RNA-seq based transcriptome analysis of murine macrophages RAW264.7 treated with multi-herbal extracts reveals immunomodulatory potential and mechanism
Shenling Baizhu Powder is a traditional Chinese medicine formula that has been used for thousands of years to invigorate spleen, nourish stomach and regulate immune function. The multi-herbal extracts, an improved formular from Shenling Baizhu Powder, were used in our study to investigate its immune...
Gespeichert in:
| Veröffentlicht in: | Food and agricultural immunology 2023-12, Vol.34 (1), p.21-35 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 35 |
|---|---|
| container_issue | 1 |
| container_start_page | 21 |
| container_title | Food and agricultural immunology |
| container_volume | 34 |
| creator | Jin, Meng-Yuan Chen, Xin Wu, Qin Guo, Zhi-Jian Zhang, Rui Jiang, Yi-Fan Li, Xiang Zhang, Li-da Wang, Yu-Liang Zhou, Xuan-Wei |
| description | Shenling Baizhu Powder is a traditional Chinese medicine formula that has been used for thousands of years to invigorate spleen, nourish stomach and regulate immune function. The multi-herbal extracts, an improved formular from Shenling Baizhu Powder, were used in our study to investigate its immune-enhancing effects and underling mechanisms. Based on UPLC-Q-TOF-MS and SPME-GC-MS analysis, 106 chemical compounds were identified from the extracts. In vitro assays indicated that the extracts could promote cell proliferation and NO secretion of RAW264.7 cells. RNA-seq and RT-qPCR showed that RAW264.7 cells treated with extracts increased the expression of pro-inflammatory factors IL-1β, iNOS, TNF-α, and TGF-β. Western-Blot revealed that the extracts could significantly enhance ERK phosphorylation level, and slightly increased JNK and p38 phosphorylation levels. These data suggest that multi-herbal extracts have a potential of regulating immune response via the JNK/p38/ERK signalling of MAPK pathways in RAW264.7 cells. |
| doi_str_mv | 10.1080/09540105.2023.2192443 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1080_09540105_2023_2192443</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_e6a6fe349a1a4abd9e8aab6115867869</doaj_id><sourcerecordid>2895598533</sourcerecordid><originalsourceid>FETCH-LOGICAL-c399t-2bbd769e0f124edfd7a8cb345fcef645b486f359ea48121267c2798154820bbe3</originalsourceid><addsrcrecordid>eNp9kV1rFDEUhgdRcK3-BCHg9az5nuTOpfhRKApF8TKcmTnpZpmZbJOsdX-Jf7fZbvXSqxzC8z4Hzts0bxldM2roe2qVpIyqNadcrDmzXErxrFkxqVUr6vy8WZ2Y9gS9bF7lvKOUSm3Vqvlz83XTZrwjPWQcSUmw5CGFfYkzElhgOuaQSfRkPqSwIJlhSHG_hVvM5Gbzk2u57moKodT0fSjbCk4ltFtMPUwEf1fjUDJJ-AthyiTM82GJcxwPE5SYjmQfCy4lVBaWkcw4bGEJeX7dvPCVxzdP70Xz49PH75df2utvn68uN9ftIKwtLe_7sdMWqWdc4ujHDszQC6n8gF5L1UujvVAWQRrGGdfdwDtrmJKG075HcdFcnb1jhJ3bpzBDOroIwT1-xHTrIJUwTOhQg_YopAUGEvrRogHoNWPK6M5oW13vzq59incHzMXt4iHVG2bHjVXKGiVEpdSZqofMOaH_t5VRd-rT_e3Tnfp0T33W3IdzLiw-phnuY5pGV-A4xeRrbUPITvxf8QCzO6mO</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2895598533</pqid></control><display><type>article</type><title>RNA-seq based transcriptome analysis of murine macrophages RAW264.7 treated with multi-herbal extracts reveals immunomodulatory potential and mechanism</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Access via Taylor & Francis (Open Access Collection)</source><creator>Jin, Meng-Yuan ; Chen, Xin ; Wu, Qin ; Guo, Zhi-Jian ; Zhang, Rui ; Jiang, Yi-Fan ; Li, Xiang ; Zhang, Li-da ; Wang, Yu-Liang ; Zhou, Xuan-Wei</creator><creatorcontrib>Jin, Meng-Yuan ; Chen, Xin ; Wu, Qin ; Guo, Zhi-Jian ; Zhang, Rui ; Jiang, Yi-Fan ; Li, Xiang ; Zhang, Li-da ; Wang, Yu-Liang ; Zhou, Xuan-Wei</creatorcontrib><description>Shenling Baizhu Powder is a traditional Chinese medicine formula that has been used for thousands of years to invigorate spleen, nourish stomach and regulate immune function. The multi-herbal extracts, an improved formular from Shenling Baizhu Powder, were used in our study to investigate its immune-enhancing effects and underling mechanisms. Based on UPLC-Q-TOF-MS and SPME-GC-MS analysis, 106 chemical compounds were identified from the extracts. In vitro assays indicated that the extracts could promote cell proliferation and NO secretion of RAW264.7 cells. RNA-seq and RT-qPCR showed that RAW264.7 cells treated with extracts increased the expression of pro-inflammatory factors IL-1β, iNOS, TNF-α, and TGF-β. Western-Blot revealed that the extracts could significantly enhance ERK phosphorylation level, and slightly increased JNK and p38 phosphorylation levels. These data suggest that multi-herbal extracts have a potential of regulating immune response via the JNK/p38/ERK signalling of MAPK pathways in RAW264.7 cells.</description><identifier>ISSN: 0954-0105</identifier><identifier>EISSN: 1465-3443</identifier><identifier>DOI: 10.1080/09540105.2023.2192443</identifier><language>eng</language><publisher>Abingdon: Taylor & Francis</publisher><subject>Active compounds ; Cell proliferation ; Chemical compounds ; Herbal medicine ; Immune response ; Immune system ; Immunomodulation ; immunomodulatory activity ; Inflammation ; Macrophages ; MAP kinase ; multi-herbal extracts ; Nitric-oxide synthase ; Phosphorylation ; Plant extracts ; RNA-seq ; Signal transduction ; signalling pathways ; Solid phase methods ; Traditional Chinese medicine ; Transcriptomes ; Transforming growth factor-b ; Tumor necrosis factor-α</subject><ispartof>Food and agricultural immunology, 2023-12, Vol.34 (1), p.21-35</ispartof><rights>2023 The Author(s). Published with license by Taylor & Francis Group, LLC. 2023</rights><rights>2023 The Author(s). Published with license by Taylor & Francis Group, LLC. This work is licensed under the Creative Commons Attribution – Non-Commercial – No Derivatives License http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c399t-2bbd769e0f124edfd7a8cb345fcef645b486f359ea48121267c2798154820bbe3</cites><orcidid>0000-0002-3993-6346</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/09540105.2023.2192443$$EPDF$$P50$$Ginformaworld$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/09540105.2023.2192443$$EHTML$$P50$$Ginformaworld$$Hfree_for_read</linktohtml><link.rule.ids>315,782,786,866,2104,27509,27931,27932,59150,59151</link.rule.ids></links><search><creatorcontrib>Jin, Meng-Yuan</creatorcontrib><creatorcontrib>Chen, Xin</creatorcontrib><creatorcontrib>Wu, Qin</creatorcontrib><creatorcontrib>Guo, Zhi-Jian</creatorcontrib><creatorcontrib>Zhang, Rui</creatorcontrib><creatorcontrib>Jiang, Yi-Fan</creatorcontrib><creatorcontrib>Li, Xiang</creatorcontrib><creatorcontrib>Zhang, Li-da</creatorcontrib><creatorcontrib>Wang, Yu-Liang</creatorcontrib><creatorcontrib>Zhou, Xuan-Wei</creatorcontrib><title>RNA-seq based transcriptome analysis of murine macrophages RAW264.7 treated with multi-herbal extracts reveals immunomodulatory potential and mechanism</title><title>Food and agricultural immunology</title><description>Shenling Baizhu Powder is a traditional Chinese medicine formula that has been used for thousands of years to invigorate spleen, nourish stomach and regulate immune function. The multi-herbal extracts, an improved formular from Shenling Baizhu Powder, were used in our study to investigate its immune-enhancing effects and underling mechanisms. Based on UPLC-Q-TOF-MS and SPME-GC-MS analysis, 106 chemical compounds were identified from the extracts. In vitro assays indicated that the extracts could promote cell proliferation and NO secretion of RAW264.7 cells. RNA-seq and RT-qPCR showed that RAW264.7 cells treated with extracts increased the expression of pro-inflammatory factors IL-1β, iNOS, TNF-α, and TGF-β. Western-Blot revealed that the extracts could significantly enhance ERK phosphorylation level, and slightly increased JNK and p38 phosphorylation levels. These data suggest that multi-herbal extracts have a potential of regulating immune response via the JNK/p38/ERK signalling of MAPK pathways in RAW264.7 cells.</description><subject>Active compounds</subject><subject>Cell proliferation</subject><subject>Chemical compounds</subject><subject>Herbal medicine</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Immunomodulation</subject><subject>immunomodulatory activity</subject><subject>Inflammation</subject><subject>Macrophages</subject><subject>MAP kinase</subject><subject>multi-herbal extracts</subject><subject>Nitric-oxide synthase</subject><subject>Phosphorylation</subject><subject>Plant extracts</subject><subject>RNA-seq</subject><subject>Signal transduction</subject><subject>signalling pathways</subject><subject>Solid phase methods</subject><subject>Traditional Chinese medicine</subject><subject>Transcriptomes</subject><subject>Transforming growth factor-b</subject><subject>Tumor necrosis factor-α</subject><issn>0954-0105</issn><issn>1465-3443</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>DOA</sourceid><recordid>eNp9kV1rFDEUhgdRcK3-BCHg9az5nuTOpfhRKApF8TKcmTnpZpmZbJOsdX-Jf7fZbvXSqxzC8z4Hzts0bxldM2roe2qVpIyqNadcrDmzXErxrFkxqVUr6vy8WZ2Y9gS9bF7lvKOUSm3Vqvlz83XTZrwjPWQcSUmw5CGFfYkzElhgOuaQSfRkPqSwIJlhSHG_hVvM5Gbzk2u57moKodT0fSjbCk4ltFtMPUwEf1fjUDJJ-AthyiTM82GJcxwPE5SYjmQfCy4lVBaWkcw4bGEJeX7dvPCVxzdP70Xz49PH75df2utvn68uN9ftIKwtLe_7sdMWqWdc4ujHDszQC6n8gF5L1UujvVAWQRrGGdfdwDtrmJKG075HcdFcnb1jhJ3bpzBDOroIwT1-xHTrIJUwTOhQg_YopAUGEvrRogHoNWPK6M5oW13vzq59incHzMXt4iHVG2bHjVXKGiVEpdSZqofMOaH_t5VRd-rT_e3Tnfp0T33W3IdzLiw-phnuY5pGV-A4xeRrbUPITvxf8QCzO6mO</recordid><startdate>20231231</startdate><enddate>20231231</enddate><creator>Jin, Meng-Yuan</creator><creator>Chen, Xin</creator><creator>Wu, Qin</creator><creator>Guo, Zhi-Jian</creator><creator>Zhang, Rui</creator><creator>Jiang, Yi-Fan</creator><creator>Li, Xiang</creator><creator>Zhang, Li-da</creator><creator>Wang, Yu-Liang</creator><creator>Zhou, Xuan-Wei</creator><general>Taylor & Francis</general><general>Taylor & Francis Ltd</general><general>Taylor & Francis Group</general><scope>0YH</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-3993-6346</orcidid></search><sort><creationdate>20231231</creationdate><title>RNA-seq based transcriptome analysis of murine macrophages RAW264.7 treated with multi-herbal extracts reveals immunomodulatory potential and mechanism</title><author>Jin, Meng-Yuan ; Chen, Xin ; Wu, Qin ; Guo, Zhi-Jian ; Zhang, Rui ; Jiang, Yi-Fan ; Li, Xiang ; Zhang, Li-da ; Wang, Yu-Liang ; Zhou, Xuan-Wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c399t-2bbd769e0f124edfd7a8cb345fcef645b486f359ea48121267c2798154820bbe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Active compounds</topic><topic>Cell proliferation</topic><topic>Chemical compounds</topic><topic>Herbal medicine</topic><topic>Immune response</topic><topic>Immune system</topic><topic>Immunomodulation</topic><topic>immunomodulatory activity</topic><topic>Inflammation</topic><topic>Macrophages</topic><topic>MAP kinase</topic><topic>multi-herbal extracts</topic><topic>Nitric-oxide synthase</topic><topic>Phosphorylation</topic><topic>Plant extracts</topic><topic>RNA-seq</topic><topic>Signal transduction</topic><topic>signalling pathways</topic><topic>Solid phase methods</topic><topic>Traditional Chinese medicine</topic><topic>Transcriptomes</topic><topic>Transforming growth factor-b</topic><topic>Tumor necrosis factor-α</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jin, Meng-Yuan</creatorcontrib><creatorcontrib>Chen, Xin</creatorcontrib><creatorcontrib>Wu, Qin</creatorcontrib><creatorcontrib>Guo, Zhi-Jian</creatorcontrib><creatorcontrib>Zhang, Rui</creatorcontrib><creatorcontrib>Jiang, Yi-Fan</creatorcontrib><creatorcontrib>Li, Xiang</creatorcontrib><creatorcontrib>Zhang, Li-da</creatorcontrib><creatorcontrib>Wang, Yu-Liang</creatorcontrib><creatorcontrib>Zhou, Xuan-Wei</creatorcontrib><collection>Access via Taylor & Francis (Open Access Collection)</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Food and agricultural immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jin, Meng-Yuan</au><au>Chen, Xin</au><au>Wu, Qin</au><au>Guo, Zhi-Jian</au><au>Zhang, Rui</au><au>Jiang, Yi-Fan</au><au>Li, Xiang</au><au>Zhang, Li-da</au><au>Wang, Yu-Liang</au><au>Zhou, Xuan-Wei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RNA-seq based transcriptome analysis of murine macrophages RAW264.7 treated with multi-herbal extracts reveals immunomodulatory potential and mechanism</atitle><jtitle>Food and agricultural immunology</jtitle><date>2023-12-31</date><risdate>2023</risdate><volume>34</volume><issue>1</issue><spage>21</spage><epage>35</epage><pages>21-35</pages><issn>0954-0105</issn><eissn>1465-3443</eissn><abstract>Shenling Baizhu Powder is a traditional Chinese medicine formula that has been used for thousands of years to invigorate spleen, nourish stomach and regulate immune function. The multi-herbal extracts, an improved formular from Shenling Baizhu Powder, were used in our study to investigate its immune-enhancing effects and underling mechanisms. Based on UPLC-Q-TOF-MS and SPME-GC-MS analysis, 106 chemical compounds were identified from the extracts. In vitro assays indicated that the extracts could promote cell proliferation and NO secretion of RAW264.7 cells. RNA-seq and RT-qPCR showed that RAW264.7 cells treated with extracts increased the expression of pro-inflammatory factors IL-1β, iNOS, TNF-α, and TGF-β. Western-Blot revealed that the extracts could significantly enhance ERK phosphorylation level, and slightly increased JNK and p38 phosphorylation levels. These data suggest that multi-herbal extracts have a potential of regulating immune response via the JNK/p38/ERK signalling of MAPK pathways in RAW264.7 cells.</abstract><cop>Abingdon</cop><pub>Taylor & Francis</pub><doi>10.1080/09540105.2023.2192443</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-3993-6346</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0954-0105 |
| ispartof | Food and agricultural immunology, 2023-12, Vol.34 (1), p.21-35 |
| issn | 0954-0105 1465-3443 |
| language | eng |
| recordid | cdi_crossref_primary_10_1080_09540105_2023_2192443 |
| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Access via Taylor & Francis (Open Access Collection) |
| subjects | Active compounds Cell proliferation Chemical compounds Herbal medicine Immune response Immune system Immunomodulation immunomodulatory activity Inflammation Macrophages MAP kinase multi-herbal extracts Nitric-oxide synthase Phosphorylation Plant extracts RNA-seq Signal transduction signalling pathways Solid phase methods Traditional Chinese medicine Transcriptomes Transforming growth factor-b Tumor necrosis factor-α |
| title | RNA-seq based transcriptome analysis of murine macrophages RAW264.7 treated with multi-herbal extracts reveals immunomodulatory potential and mechanism |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-04T18%3A02%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=RNA-seq%20based%20transcriptome%20analysis%20of%20murine%20macrophages%20RAW264.7%20treated%20with%20multi-herbal%20extracts%20reveals%20immunomodulatory%20potential%20and%20mechanism&rft.jtitle=Food%20and%20agricultural%20immunology&rft.au=Jin,%20Meng-Yuan&rft.date=2023-12-31&rft.volume=34&rft.issue=1&rft.spage=21&rft.epage=35&rft.pages=21-35&rft.issn=0954-0105&rft.eissn=1465-3443&rft_id=info:doi/10.1080/09540105.2023.2192443&rft_dat=%3Cproquest_cross%3E2895598533%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2895598533&rft_id=info:pmid/&rft_doaj_id=oai_doaj_org_article_e6a6fe349a1a4abd9e8aab6115867869&rfr_iscdi=true |