Identification and characterization of MicroRNAs in pig liver after the LPS challenge using RNA-seq

Identification and characterization of MicroRNAs in pig liver after the LPS challenge using RNA-seq

This study was conducted to test the expression profiles of miRNAs in the liver in piglets after the LPS challenge using sequencing technology. Twelve barrows were assigned to two groups, including the saline group and the LPS group. Six small RNA (sRNA) libraries were constructed, and novel miRNAs...

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Veröffentlicht in:Food and agricultural immunology 2022-12, Vol.33 (1), p.652-663
Hauptverfasser: Yang, Yang, Lv, Qingqing, Huang, Xingfa, Fan, Jiajun, Li, Pei, Zhu, Huling, Kang, Ping, Liu, Yulan
Format: Artikel
Sprache:eng
Schlagworte:
Gene expression
Hogs
IL-1β
Interleukin 6
Lipid metabolism
Lipids
Lipopolysaccharide
Lipopolysaccharides
Liver
Metabolism
MicroRNAs
miRNA
pig
Tumor necrosis factor-α
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Zusammenfassung:This study was conducted to test the expression profiles of miRNAs in the liver in piglets after the LPS challenge using sequencing technology. Twelve barrows were assigned to two groups, including the saline group and the LPS group. Six small RNA (sRNA) libraries were constructed, and novel miRNAs were identified using mirevo and mirdeep2 software and validated by quantitative real-time PCR (qRT-PCR). The results showed the mRNA expression of IL-1β, IL-6 and TNF-α in the liver increased after the LPS challenge (P 
ISSN:0954-0105
1465-3443
DOI:10.1080/09540105.2022.2109602