Intracellular pH regulation in ventral horn neurones cultured from embryonic rat spinal cord
Intracellular pH was measured with the pH-sensitive fluorescent probe BCECF in spinal cord neurones cultured from rat embryos. At an external pH of 7.3, the average steady-state pHi was 7.18 +/- 0.03 (SEM, n = 97) and 7.02 +/- 0.01 (n = 221) in HEPES-buffered and in bicarbonate-buffered medium, resp...
Gespeichert in:
| Veröffentlicht in: | Molecular membrane biology 2000, Vol.17 (2), p.101-108 |
|---|---|
| 1. Verfasser: | |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 108 |
|---|---|
| container_issue | 2 |
| container_start_page | 101 |
| container_title | Molecular membrane biology |
| container_volume | 17 |
| creator | Rodeau, Caroline Brechenmacher, Jean-Luc |
| description | Intracellular pH was measured with the pH-sensitive fluorescent probe BCECF in spinal cord neurones cultured from rat embryos. At an external pH of 7.3, the average steady-state pHi was 7.18 +/- 0.03 (SEM, n = 97) and 7.02 +/- 0.01 (n = 221) in HEPES-buffered and in bicarbonate-buffered medium, respectively. In both external media, pHi was strongly dependent on external pH (pHe). In HEPES-buffered medium, pHi recovery following an acid load induced by transient application of ammonium required external Na+ and was inhibited by amiloride, indicating the presence of a Na+/H+ exchange. Na(+)- and HCO3(-)-dependent, DIDS-sensitive alkalinizing mechanisms also contributed to pHi regulation in CO2/bicarbonate-buffered medium. The presence of an electrogenic Na(+)-HCO3- cotransporter was confirmed by the alkalinizing effect of KCl application. The fact that pHi is lower in CO2/bicarbonate- than in HEPES-buffered medium and the alkalinization observed upon suppression of external Cl- suggest that the acidifying Cl-/HCO3- transporter plays an important role in defining pHi. |
| doi_str_mv | 10.1080/09687680050117129 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1080_09687680050117129</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>72257593</sourcerecordid><originalsourceid>FETCH-LOGICAL-c445t-ac5e54a9dcb2411cb5c4d5e3e3b4580ffd369c02b17e2c73045b811e0c14cbb53</originalsourceid><addsrcrecordid>eNp9kE9LJDEQxYMoOqt-AC-Sk7deK91J_8G9LKKrIHjRmxCSdLXTkk7GSvfKfHt7GA-K4KkK3u89qh5jJwJ-C6jhHJqyrsoaQIEQlcibHbYQspSZyqHYZYuNns1AfcB-pfQCALIs5T47ENDUjSxhwZ5uw0jGofeTN8RXN5zweV7HPgbeB_4fN7rny0iBB5woBkzcTX6cCFveURw4DpbWMfSOkxl5WvVhNrhI7RHb64xPePwxD9nj9dXD5U12d__v9vLvXeakVGNmnEIlTdM6m0shnFVOtgoLLKxUNXRdW5SNg9yKCnNXFSCVrYVAcEI6a1VxyM62uSuKrxOmUQ992vxkAsYp6SrPVaWaYgbFFnQUUyLs9Ir6wdBaC9CbSvW3SmfP6Uf4ZAdsPzm2Hc7Any3Qhy7SYN4i-VaPZu0jdWSC65Mufsq_-GJfovHj0hlC_RInmrtMP1z3DhJsmDI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72257593</pqid></control><display><type>article</type><title>Intracellular pH regulation in ventral horn neurones cultured from embryonic rat spinal cord</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Taylor & Francis Journals Complete</source><creator>Rodeau, Caroline Brechenmacher, Jean-Luc</creator><creatorcontrib>Rodeau, Caroline Brechenmacher, Jean-Luc</creatorcontrib><description>Intracellular pH was measured with the pH-sensitive fluorescent probe BCECF in spinal cord neurones cultured from rat embryos. At an external pH of 7.3, the average steady-state pHi was 7.18 +/- 0.03 (SEM, n = 97) and 7.02 +/- 0.01 (n = 221) in HEPES-buffered and in bicarbonate-buffered medium, respectively. In both external media, pHi was strongly dependent on external pH (pHe). In HEPES-buffered medium, pHi recovery following an acid load induced by transient application of ammonium required external Na+ and was inhibited by amiloride, indicating the presence of a Na+/H+ exchange. Na(+)- and HCO3(-)-dependent, DIDS-sensitive alkalinizing mechanisms also contributed to pHi regulation in CO2/bicarbonate-buffered medium. The presence of an electrogenic Na(+)-HCO3- cotransporter was confirmed by the alkalinizing effect of KCl application. The fact that pHi is lower in CO2/bicarbonate- than in HEPES-buffered medium and the alkalinization observed upon suppression of external Cl- suggest that the acidifying Cl-/HCO3- transporter plays an important role in defining pHi.</description><identifier>ISSN: 0968-7688</identifier><identifier>EISSN: 1464-5203</identifier><identifier>DOI: 10.1080/09687680050117129</identifier><identifier>PMID: 10989460</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid - pharmacology ; Acid-Base Equilibrium - physiology ; Amiloride - pharmacology ; Ammonium Chloride - pharmacology ; Animals ; Anterior Horn Cells - embryology ; Anterior Horn Cells - metabolism ; Antiporters - metabolism ; Buffers ; Cerebrospinal Fluid - chemistry ; Culture Media - chemistry ; Embryo, Mammalian - metabolism ; Fluoresceins - pharmacology ; Fluorescent Dyes - pharmacology ; Hydrogen-Ion Concentration ; Intracellular Ph Spinal Cord Bcecf ; Rats ; Sodium Bicarbonate - metabolism</subject><ispartof>Molecular membrane biology, 2000, Vol.17 (2), p.101-108</ispartof><rights>2000 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2000</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-ac5e54a9dcb2411cb5c4d5e3e3b4580ffd369c02b17e2c73045b811e0c14cbb53</citedby><cites>FETCH-LOGICAL-c445t-ac5e54a9dcb2411cb5c4d5e3e3b4580ffd369c02b17e2c73045b811e0c14cbb53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/09687680050117129$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/09687680050117129$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>314,776,780,4009,27902,27903,27904,59623,60412,61197,61378</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10989460$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rodeau, Caroline Brechenmacher, Jean-Luc</creatorcontrib><title>Intracellular pH regulation in ventral horn neurones cultured from embryonic rat spinal cord</title><title>Molecular membrane biology</title><addtitle>Mol Membr Biol</addtitle><description>Intracellular pH was measured with the pH-sensitive fluorescent probe BCECF in spinal cord neurones cultured from rat embryos. At an external pH of 7.3, the average steady-state pHi was 7.18 +/- 0.03 (SEM, n = 97) and 7.02 +/- 0.01 (n = 221) in HEPES-buffered and in bicarbonate-buffered medium, respectively. In both external media, pHi was strongly dependent on external pH (pHe). In HEPES-buffered medium, pHi recovery following an acid load induced by transient application of ammonium required external Na+ and was inhibited by amiloride, indicating the presence of a Na+/H+ exchange. Na(+)- and HCO3(-)-dependent, DIDS-sensitive alkalinizing mechanisms also contributed to pHi regulation in CO2/bicarbonate-buffered medium. The presence of an electrogenic Na(+)-HCO3- cotransporter was confirmed by the alkalinizing effect of KCl application. The fact that pHi is lower in CO2/bicarbonate- than in HEPES-buffered medium and the alkalinization observed upon suppression of external Cl- suggest that the acidifying Cl-/HCO3- transporter plays an important role in defining pHi.</description><subject>4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid - pharmacology</subject><subject>Acid-Base Equilibrium - physiology</subject><subject>Amiloride - pharmacology</subject><subject>Ammonium Chloride - pharmacology</subject><subject>Animals</subject><subject>Anterior Horn Cells - embryology</subject><subject>Anterior Horn Cells - metabolism</subject><subject>Antiporters - metabolism</subject><subject>Buffers</subject><subject>Cerebrospinal Fluid - chemistry</subject><subject>Culture Media - chemistry</subject><subject>Embryo, Mammalian - metabolism</subject><subject>Fluoresceins - pharmacology</subject><subject>Fluorescent Dyes - pharmacology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Intracellular Ph Spinal Cord Bcecf</subject><subject>Rats</subject><subject>Sodium Bicarbonate - metabolism</subject><issn>0968-7688</issn><issn>1464-5203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9LJDEQxYMoOqt-AC-Sk7deK91J_8G9LKKrIHjRmxCSdLXTkk7GSvfKfHt7GA-K4KkK3u89qh5jJwJ-C6jhHJqyrsoaQIEQlcibHbYQspSZyqHYZYuNns1AfcB-pfQCALIs5T47ENDUjSxhwZ5uw0jGofeTN8RXN5zweV7HPgbeB_4fN7rny0iBB5woBkzcTX6cCFveURw4DpbWMfSOkxl5WvVhNrhI7RHb64xPePwxD9nj9dXD5U12d__v9vLvXeakVGNmnEIlTdM6m0shnFVOtgoLLKxUNXRdW5SNg9yKCnNXFSCVrYVAcEI6a1VxyM62uSuKrxOmUQ992vxkAsYp6SrPVaWaYgbFFnQUUyLs9Ir6wdBaC9CbSvW3SmfP6Uf4ZAdsPzm2Hc7Any3Qhy7SYN4i-VaPZu0jdWSC65Mufsq_-GJfovHj0hlC_RInmrtMP1z3DhJsmDI</recordid><startdate>2000</startdate><enddate>2000</enddate><creator>Rodeau, Caroline Brechenmacher, Jean-Luc</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2000</creationdate><title>Intracellular pH regulation in ventral horn neurones cultured from embryonic rat spinal cord</title><author>Rodeau, Caroline Brechenmacher, Jean-Luc</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-ac5e54a9dcb2411cb5c4d5e3e3b4580ffd369c02b17e2c73045b811e0c14cbb53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid - pharmacology</topic><topic>Acid-Base Equilibrium - physiology</topic><topic>Amiloride - pharmacology</topic><topic>Ammonium Chloride - pharmacology</topic><topic>Animals</topic><topic>Anterior Horn Cells - embryology</topic><topic>Anterior Horn Cells - metabolism</topic><topic>Antiporters - metabolism</topic><topic>Buffers</topic><topic>Cerebrospinal Fluid - chemistry</topic><topic>Culture Media - chemistry</topic><topic>Embryo, Mammalian - metabolism</topic><topic>Fluoresceins - pharmacology</topic><topic>Fluorescent Dyes - pharmacology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Intracellular Ph Spinal Cord Bcecf</topic><topic>Rats</topic><topic>Sodium Bicarbonate - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rodeau, Caroline Brechenmacher, Jean-Luc</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular membrane biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rodeau, Caroline Brechenmacher, Jean-Luc</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intracellular pH regulation in ventral horn neurones cultured from embryonic rat spinal cord</atitle><jtitle>Molecular membrane biology</jtitle><addtitle>Mol Membr Biol</addtitle><date>2000</date><risdate>2000</risdate><volume>17</volume><issue>2</issue><spage>101</spage><epage>108</epage><pages>101-108</pages><issn>0968-7688</issn><eissn>1464-5203</eissn><abstract>Intracellular pH was measured with the pH-sensitive fluorescent probe BCECF in spinal cord neurones cultured from rat embryos. At an external pH of 7.3, the average steady-state pHi was 7.18 +/- 0.03 (SEM, n = 97) and 7.02 +/- 0.01 (n = 221) in HEPES-buffered and in bicarbonate-buffered medium, respectively. In both external media, pHi was strongly dependent on external pH (pHe). In HEPES-buffered medium, pHi recovery following an acid load induced by transient application of ammonium required external Na+ and was inhibited by amiloride, indicating the presence of a Na+/H+ exchange. Na(+)- and HCO3(-)-dependent, DIDS-sensitive alkalinizing mechanisms also contributed to pHi regulation in CO2/bicarbonate-buffered medium. The presence of an electrogenic Na(+)-HCO3- cotransporter was confirmed by the alkalinizing effect of KCl application. The fact that pHi is lower in CO2/bicarbonate- than in HEPES-buffered medium and the alkalinization observed upon suppression of external Cl- suggest that the acidifying Cl-/HCO3- transporter plays an important role in defining pHi.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>10989460</pmid><doi>10.1080/09687680050117129</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0968-7688 |
| ispartof | Molecular membrane biology, 2000, Vol.17 (2), p.101-108 |
| issn | 0968-7688 1464-5203 |
| language | eng |
| recordid | cdi_crossref_primary_10_1080_09687680050117129 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Taylor & Francis Journals Complete |
| subjects | 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid - pharmacology Acid-Base Equilibrium - physiology Amiloride - pharmacology Ammonium Chloride - pharmacology Animals Anterior Horn Cells - embryology Anterior Horn Cells - metabolism Antiporters - metabolism Buffers Cerebrospinal Fluid - chemistry Culture Media - chemistry Embryo, Mammalian - metabolism Fluoresceins - pharmacology Fluorescent Dyes - pharmacology Hydrogen-Ion Concentration Intracellular Ph Spinal Cord Bcecf Rats Sodium Bicarbonate - metabolism |
| title | Intracellular pH regulation in ventral horn neurones cultured from embryonic rat spinal cord |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-26T12%3A16%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Intracellular%20pH%20regulation%20in%20ventral%20horn%20neurones%20cultured%20from%20embryonic%20rat%20spinal%20cord&rft.jtitle=Molecular%20membrane%20biology&rft.au=Rodeau,%20Caroline%20Brechenmacher,%20Jean-Luc&rft.date=2000&rft.volume=17&rft.issue=2&rft.spage=101&rft.epage=108&rft.pages=101-108&rft.issn=0968-7688&rft.eissn=1464-5203&rft_id=info:doi/10.1080/09687680050117129&rft_dat=%3Cproquest_cross%3E72257593%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=72257593&rft_id=info:pmid/10989460&rfr_iscdi=true |