Quantitative Assessment of Protein-bound Tyrosine Nitration in Airway Secretions from Patients with Inflammatory Airway Disease
Because reactive nitrogen species (RNS) have potent inflammatory activity, they may be involved in the inflammatory process in pulmonary diseases. We recently reported increased numbers of 3-nitrotyrosine immunopositive cells, which are evidences of RNS production, in the sputum of patients with chr...
Gespeichert in:
Veröffentlicht in: | Free radical research 2004-01, Vol.38 (1), p.49-57 |
---|---|
Hauptverfasser: | , , , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 57 |
---|---|
container_issue | 1 |
container_start_page | 49 |
container_title | Free radical research |
container_volume | 38 |
creator | Sugiura, Hisatoshi Ichinose, Masakazu Tomaki, Masafumi Ogawa, Hiromasa Koarai, Akira Kitamuro, Tomomi Komaki, Yuichi Akita, Takefumi Nishino, Hirohito Okamoto, Shinichiro Akaike, Takaaki Hattori, Toshio |
description | Because reactive nitrogen species (RNS) have potent inflammatory activity, they may be involved in the inflammatory process in pulmonary diseases. We recently reported increased numbers of 3-nitrotyrosine immunopositive cells, which are evidences of RNS production, in the sputum of patients with chronic obstructive pulmonary disease (COPD) and patients with asthma compared with healthy subjects. In the present study, we attempted to quantify this protein nitration in the airways by means of high-performance liquid chromatography (HPLC) used together with an electrochemical detection system that we developed. Sputum samples were obtained from 15 stable COPD patients, 9 asthmatic patients and 7 healthy subjects by using hypertonic saline inhalation. The values for the molar ratio of protein-bound 3-nitrotyrosine/tyrosine in patients with asthma (4.31±1.13 × 10-6, p |
doi_str_mv | 10.1080/10715760310001633817 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1080_10715760310001633817</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>71793493</sourcerecordid><originalsourceid>FETCH-LOGICAL-c414t-4678a4c5d28a25655f9d4fa5dcd868217770897d0036a538a53a6aa4623cb17f3</originalsourceid><addsrcrecordid>eNqFkF1rFDEUhoMo9kP_gUiuvJuaTL5mbpSlfhWKbbFeh7OZhE2ZSWqScZmr_nWz7IoURC8O5xCe9z05L0KvKDmjpCNvKVFUKEkYJYRQyVhH1RN0TEnbNy1X5OluVrSpTHuETnK-qxjjQj1HR1QQSaVgx-jhZoZQfIHif1q8ytnmPNlQcHT4OsVifWjWcQ4Dvl1SzD5Y_NWXVPEYsA945dMWFvzNmmR3bxm7FCd8XYHqkvHWlw2-CG6EaYIS0_Jb8cFnC9m-QM8cjNm-PPRT9P3Tx9vzL83l1eeL89VlYzjlpeFSdcCNGNoOWiGFcP3AHYjBDJ3sWqqUIl2vBkKYBMG6WiABuGyZWVPl2Cl6s_e9T_HHbHPRk8_GjiMEG-esFVU94z2rIN-Dpp6bk3X6PvkJ0qIp0bvg9d-Cr7LXB_95Pdnhj-iQdAXe7wEfXEwTbGMaB11gGWNyCYLxWbP_rHj3yGFjYSwbA8nquzinUOP79x9_ASgdpkQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71793493</pqid></control><display><type>article</type><title>Quantitative Assessment of Protein-bound Tyrosine Nitration in Airway Secretions from Patients with Inflammatory Airway Disease</title><source>MEDLINE</source><source>Taylor & Francis:Master (3349 titles)</source><creator>Sugiura, Hisatoshi ; Ichinose, Masakazu ; Tomaki, Masafumi ; Ogawa, Hiromasa ; Koarai, Akira ; Kitamuro, Tomomi ; Komaki, Yuichi ; Akita, Takefumi ; Nishino, Hirohito ; Okamoto, Shinichiro ; Akaike, Takaaki ; Hattori, Toshio</creator><creatorcontrib>Sugiura, Hisatoshi ; Ichinose, Masakazu ; Tomaki, Masafumi ; Ogawa, Hiromasa ; Koarai, Akira ; Kitamuro, Tomomi ; Komaki, Yuichi ; Akita, Takefumi ; Nishino, Hirohito ; Okamoto, Shinichiro ; Akaike, Takaaki ; Hattori, Toshio</creatorcontrib><description>Because reactive nitrogen species (RNS) have potent inflammatory activity, they may be involved in the inflammatory process in pulmonary diseases. We recently reported increased numbers of 3-nitrotyrosine immunopositive cells, which are evidences of RNS production, in the sputum of patients with chronic obstructive pulmonary disease (COPD) and patients with asthma compared with healthy subjects. In the present study, we attempted to quantify this protein nitration in the airways by means of high-performance liquid chromatography (HPLC) used together with an electrochemical detection system that we developed. Sputum samples were obtained from 15 stable COPD patients, 9 asthmatic patients and 7 healthy subjects by using hypertonic saline inhalation. The values for the molar ratio of protein-bound 3-nitrotyrosine/tyrosine in patients with asthma (4.31±1.13 × 10-6, p<0.05) and patients with COPD (3.04±0.36 × 10-6, p<0.01) were significantly higher than those in healthy subjects (1.37±0.19 × 10-6). The levels of protein-bound 3-nitrotyrosine in the airways were not significantly different in asthmatic patients and COPD patients. A significant negative correlation was found between values for protein-bound 3-nitrotyrosine/tyrosine and % FEV1 values in patients with COPD (r=-0.53, p<0.05) but not in patients with asthma. These results suggest that our HPLC-electrochemical method is useful for quantifying RNS production in human airways. More importantly, they show that increased RNS production in the airways seems to contribute in a critical way to the pathogenesis of COPD, and that the effects of RNS in airways may differ in asthma and COPD.</description><identifier>ISSN: 1071-5762</identifier><identifier>EISSN: 1029-2470</identifier><identifier>DOI: 10.1080/10715760310001633817</identifier><identifier>PMID: 15061653</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>3-Nitrotyrosine ; Aged ; Asthma ; Asthma - metabolism ; Asthma - physiopathology ; Case-Control Studies ; Chromatography, High Pressure Liquid - methods ; COPD ; Electrochemistry - methods ; Female ; Forced Expiratory Volume ; Humans ; Male ; Middle Aged ; Nitric Oxide - analysis ; Proteins - metabolism ; Pulmonary Disease, Chronic Obstructive - metabolism ; Pulmonary Disease, Chronic Obstructive - physiopathology ; Reactive nitrogen species ; Reference Values ; Sensitivity and Specificity ; Sputum - metabolism ; Tyrosine - analogs & derivatives ; Tyrosine - analysis ; Tyrosine - metabolism</subject><ispartof>Free radical research, 2004-01, Vol.38 (1), p.49-57</ispartof><rights>2004 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c414t-4678a4c5d28a25655f9d4fa5dcd868217770897d0036a538a53a6aa4623cb17f3</citedby><cites>FETCH-LOGICAL-c414t-4678a4c5d28a25655f9d4fa5dcd868217770897d0036a538a53a6aa4623cb17f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/10715760310001633817$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/10715760310001633817$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,59647,60436,61221,61402</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15061653$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sugiura, Hisatoshi</creatorcontrib><creatorcontrib>Ichinose, Masakazu</creatorcontrib><creatorcontrib>Tomaki, Masafumi</creatorcontrib><creatorcontrib>Ogawa, Hiromasa</creatorcontrib><creatorcontrib>Koarai, Akira</creatorcontrib><creatorcontrib>Kitamuro, Tomomi</creatorcontrib><creatorcontrib>Komaki, Yuichi</creatorcontrib><creatorcontrib>Akita, Takefumi</creatorcontrib><creatorcontrib>Nishino, Hirohito</creatorcontrib><creatorcontrib>Okamoto, Shinichiro</creatorcontrib><creatorcontrib>Akaike, Takaaki</creatorcontrib><creatorcontrib>Hattori, Toshio</creatorcontrib><title>Quantitative Assessment of Protein-bound Tyrosine Nitration in Airway Secretions from Patients with Inflammatory Airway Disease</title><title>Free radical research</title><addtitle>Free Radic Res</addtitle><description>Because reactive nitrogen species (RNS) have potent inflammatory activity, they may be involved in the inflammatory process in pulmonary diseases. We recently reported increased numbers of 3-nitrotyrosine immunopositive cells, which are evidences of RNS production, in the sputum of patients with chronic obstructive pulmonary disease (COPD) and patients with asthma compared with healthy subjects. In the present study, we attempted to quantify this protein nitration in the airways by means of high-performance liquid chromatography (HPLC) used together with an electrochemical detection system that we developed. Sputum samples were obtained from 15 stable COPD patients, 9 asthmatic patients and 7 healthy subjects by using hypertonic saline inhalation. The values for the molar ratio of protein-bound 3-nitrotyrosine/tyrosine in patients with asthma (4.31±1.13 × 10-6, p<0.05) and patients with COPD (3.04±0.36 × 10-6, p<0.01) were significantly higher than those in healthy subjects (1.37±0.19 × 10-6). The levels of protein-bound 3-nitrotyrosine in the airways were not significantly different in asthmatic patients and COPD patients. A significant negative correlation was found between values for protein-bound 3-nitrotyrosine/tyrosine and % FEV1 values in patients with COPD (r=-0.53, p<0.05) but not in patients with asthma. These results suggest that our HPLC-electrochemical method is useful for quantifying RNS production in human airways. More importantly, they show that increased RNS production in the airways seems to contribute in a critical way to the pathogenesis of COPD, and that the effects of RNS in airways may differ in asthma and COPD.</description><subject>3-Nitrotyrosine</subject><subject>Aged</subject><subject>Asthma</subject><subject>Asthma - metabolism</subject><subject>Asthma - physiopathology</subject><subject>Case-Control Studies</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>COPD</subject><subject>Electrochemistry - methods</subject><subject>Female</subject><subject>Forced Expiratory Volume</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Nitric Oxide - analysis</subject><subject>Proteins - metabolism</subject><subject>Pulmonary Disease, Chronic Obstructive - metabolism</subject><subject>Pulmonary Disease, Chronic Obstructive - physiopathology</subject><subject>Reactive nitrogen species</subject><subject>Reference Values</subject><subject>Sensitivity and Specificity</subject><subject>Sputum - metabolism</subject><subject>Tyrosine - analogs & derivatives</subject><subject>Tyrosine - analysis</subject><subject>Tyrosine - metabolism</subject><issn>1071-5762</issn><issn>1029-2470</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkF1rFDEUhoMo9kP_gUiuvJuaTL5mbpSlfhWKbbFeh7OZhE2ZSWqScZmr_nWz7IoURC8O5xCe9z05L0KvKDmjpCNvKVFUKEkYJYRQyVhH1RN0TEnbNy1X5OluVrSpTHuETnK-qxjjQj1HR1QQSaVgx-jhZoZQfIHif1q8ytnmPNlQcHT4OsVifWjWcQ4Dvl1SzD5Y_NWXVPEYsA945dMWFvzNmmR3bxm7FCd8XYHqkvHWlw2-CG6EaYIS0_Jb8cFnC9m-QM8cjNm-PPRT9P3Tx9vzL83l1eeL89VlYzjlpeFSdcCNGNoOWiGFcP3AHYjBDJ3sWqqUIl2vBkKYBMG6WiABuGyZWVPl2Cl6s_e9T_HHbHPRk8_GjiMEG-esFVU94z2rIN-Dpp6bk3X6PvkJ0qIp0bvg9d-Cr7LXB_95Pdnhj-iQdAXe7wEfXEwTbGMaB11gGWNyCYLxWbP_rHj3yGFjYSwbA8nquzinUOP79x9_ASgdpkQ</recordid><startdate>200401</startdate><enddate>200401</enddate><creator>Sugiura, Hisatoshi</creator><creator>Ichinose, Masakazu</creator><creator>Tomaki, Masafumi</creator><creator>Ogawa, Hiromasa</creator><creator>Koarai, Akira</creator><creator>Kitamuro, Tomomi</creator><creator>Komaki, Yuichi</creator><creator>Akita, Takefumi</creator><creator>Nishino, Hirohito</creator><creator>Okamoto, Shinichiro</creator><creator>Akaike, Takaaki</creator><creator>Hattori, Toshio</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200401</creationdate><title>Quantitative Assessment of Protein-bound Tyrosine Nitration in Airway Secretions from Patients with Inflammatory Airway Disease</title><author>Sugiura, Hisatoshi ; Ichinose, Masakazu ; Tomaki, Masafumi ; Ogawa, Hiromasa ; Koarai, Akira ; Kitamuro, Tomomi ; Komaki, Yuichi ; Akita, Takefumi ; Nishino, Hirohito ; Okamoto, Shinichiro ; Akaike, Takaaki ; Hattori, Toshio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c414t-4678a4c5d28a25655f9d4fa5dcd868217770897d0036a538a53a6aa4623cb17f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>3-Nitrotyrosine</topic><topic>Aged</topic><topic>Asthma</topic><topic>Asthma - metabolism</topic><topic>Asthma - physiopathology</topic><topic>Case-Control Studies</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>COPD</topic><topic>Electrochemistry - methods</topic><topic>Female</topic><topic>Forced Expiratory Volume</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Nitric Oxide - analysis</topic><topic>Proteins - metabolism</topic><topic>Pulmonary Disease, Chronic Obstructive - metabolism</topic><topic>Pulmonary Disease, Chronic Obstructive - physiopathology</topic><topic>Reactive nitrogen species</topic><topic>Reference Values</topic><topic>Sensitivity and Specificity</topic><topic>Sputum - metabolism</topic><topic>Tyrosine - analogs & derivatives</topic><topic>Tyrosine - analysis</topic><topic>Tyrosine - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sugiura, Hisatoshi</creatorcontrib><creatorcontrib>Ichinose, Masakazu</creatorcontrib><creatorcontrib>Tomaki, Masafumi</creatorcontrib><creatorcontrib>Ogawa, Hiromasa</creatorcontrib><creatorcontrib>Koarai, Akira</creatorcontrib><creatorcontrib>Kitamuro, Tomomi</creatorcontrib><creatorcontrib>Komaki, Yuichi</creatorcontrib><creatorcontrib>Akita, Takefumi</creatorcontrib><creatorcontrib>Nishino, Hirohito</creatorcontrib><creatorcontrib>Okamoto, Shinichiro</creatorcontrib><creatorcontrib>Akaike, Takaaki</creatorcontrib><creatorcontrib>Hattori, Toshio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Free radical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sugiura, Hisatoshi</au><au>Ichinose, Masakazu</au><au>Tomaki, Masafumi</au><au>Ogawa, Hiromasa</au><au>Koarai, Akira</au><au>Kitamuro, Tomomi</au><au>Komaki, Yuichi</au><au>Akita, Takefumi</au><au>Nishino, Hirohito</au><au>Okamoto, Shinichiro</au><au>Akaike, Takaaki</au><au>Hattori, Toshio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative Assessment of Protein-bound Tyrosine Nitration in Airway Secretions from Patients with Inflammatory Airway Disease</atitle><jtitle>Free radical research</jtitle><addtitle>Free Radic Res</addtitle><date>2004-01</date><risdate>2004</risdate><volume>38</volume><issue>1</issue><spage>49</spage><epage>57</epage><pages>49-57</pages><issn>1071-5762</issn><eissn>1029-2470</eissn><abstract>Because reactive nitrogen species (RNS) have potent inflammatory activity, they may be involved in the inflammatory process in pulmonary diseases. We recently reported increased numbers of 3-nitrotyrosine immunopositive cells, which are evidences of RNS production, in the sputum of patients with chronic obstructive pulmonary disease (COPD) and patients with asthma compared with healthy subjects. In the present study, we attempted to quantify this protein nitration in the airways by means of high-performance liquid chromatography (HPLC) used together with an electrochemical detection system that we developed. Sputum samples were obtained from 15 stable COPD patients, 9 asthmatic patients and 7 healthy subjects by using hypertonic saline inhalation. The values for the molar ratio of protein-bound 3-nitrotyrosine/tyrosine in patients with asthma (4.31±1.13 × 10-6, p<0.05) and patients with COPD (3.04±0.36 × 10-6, p<0.01) were significantly higher than those in healthy subjects (1.37±0.19 × 10-6). The levels of protein-bound 3-nitrotyrosine in the airways were not significantly different in asthmatic patients and COPD patients. A significant negative correlation was found between values for protein-bound 3-nitrotyrosine/tyrosine and % FEV1 values in patients with COPD (r=-0.53, p<0.05) but not in patients with asthma. These results suggest that our HPLC-electrochemical method is useful for quantifying RNS production in human airways. More importantly, they show that increased RNS production in the airways seems to contribute in a critical way to the pathogenesis of COPD, and that the effects of RNS in airways may differ in asthma and COPD.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>15061653</pmid><doi>10.1080/10715760310001633817</doi><tpages>9</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1071-5762 |
ispartof | Free radical research, 2004-01, Vol.38 (1), p.49-57 |
issn | 1071-5762 1029-2470 |
language | eng |
recordid | cdi_crossref_primary_10_1080_10715760310001633817 |
source | MEDLINE; Taylor & Francis:Master (3349 titles) |
subjects | 3-Nitrotyrosine Aged Asthma Asthma - metabolism Asthma - physiopathology Case-Control Studies Chromatography, High Pressure Liquid - methods COPD Electrochemistry - methods Female Forced Expiratory Volume Humans Male Middle Aged Nitric Oxide - analysis Proteins - metabolism Pulmonary Disease, Chronic Obstructive - metabolism Pulmonary Disease, Chronic Obstructive - physiopathology Reactive nitrogen species Reference Values Sensitivity and Specificity Sputum - metabolism Tyrosine - analogs & derivatives Tyrosine - analysis Tyrosine - metabolism |
title | Quantitative Assessment of Protein-bound Tyrosine Nitration in Airway Secretions from Patients with Inflammatory Airway Disease |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T15%3A59%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Quantitative%20Assessment%20of%20Protein-bound%20Tyrosine%20Nitration%20in%20Airway%20Secretions%20from%20Patients%20with%20Inflammatory%20Airway%20Disease&rft.jtitle=Free%20radical%20research&rft.au=Sugiura,%20Hisatoshi&rft.date=2004-01&rft.volume=38&rft.issue=1&rft.spage=49&rft.epage=57&rft.pages=49-57&rft.issn=1071-5762&rft.eissn=1029-2470&rft_id=info:doi/10.1080/10715760310001633817&rft_dat=%3Cproquest_cross%3E71793493%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71793493&rft_id=info:pmid/15061653&rfr_iscdi=true |