Knockdown of KLF9 promotes the differentiation of both intramuscular and subcutaneous preadipocytes in goat
KLF9 is reported to promote adipocyte differentiation in 3T3-L1 cells and pigs. However, the roles of KLF9 in adipocytes differentiation of goat remain unknown. In this study, the expression profiles of KLF9 were different between subcutaneous and intramuscular preadipocytes of goat during different...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2020-08, Vol.84 (8), p.1594-1602 |
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| description | KLF9 is reported to promote adipocyte differentiation in 3T3-L1 cells and pigs. However, the roles of KLF9 in adipocytes differentiation of goat remain unknown. In this study, the expression profiles of KLF9 were different between subcutaneous and intramuscular preadipocytes of goat during differentiation process. After silencing KLF9 gene, the lipid droplets were increased in both two types of adipocytes. In subcutaneous preadipocyte with silencing KLF9, the expressions of C/EBPβ, PPARγ, LPL, KLF1-2, KLF5, and KLF17 genes were up-regulated, while KLF12, KLF4, and KLF13 genes were down-regulated in expression level. In intramuscular preadipocyte, aP2, C/EBPα, KLF2-3, KLF5, and KLF7 gene were up-regulated, and Pref-1 gene was down-regulated. In addition, the binding sites of KLF9 existed in the promoters of aP2, C/EBPα, C/EBPβ, LPL and Pref-1. Taken together, KLF9 play a negative role in the differentiation of both intramuscular and subcutaneous preadipocytes in goats, but the functional mechanism may be different.
Knockdown KLF9 promotes differentiation of intramuscular and subcutaneous preadipocyte, however, the mechanism of action might be different. |
| doi_str_mv | 10.1080/09168451.2020.1767497 |
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Knockdown KLF9 promotes differentiation of intramuscular and subcutaneous preadipocyte, however, the mechanism of action might be different.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1080/09168451.2020.1767497</identifier><identifier>PMID: 32434447</identifier><language>eng</language><publisher>England: Taylor & Francis</publisher><subject>Adipocytes - cytology ; Adipocytes - metabolism ; Adipogenesis - genetics ; Animals ; Calcium-Binding Proteins - genetics ; Calcium-Binding Proteins - metabolism ; CCAAT-Enhancer-Binding Protein-beta - genetics ; CCAAT-Enhancer-Binding Protein-beta - metabolism ; Cell Differentiation - genetics ; Fatty Acid-Binding Proteins - genetics ; Fatty Acid-Binding Proteins - metabolism ; Gene Expression Regulation ; Goat ; Goats ; intramuscular adipocytes ; KLF9 ; Kruppel-Like Transcription Factors - antagonists & inhibitors ; Kruppel-Like Transcription Factors - genetics ; Kruppel-Like Transcription Factors - metabolism ; Lipoprotein Lipase - genetics ; Lipoprotein Lipase - metabolism ; Muscle, Skeletal - cytology ; Muscle, Skeletal - metabolism ; PPAR gamma - genetics ; PPAR gamma - metabolism ; Primary Cell Culture ; Promoter Regions, Genetic ; Protein Binding ; Protein Isoforms - genetics ; Protein Isoforms - metabolism ; RNA, Small Interfering - genetics ; RNA, Small Interfering - metabolism ; Signal Transduction ; siRNA interference ; Skin - cytology ; Skin - metabolism ; subcutaneous adipocytes</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2020-08, Vol.84 (8), p.1594-1602</ispartof><rights>2020 Japan Society for Bioscience, Biotechnology, and Agrochemistry 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-9dc9c2ae7a180dfda464957cdb583ecd4058c5c2079e3ba1fbcb3cc258827d033</citedby><cites>FETCH-LOGICAL-c431t-9dc9c2ae7a180dfda464957cdb583ecd4058c5c2079e3ba1fbcb3cc258827d033</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32434447$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, Qing</creatorcontrib><creatorcontrib>Lin, Yaqiu</creatorcontrib><creatorcontrib>Wang, Yong</creatorcontrib><creatorcontrib>Bai, Wenlin</creatorcontrib><creatorcontrib>Zhu, Jiangjiang</creatorcontrib><title>Knockdown of KLF9 promotes the differentiation of both intramuscular and subcutaneous preadipocytes in goat</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>KLF9 is reported to promote adipocyte differentiation in 3T3-L1 cells and pigs. However, the roles of KLF9 in adipocytes differentiation of goat remain unknown. In this study, the expression profiles of KLF9 were different between subcutaneous and intramuscular preadipocytes of goat during differentiation process. After silencing KLF9 gene, the lipid droplets were increased in both two types of adipocytes. In subcutaneous preadipocyte with silencing KLF9, the expressions of C/EBPβ, PPARγ, LPL, KLF1-2, KLF5, and KLF17 genes were up-regulated, while KLF12, KLF4, and KLF13 genes were down-regulated in expression level. In intramuscular preadipocyte, aP2, C/EBPα, KLF2-3, KLF5, and KLF7 gene were up-regulated, and Pref-1 gene was down-regulated. In addition, the binding sites of KLF9 existed in the promoters of aP2, C/EBPα, C/EBPβ, LPL and Pref-1. Taken together, KLF9 play a negative role in the differentiation of both intramuscular and subcutaneous preadipocytes in goats, but the functional mechanism may be different.
Knockdown KLF9 promotes differentiation of intramuscular and subcutaneous preadipocyte, however, the mechanism of action might be different.</description><subject>Adipocytes - cytology</subject><subject>Adipocytes - metabolism</subject><subject>Adipogenesis - genetics</subject><subject>Animals</subject><subject>Calcium-Binding Proteins - genetics</subject><subject>Calcium-Binding Proteins - metabolism</subject><subject>CCAAT-Enhancer-Binding Protein-beta - genetics</subject><subject>CCAAT-Enhancer-Binding Protein-beta - metabolism</subject><subject>Cell Differentiation - genetics</subject><subject>Fatty Acid-Binding Proteins - genetics</subject><subject>Fatty Acid-Binding Proteins - metabolism</subject><subject>Gene Expression Regulation</subject><subject>Goat</subject><subject>Goats</subject><subject>intramuscular adipocytes</subject><subject>KLF9</subject><subject>Kruppel-Like Transcription Factors - antagonists & inhibitors</subject><subject>Kruppel-Like Transcription Factors - genetics</subject><subject>Kruppel-Like Transcription Factors - metabolism</subject><subject>Lipoprotein Lipase - genetics</subject><subject>Lipoprotein Lipase - metabolism</subject><subject>Muscle, Skeletal - cytology</subject><subject>Muscle, Skeletal - metabolism</subject><subject>PPAR gamma - genetics</subject><subject>PPAR gamma - metabolism</subject><subject>Primary Cell Culture</subject><subject>Promoter Regions, Genetic</subject><subject>Protein Binding</subject><subject>Protein Isoforms - genetics</subject><subject>Protein Isoforms - metabolism</subject><subject>RNA, Small Interfering - genetics</subject><subject>RNA, Small Interfering - metabolism</subject><subject>Signal Transduction</subject><subject>siRNA interference</subject><subject>Skin - cytology</subject><subject>Skin - metabolism</subject><subject>subcutaneous adipocytes</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMFO3DAURS1EVaa0nwDykk2oHdtxsqNCBSpG6qZdW86zDYbEHmxHaP6-STOwrLqy9HTueX4XoTNKLilpyVfS0ablgl7WpJ5HspG8k0doQxmXVdNxeYw2C1Mt0An6lPMTIfNA0I_ohNWccc7lBj3fhwjPJr4GHB2-3950eJfiGIvNuDxabLxzNtlQvC4-_oX6WB6xDyXpccowDTphHQzOUw9T0cHGKc8Oq43fRdgvIh_wQ9TlM_rg9JDtl8N7in7ffP91fVdtf97-uP62rYAzWqrOQAe1tlLTlhhnNG94JySYXrTMguFEtCCgJrKzrNfU9dAzgFq0bS0NYewUXaze-ZKXyeaiRp_BDsP6OVXPBsYEbeiMihWFFHNO1qld8qNOe0WJWnpWbz2rpWd16HnOnR9WTP1ozXvqrdgZICsQp91_O6_WiA8uplG_xjQYVfR-iMklHcBnxf6t-APwNZxw</recordid><startdate>20200802</startdate><enddate>20200802</enddate><creator>Xu, Qing</creator><creator>Lin, Yaqiu</creator><creator>Wang, Yong</creator><creator>Bai, Wenlin</creator><creator>Zhu, Jiangjiang</creator><general>Taylor & Francis</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20200802</creationdate><title>Knockdown of KLF9 promotes the differentiation of both intramuscular and subcutaneous preadipocytes in goat</title><author>Xu, Qing ; Lin, Yaqiu ; Wang, Yong ; Bai, Wenlin ; Zhu, Jiangjiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-9dc9c2ae7a180dfda464957cdb583ecd4058c5c2079e3ba1fbcb3cc258827d033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adipocytes - cytology</topic><topic>Adipocytes - metabolism</topic><topic>Adipogenesis - genetics</topic><topic>Animals</topic><topic>Calcium-Binding Proteins - genetics</topic><topic>Calcium-Binding Proteins - metabolism</topic><topic>CCAAT-Enhancer-Binding Protein-beta - genetics</topic><topic>CCAAT-Enhancer-Binding Protein-beta - metabolism</topic><topic>Cell Differentiation - genetics</topic><topic>Fatty Acid-Binding Proteins - genetics</topic><topic>Fatty Acid-Binding Proteins - metabolism</topic><topic>Gene Expression Regulation</topic><topic>Goat</topic><topic>Goats</topic><topic>intramuscular adipocytes</topic><topic>KLF9</topic><topic>Kruppel-Like Transcription Factors - antagonists & inhibitors</topic><topic>Kruppel-Like Transcription Factors - genetics</topic><topic>Kruppel-Like Transcription Factors - metabolism</topic><topic>Lipoprotein Lipase - genetics</topic><topic>Lipoprotein Lipase - metabolism</topic><topic>Muscle, Skeletal - cytology</topic><topic>Muscle, Skeletal - metabolism</topic><topic>PPAR gamma - genetics</topic><topic>PPAR gamma - metabolism</topic><topic>Primary Cell Culture</topic><topic>Promoter Regions, Genetic</topic><topic>Protein Binding</topic><topic>Protein Isoforms - genetics</topic><topic>Protein Isoforms - metabolism</topic><topic>RNA, Small Interfering - genetics</topic><topic>RNA, Small Interfering - metabolism</topic><topic>Signal Transduction</topic><topic>siRNA interference</topic><topic>Skin - cytology</topic><topic>Skin - metabolism</topic><topic>subcutaneous adipocytes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Qing</creatorcontrib><creatorcontrib>Lin, Yaqiu</creatorcontrib><creatorcontrib>Wang, Yong</creatorcontrib><creatorcontrib>Bai, Wenlin</creatorcontrib><creatorcontrib>Zhu, Jiangjiang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Qing</au><au>Lin, Yaqiu</au><au>Wang, Yong</au><au>Bai, Wenlin</au><au>Zhu, Jiangjiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Knockdown of KLF9 promotes the differentiation of both intramuscular and subcutaneous preadipocytes in goat</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2020-08-02</date><risdate>2020</risdate><volume>84</volume><issue>8</issue><spage>1594</spage><epage>1602</epage><pages>1594-1602</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>KLF9 is reported to promote adipocyte differentiation in 3T3-L1 cells and pigs. However, the roles of KLF9 in adipocytes differentiation of goat remain unknown. In this study, the expression profiles of KLF9 were different between subcutaneous and intramuscular preadipocytes of goat during differentiation process. After silencing KLF9 gene, the lipid droplets were increased in both two types of adipocytes. In subcutaneous preadipocyte with silencing KLF9, the expressions of C/EBPβ, PPARγ, LPL, KLF1-2, KLF5, and KLF17 genes were up-regulated, while KLF12, KLF4, and KLF13 genes were down-regulated in expression level. In intramuscular preadipocyte, aP2, C/EBPα, KLF2-3, KLF5, and KLF7 gene were up-regulated, and Pref-1 gene was down-regulated. In addition, the binding sites of KLF9 existed in the promoters of aP2, C/EBPα, C/EBPβ, LPL and Pref-1. Taken together, KLF9 play a negative role in the differentiation of both intramuscular and subcutaneous preadipocytes in goats, but the functional mechanism may be different.
Knockdown KLF9 promotes differentiation of intramuscular and subcutaneous preadipocyte, however, the mechanism of action might be different.</abstract><cop>England</cop><pub>Taylor & Francis</pub><pmid>32434447</pmid><doi>10.1080/09168451.2020.1767497</doi><tpages>9</tpages></addata></record> |
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| source | MEDLINE; Freely Accessible Japanese Titles; Oxford Academic Journals (OUP); EZB Electronic Journals Library |
| subjects | Adipocytes - cytology Adipocytes - metabolism Adipogenesis - genetics Animals Calcium-Binding Proteins - genetics Calcium-Binding Proteins - metabolism CCAAT-Enhancer-Binding Protein-beta - genetics CCAAT-Enhancer-Binding Protein-beta - metabolism Cell Differentiation - genetics Fatty Acid-Binding Proteins - genetics Fatty Acid-Binding Proteins - metabolism Gene Expression Regulation Goat Goats intramuscular adipocytes KLF9 Kruppel-Like Transcription Factors - antagonists & inhibitors Kruppel-Like Transcription Factors - genetics Kruppel-Like Transcription Factors - metabolism Lipoprotein Lipase - genetics Lipoprotein Lipase - metabolism Muscle, Skeletal - cytology Muscle, Skeletal - metabolism PPAR gamma - genetics PPAR gamma - metabolism Primary Cell Culture Promoter Regions, Genetic Protein Binding Protein Isoforms - genetics Protein Isoforms - metabolism RNA, Small Interfering - genetics RNA, Small Interfering - metabolism Signal Transduction siRNA interference Skin - cytology Skin - metabolism subcutaneous adipocytes |
| title | Knockdown of KLF9 promotes the differentiation of both intramuscular and subcutaneous preadipocytes in goat |
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