Effective Treatment of Bladder Tumor-Bearing Mice by Direct Delivery of Bleomycin Using Electrochemotherapy
We investigated the cytotoxicity on the combination of bleomycin (BLM) with electric pulses against transplanted bladder tumors in nude mice. Loading with high-voltage electric pulse after injection of BLM was associated with a decrease in tumor size; tumor size became smaller and disappeared 8 days...
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| Veröffentlicht in: | Drug delivery 2002-10, Vol.9 (4), p.249-252 |
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| creator | Mitsui, Kenji Kato, Keitaro Nakamura, Kogenta Yamada, Yoshiaki Honda, Nobuaki Fukatsu, Hidetoshi Yoshikawa, Kazuhiro |
| description | We investigated the cytotoxicity on the combination of bleomycin (BLM) with electric pulses against transplanted bladder tumors in nude mice. Loading with high-voltage electric pulse after injection of BLM was associated with a decrease in tumor size; tumor size became smaller and disappeared 8 days after the treatment. Such complete regression was achieved with one treatment using BLM of one-tenth the lethal dose (LD 50) combined with electric pulses. On day 20, regrowth was observed at a much slower rate than at lower concentrations of BLM. Pathological examinations of the tumor tissues after treatment revealed disruption of the nucleus in the exposed tissues on day 2. Furthermore, a decrease in number of the stained nuclei and cytoplasmic lysis were observed on day 4. These results showed that electric pulses was an effective tool to deliver drugs into bladder tumor cells and an effective treatment method for bladder tumors. |
| doi_str_mv | 10.1080/10717540260397864 |
| format | Article |
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Loading with high-voltage electric pulse after injection of BLM was associated with a decrease in tumor size; tumor size became smaller and disappeared 8 days after the treatment. Such complete regression was achieved with one treatment using BLM of one-tenth the lethal dose (LD 50) combined with electric pulses. On day 20, regrowth was observed at a much slower rate than at lower concentrations of BLM. Pathological examinations of the tumor tissues after treatment revealed disruption of the nucleus in the exposed tissues on day 2. Furthermore, a decrease in number of the stained nuclei and cytoplasmic lysis were observed on day 4. These results showed that electric pulses was an effective tool to deliver drugs into bladder tumor cells and an effective treatment method for bladder tumors.</description><identifier>ISSN: 0049-8254</identifier><identifier>ISSN: 1071-7544</identifier><identifier>EISSN: 1366-5928</identifier><identifier>EISSN: 1521-0464</identifier><identifier>DOI: 10.1080/10717540260397864</identifier><identifier>PMID: 12511203</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>Animals ; Bladder Cancer ; Bleomycin ; Bleomycin - administration & dosage ; Drug Delivery Systems - methods ; Electric Stimulation - methods ; Electrochemistry ; Female ; High-Voltage Electric Pulse ; Humans ; Mice ; Mice, Nude ; Urinary Bladder Neoplasms - drug therapy ; Urinary Bladder Neoplasms - therapy ; Xenograft Model Antitumor Assays - methods</subject><ispartof>Drug delivery, 2002-10, Vol.9 (4), p.249-252</ispartof><rights>2002 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-79f2a3c0b7c220c0fe3a02079159cdf43cdaa37e0531461b81e8c6d88ddde4113</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/10717540260397864$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/10717540260397864$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,59647,60436,61221,61256,61402,61437</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12511203$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mitsui, Kenji</creatorcontrib><creatorcontrib>Kato, Keitaro</creatorcontrib><creatorcontrib>Nakamura, Kogenta</creatorcontrib><creatorcontrib>Yamada, Yoshiaki</creatorcontrib><creatorcontrib>Honda, Nobuaki</creatorcontrib><creatorcontrib>Fukatsu, Hidetoshi</creatorcontrib><creatorcontrib>Yoshikawa, Kazuhiro</creatorcontrib><title>Effective Treatment of Bladder Tumor-Bearing Mice by Direct Delivery of Bleomycin Using Electrochemotherapy</title><title>Drug delivery</title><addtitle>Drug Deliv</addtitle><description>We investigated the cytotoxicity on the combination of bleomycin (BLM) with electric pulses against transplanted bladder tumors in nude mice. Loading with high-voltage electric pulse after injection of BLM was associated with a decrease in tumor size; tumor size became smaller and disappeared 8 days after the treatment. Such complete regression was achieved with one treatment using BLM of one-tenth the lethal dose (LD 50) combined with electric pulses. On day 20, regrowth was observed at a much slower rate than at lower concentrations of BLM. Pathological examinations of the tumor tissues after treatment revealed disruption of the nucleus in the exposed tissues on day 2. Furthermore, a decrease in number of the stained nuclei and cytoplasmic lysis were observed on day 4. These results showed that electric pulses was an effective tool to deliver drugs into bladder tumor cells and an effective treatment method for bladder tumors.</description><subject>Animals</subject><subject>Bladder Cancer</subject><subject>Bleomycin</subject><subject>Bleomycin - administration & dosage</subject><subject>Drug Delivery Systems - methods</subject><subject>Electric Stimulation - methods</subject><subject>Electrochemistry</subject><subject>Female</subject><subject>High-Voltage Electric Pulse</subject><subject>Humans</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Urinary Bladder Neoplasms - drug therapy</subject><subject>Urinary Bladder Neoplasms - therapy</subject><subject>Xenograft Model Antitumor Assays - methods</subject><issn>0049-8254</issn><issn>1071-7544</issn><issn>1366-5928</issn><issn>1521-0464</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAURS0EokPLD2CDsmIXeP5I7Ag2tB2gUlE307XlcZ6ZlCQenpOi_HtczUgIIbWrtznn6uo-xt5weM_BwAcOmutKgahBNtrU6hlbcVnXZdUI85ytAFRTGlGpE_YqpTsAqLkQL9kJFxXnAuSK_VyHgH7q7rHYELppwHEqYijOe9e2SMVmHiKV5-ioG38U3zuPxXYpLjvKUnGJfRZpOQgYh8V3Y3GbHtB1nwmKfodDnHZIbr-csRfB9QlfH-8pu_2y3lx8K69vvl5dfL4uvWqaqdRNEE562GovBHgIKB0I0A2vGt8GJX3rnNQIleSq5lvD0fi6NabNjRXn8pS9O-TuKf6aMU126JLHvncjxjlZLQxoodSTINfG5A2rDPID6CmmRBjsnrrB0WI52IdX2P9ekZ23x_B5O2D71zhun4FPB6AbQ6TB_Y7Ut3ZySx8pkBt9l6x8LP_jP_oOXT_tvCO0d3GmMS_8SLs_ahOpgA</recordid><startdate>20021001</startdate><enddate>20021001</enddate><creator>Mitsui, Kenji</creator><creator>Kato, Keitaro</creator><creator>Nakamura, Kogenta</creator><creator>Yamada, Yoshiaki</creator><creator>Honda, Nobuaki</creator><creator>Fukatsu, Hidetoshi</creator><creator>Yoshikawa, Kazuhiro</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20021001</creationdate><title>Effective Treatment of Bladder Tumor-Bearing Mice by Direct Delivery of Bleomycin Using Electrochemotherapy</title><author>Mitsui, Kenji ; Kato, Keitaro ; Nakamura, Kogenta ; Yamada, Yoshiaki ; Honda, Nobuaki ; Fukatsu, Hidetoshi ; Yoshikawa, Kazuhiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-79f2a3c0b7c220c0fe3a02079159cdf43cdaa37e0531461b81e8c6d88ddde4113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Bladder Cancer</topic><topic>Bleomycin</topic><topic>Bleomycin - administration & dosage</topic><topic>Drug Delivery Systems - methods</topic><topic>Electric Stimulation - methods</topic><topic>Electrochemistry</topic><topic>Female</topic><topic>High-Voltage Electric Pulse</topic><topic>Humans</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Urinary Bladder Neoplasms - drug therapy</topic><topic>Urinary Bladder Neoplasms - therapy</topic><topic>Xenograft Model Antitumor Assays - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mitsui, Kenji</creatorcontrib><creatorcontrib>Kato, Keitaro</creatorcontrib><creatorcontrib>Nakamura, Kogenta</creatorcontrib><creatorcontrib>Yamada, Yoshiaki</creatorcontrib><creatorcontrib>Honda, Nobuaki</creatorcontrib><creatorcontrib>Fukatsu, Hidetoshi</creatorcontrib><creatorcontrib>Yoshikawa, Kazuhiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Drug delivery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mitsui, Kenji</au><au>Kato, Keitaro</au><au>Nakamura, Kogenta</au><au>Yamada, Yoshiaki</au><au>Honda, Nobuaki</au><au>Fukatsu, Hidetoshi</au><au>Yoshikawa, Kazuhiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effective Treatment of Bladder Tumor-Bearing Mice by Direct Delivery of Bleomycin Using Electrochemotherapy</atitle><jtitle>Drug delivery</jtitle><addtitle>Drug Deliv</addtitle><date>2002-10-01</date><risdate>2002</risdate><volume>9</volume><issue>4</issue><spage>249</spage><epage>252</epage><pages>249-252</pages><issn>0049-8254</issn><issn>1071-7544</issn><eissn>1366-5928</eissn><eissn>1521-0464</eissn><abstract>We investigated the cytotoxicity on the combination of bleomycin (BLM) with electric pulses against transplanted bladder tumors in nude mice. Loading with high-voltage electric pulse after injection of BLM was associated with a decrease in tumor size; tumor size became smaller and disappeared 8 days after the treatment. Such complete regression was achieved with one treatment using BLM of one-tenth the lethal dose (LD 50) combined with electric pulses. On day 20, regrowth was observed at a much slower rate than at lower concentrations of BLM. Pathological examinations of the tumor tissues after treatment revealed disruption of the nucleus in the exposed tissues on day 2. Furthermore, a decrease in number of the stained nuclei and cytoplasmic lysis were observed on day 4. These results showed that electric pulses was an effective tool to deliver drugs into bladder tumor cells and an effective treatment method for bladder tumors.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>12511203</pmid><doi>10.1080/10717540260397864</doi><tpages>4</tpages></addata></record> |
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| source | MEDLINE; Taylor & Francis Medical Library - CRKN; Access via Taylor & Francis; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Bladder Cancer Bleomycin Bleomycin - administration & dosage Drug Delivery Systems - methods Electric Stimulation - methods Electrochemistry Female High-Voltage Electric Pulse Humans Mice Mice, Nude Urinary Bladder Neoplasms - drug therapy Urinary Bladder Neoplasms - therapy Xenograft Model Antitumor Assays - methods |
| title | Effective Treatment of Bladder Tumor-Bearing Mice by Direct Delivery of Bleomycin Using Electrochemotherapy |
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