Intracellular pH regulation in ventral horn neurones cultured from embryonic rat spinal cord

Intracellular pH was measured with the pH-sensitive fluorescent probe BCECF in spinal cord neurones cultured from rat embryos. At an external pH of 7.3, the average steady-state pHi was 7.18 +/- 0.03 (SEM, n = 97) and 7.02 +/- 0.01 (n = 221) in HEPES-buffered and in bicarbonate-buffered medium, resp...

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Veröffentlicht in:	Molecular membrane biology 2000, Vol.17 (2), p.101-108
1. Verfasser:	Rodeau, Caroline Brechenmacher, Jean-Luc
Format:	Artikel
Sprache:	eng
Schlagworte:	4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid - pharmacology Acid-Base Equilibrium - physiology Amiloride - pharmacology Ammonium Chloride - pharmacology Animals Anterior Horn Cells - embryology Anterior Horn Cells - metabolism Antiporters - metabolism Buffers Cerebrospinal Fluid - chemistry Culture Media - chemistry Embryo, Mammalian - metabolism Fluoresceins - pharmacology Fluorescent Dyes - pharmacology Hydrogen-Ion Concentration Intracellular Ph Spinal Cord Bcecf Rats Sodium Bicarbonate - metabolism
Online-Zugang:	Volltext
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