896&rft_dat=%3Cproquest_cross%3E2551580562%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2691141878&rft_id=info:pmid/34259095&rft_doaj_id=oai_doaj_org_article_f03ee68a077140769d2bd5824951005e&rfr_iscdi=true |