Development of a specific monoclonal antibody assay and a rapid testing strip for the detection of apramycin residues in food samples
A specific monoclonal antibody (MAb) against apramycin (AP) was produced and used to develop an indirect competitive enzyme-linked immunosorbent assay (idcELISA) and a rapid testing strip for the detection of AP residues in foods. MAb exhibited negligible cross-reactivity with other aminoglycosides....
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| Veröffentlicht in: | Food and agricultural immunology 2017-01, Vol.28 (1), p.49-66 |
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| container_title | Food and agricultural immunology |
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| creator | Isanga, Joel Tochi, Bitange Nipa Mukunzi, Daniel Chen, Yanni Liu, Liqiang Kuang, Hua Xu, Chuanlai |
| description | A specific monoclonal antibody (MAb) against apramycin (AP) was produced and used to develop an indirect competitive enzyme-linked immunosorbent assay (idcELISA) and a rapid testing strip for the detection of AP residues in foods. MAb exhibited negligible cross-reactivity with other aminoglycosides. Under optimized conditions in 0.01 M PBS, the half maximum inhibitory concentration (IC
50
) of MAb was 0.41 ng/ml with a limit of detection (LOD) of 0.15 ng/ml. The ELISA results were obtained within 90 min. The mean recoveries from all the spiked food samples were within the range of 79.02-105.49%, with coefficients of variation in the range of 2.21-11.4%. The strip test results obtained within 5 min had visual LODs in the range 2.5-5 µg/kg (ng/ml) for all food samples tested. Therefore, the developed strip test represents a fast and convenient detection method of AP residues in foods. |
| doi_str_mv | 10.1080/09540105.2016.1202211 |
| format | Article |
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) of MAb was 0.41 ng/ml with a limit of detection (LOD) of 0.15 ng/ml. The ELISA results were obtained within 90 min. The mean recoveries from all the spiked food samples were within the range of 79.02-105.49%, with coefficients of variation in the range of 2.21-11.4%. The strip test results obtained within 5 min had visual LODs in the range 2.5-5 µg/kg (ng/ml) for all food samples tested. Therefore, the developed strip test represents a fast and convenient detection method of AP residues in foods.</description><identifier>ISSN: 0954-0105</identifier><identifier>EISSN: 1465-3443</identifier><identifier>DOI: 10.1080/09540105.2016.1202211</identifier><language>eng</language><publisher>Abingdon: Taylor & Francis</publisher><subject>Aminoglycosides ; Apramycin ; Coefficient of variation ; Cross-reactivity ; Enzyme-linked immunosorbent assay ; Food ; indirect competitive enzyme-linked immunosorbent assay ; Monoclonal antibodies ; monoclonal antibody ; rapid strip test ; Residues ; Strip</subject><ispartof>Food and agricultural immunology, 2017-01, Vol.28 (1), p.49-66</ispartof><rights>2016 Informa UK Limited, trading as Taylor & Francis Group 2016</rights><rights>2016 Informa UK Limited, trading as Taylor & Francis Group</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-ef154de27b2e3182a428160f1bddcd2a62ab98c7c7b3319ba59eddc1471f0efe3</citedby><cites>FETCH-LOGICAL-c418t-ef154de27b2e3182a428160f1bddcd2a62ab98c7c7b3319ba59eddc1471f0efe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids></links><search><creatorcontrib>Isanga, Joel</creatorcontrib><creatorcontrib>Tochi, Bitange Nipa</creatorcontrib><creatorcontrib>Mukunzi, Daniel</creatorcontrib><creatorcontrib>Chen, Yanni</creatorcontrib><creatorcontrib>Liu, Liqiang</creatorcontrib><creatorcontrib>Kuang, Hua</creatorcontrib><creatorcontrib>Xu, Chuanlai</creatorcontrib><title>Development of a specific monoclonal antibody assay and a rapid testing strip for the detection of apramycin residues in food samples</title><title>Food and agricultural immunology</title><description>A specific monoclonal antibody (MAb) against apramycin (AP) was produced and used to develop an indirect competitive enzyme-linked immunosorbent assay (idcELISA) and a rapid testing strip for the detection of AP residues in foods. MAb exhibited negligible cross-reactivity with other aminoglycosides. Under optimized conditions in 0.01 M PBS, the half maximum inhibitory concentration (IC
50
) of MAb was 0.41 ng/ml with a limit of detection (LOD) of 0.15 ng/ml. The ELISA results were obtained within 90 min. The mean recoveries from all the spiked food samples were within the range of 79.02-105.49%, with coefficients of variation in the range of 2.21-11.4%. The strip test results obtained within 5 min had visual LODs in the range 2.5-5 µg/kg (ng/ml) for all food samples tested. Therefore, the developed strip test represents a fast and convenient detection method of AP residues in foods.</description><subject>Aminoglycosides</subject><subject>Apramycin</subject><subject>Coefficient of variation</subject><subject>Cross-reactivity</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Food</subject><subject>indirect competitive enzyme-linked immunosorbent assay</subject><subject>Monoclonal antibodies</subject><subject>monoclonal antibody</subject><subject>rapid strip test</subject><subject>Residues</subject><subject>Strip</subject><issn>0954-0105</issn><issn>1465-3443</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNp9kc2KFTEQhYMoeB19BCHgxk1fU0n6b6eM4w8MuNF1SCcVzZBO2iRXuQ_ge5v2jhsXbqoK6qtDcQ4hz4EdgU3sFZt7yYD1R85gOAJnnAM8IAeQQ98JKcVDctiZbocekyel3DHG5DD3B_LrLf7AkLYVY6XJUU3LhsY7b-iaYjIhRR2ojtUvyZ6pLkW3Gm0Ds968pRVL9fErLTX7jbqUaf2G1GJFU32KfzS3rNez8ZFmLN6esNA2u5QsLXrdApan5JHToeCz-35Fvry7-Xz9obv99P7j9ZvbzkiYaocOemmRjwtHARPXkk8wMAeLtcZyPXC9zJMZzbgIAfOi-xnbBuQIjqFDcUVeXnS3nL63P6pafTEYgo6YTkXBzGDkchKioS_-Qe_SKTczdooL0fdsZo3qL5TJqZSMTm3ZrzqfFTC1h6P-hqP2cNR9OO3u9eXOx2bZqn-mHKyq-hxSdllH44sS_5f4DQ-ml_Y</recordid><startdate>20170102</startdate><enddate>20170102</enddate><creator>Isanga, Joel</creator><creator>Tochi, Bitange Nipa</creator><creator>Mukunzi, Daniel</creator><creator>Chen, Yanni</creator><creator>Liu, Liqiang</creator><creator>Kuang, Hua</creator><creator>Xu, Chuanlai</creator><general>Taylor & Francis</general><general>Taylor & Francis Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>20170102</creationdate><title>Development of a specific monoclonal antibody assay and a rapid testing strip for the detection of apramycin residues in food samples</title><author>Isanga, Joel ; Tochi, Bitange Nipa ; Mukunzi, Daniel ; Chen, Yanni ; Liu, Liqiang ; Kuang, Hua ; Xu, Chuanlai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-ef154de27b2e3182a428160f1bddcd2a62ab98c7c7b3319ba59eddc1471f0efe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Aminoglycosides</topic><topic>Apramycin</topic><topic>Coefficient of variation</topic><topic>Cross-reactivity</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Food</topic><topic>indirect competitive enzyme-linked immunosorbent assay</topic><topic>Monoclonal antibodies</topic><topic>monoclonal antibody</topic><topic>rapid strip test</topic><topic>Residues</topic><topic>Strip</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Isanga, Joel</creatorcontrib><creatorcontrib>Tochi, Bitange Nipa</creatorcontrib><creatorcontrib>Mukunzi, Daniel</creatorcontrib><creatorcontrib>Chen, Yanni</creatorcontrib><creatorcontrib>Liu, Liqiang</creatorcontrib><creatorcontrib>Kuang, Hua</creatorcontrib><creatorcontrib>Xu, Chuanlai</creatorcontrib><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Food and agricultural immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Isanga, Joel</au><au>Tochi, Bitange Nipa</au><au>Mukunzi, Daniel</au><au>Chen, Yanni</au><au>Liu, Liqiang</au><au>Kuang, Hua</au><au>Xu, Chuanlai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a specific monoclonal antibody assay and a rapid testing strip for the detection of apramycin residues in food samples</atitle><jtitle>Food and agricultural immunology</jtitle><date>2017-01-02</date><risdate>2017</risdate><volume>28</volume><issue>1</issue><spage>49</spage><epage>66</epage><pages>49-66</pages><issn>0954-0105</issn><eissn>1465-3443</eissn><abstract>A specific monoclonal antibody (MAb) against apramycin (AP) was produced and used to develop an indirect competitive enzyme-linked immunosorbent assay (idcELISA) and a rapid testing strip for the detection of AP residues in foods. MAb exhibited negligible cross-reactivity with other aminoglycosides. Under optimized conditions in 0.01 M PBS, the half maximum inhibitory concentration (IC
50
) of MAb was 0.41 ng/ml with a limit of detection (LOD) of 0.15 ng/ml. The ELISA results were obtained within 90 min. The mean recoveries from all the spiked food samples were within the range of 79.02-105.49%, with coefficients of variation in the range of 2.21-11.4%. The strip test results obtained within 5 min had visual LODs in the range 2.5-5 µg/kg (ng/ml) for all food samples tested. Therefore, the developed strip test represents a fast and convenient detection method of AP residues in foods.</abstract><cop>Abingdon</cop><pub>Taylor & Francis</pub><doi>10.1080/09540105.2016.1202211</doi><tpages>18</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Food and agricultural immunology, 2017-01, Vol.28 (1), p.49-66 |
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| source | DOAJ Directory of Open Access Journals |
| subjects | Aminoglycosides Apramycin Coefficient of variation Cross-reactivity Enzyme-linked immunosorbent assay Food indirect competitive enzyme-linked immunosorbent assay Monoclonal antibodies monoclonal antibody rapid strip test Residues Strip |
| title | Development of a specific monoclonal antibody assay and a rapid testing strip for the detection of apramycin residues in food samples |
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