MicroRNA-5195-3p plays a suppressive role in cell proliferation, migration and invasion by targeting MYO6 in human non-small cell lung cancer
MiRNA-5195-3p (miR-5195-3p), a recently discovered and poorly studied miRNA, has been reported to suppress bladder cancer cell behavior. However, its regulatory role in non-small cell lung cancer (NSCLC) remains unclear. Here, the expression of miR-5195-3p was found to be reduced in NSCLC tissues an...
Gespeichert in:
| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2019-02, Vol.83 (2), p.212-220 |
|---|---|
| 1. Verfasser: | |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 220 |
|---|---|
| container_issue | 2 |
| container_start_page | 212 |
| container_title | Bioscience, biotechnology, and biochemistry |
| container_volume | 83 |
| creator | Yang, Quanfu |
| description | MiRNA-5195-3p (miR-5195-3p), a recently discovered and poorly studied miRNA, has been reported to suppress bladder cancer cell behavior. However, its regulatory role in non-small cell lung cancer (NSCLC) remains unclear. Here, the expression of miR-5195-3p was found to be reduced in NSCLC tissues and cells. The in vitro experiments showed that miR-5195-3p upregulation repressed cell proliferation, migration and invasion by CCK-8 and transwell assays. In addition, MYO6 was predicted and confirmed as a potential target of miR-5195-3p by Bioinformatics analysis, Luciferase reporter assay and western blot analysis. There was significantly negative correlation between miR-5195-3p and MYO6 in NSCLC tissues. Furthermore, MYO6 knockdown exhibited similar effects to those of miR-5195-3p overexpression in NSCLC cells, and restored MYO6 expression reversed the inhibitory effects of miR-5195-3p. Therefore, these results demonstrate that miR-5195-3p functions as a tumor suppressor by directly modulating MYO6 expression in NSCLC cells, and may be an innovative candidate target for NSCLC therapy.
MicroRNA-5195-3p plays a suppressive role in cell proliferation, migration and invasion by targeting MYO6 in human non-small cell lung cancer. |
| doi_str_mv | 10.1080/09168451.2018.1540288 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1080_09168451_2018_1540288</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1080/09168451.2018.1540288</oup_id><sourcerecordid>2129531546</sourcerecordid><originalsourceid>FETCH-LOGICAL-c478t-575d827f76d85c585b00cffc229ccd17402fd386c35a0ff988cea5fbadf8496b3</originalsourceid><addsrcrecordid>eNqNkVFv1SAcxcmi2a7TjzDDow_rFUop9G3LsqnJ5hKjDz6RfyncYVrooJ25H8LvLLV3Phqf4E9-5wDnIHRGyZYSSd6Thtay4nRbEiq3lFeklPIIbSirRFE3lXiBNgtTLNAJepXSD0LyAafH6IQRJgUTfIN-3Tkdw5fPlwWnDS_YiMce9gkDTvM4RpOSezI4ht5g57E2fY_HPDlrIkwu-HM8uN26xeC7DD1BWoZ2jyeIOzM5v8N33-_rRf8wD-CxD75IA2SrP379nAkNXpv4Gr200Cfz5rCeom8311-vPha39x8-XV3eFroSciq44J0shRV1J7nmkreEaGt1WTZad1TkLGzHZK0ZB2JtI6U2wG0LnZVVU7fsFL1bffNfHmeTJjW4tDwGvAlzUiUtG85yqHVG-YrmmFKKxqoxugHiXlGilibUcxNqaUIdmsi6t4cr5nYw3V_Vc_QZICsQ5vG_PS9WifM2xAF-hth3aoJ9H6KNOUGXFPu3xW9exKae</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2129531546</pqid></control><display><type>article</type><title>MicroRNA-5195-3p plays a suppressive role in cell proliferation, migration and invasion by targeting MYO6 in human non-small cell lung cancer</title><source>MEDLINE</source><source>Freely Accessible Japanese Titles (ERDB Project)</source><source>EZB Electronic Journals Library</source><source>Oxford Journals</source><creator>Yang, Quanfu</creator><creatorcontrib>Yang, Quanfu</creatorcontrib><description>MiRNA-5195-3p (miR-5195-3p), a recently discovered and poorly studied miRNA, has been reported to suppress bladder cancer cell behavior. However, its regulatory role in non-small cell lung cancer (NSCLC) remains unclear. Here, the expression of miR-5195-3p was found to be reduced in NSCLC tissues and cells. The in vitro experiments showed that miR-5195-3p upregulation repressed cell proliferation, migration and invasion by CCK-8 and transwell assays. In addition, MYO6 was predicted and confirmed as a potential target of miR-5195-3p by Bioinformatics analysis, Luciferase reporter assay and western blot analysis. There was significantly negative correlation between miR-5195-3p and MYO6 in NSCLC tissues. Furthermore, MYO6 knockdown exhibited similar effects to those of miR-5195-3p overexpression in NSCLC cells, and restored MYO6 expression reversed the inhibitory effects of miR-5195-3p. Therefore, these results demonstrate that miR-5195-3p functions as a tumor suppressor by directly modulating MYO6 expression in NSCLC cells, and may be an innovative candidate target for NSCLC therapy.
MicroRNA-5195-3p plays a suppressive role in cell proliferation, migration and invasion by targeting MYO6 in human non-small cell lung cancer.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1080/09168451.2018.1540288</identifier><identifier>PMID: 30387375</identifier><language>eng</language><publisher>England: Taylor & Francis</publisher><subject>3' Untranslated Regions ; Carcinoma, Non-Small-Cell Lung - genetics ; Carcinoma, Non-Small-Cell Lung - pathology ; Cell Line, Tumor ; cell proliferation ; Cell Proliferation - genetics ; Down-Regulation ; Female ; Gene Knockdown Techniques ; Humans ; Lung Neoplasms - genetics ; Lung Neoplasms - pathology ; Male ; Middle Aged ; miR-5195-3p ; mobility ; MYO6 ; Myosin Heavy Chains - genetics ; Myosin Heavy Chains - metabolism ; Neoplasm Invasiveness - genetics ; Neoplasm Metastasis - genetics ; Non-small cell lung cancer ; Protein Binding ; Up-Regulation</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2019-02, Vol.83 (2), p.212-220</ispartof><rights>2018 Japan Society for Bioscience, Biotechnology, and Agrochemistry 2018</rights><rights>2019 Japan Society for Bioscience, Biotechnology, and Agrochemistry 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-575d827f76d85c585b00cffc229ccd17402fd386c35a0ff988cea5fbadf8496b3</citedby><cites>FETCH-LOGICAL-c478t-575d827f76d85c585b00cffc229ccd17402fd386c35a0ff988cea5fbadf8496b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30387375$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Quanfu</creatorcontrib><title>MicroRNA-5195-3p plays a suppressive role in cell proliferation, migration and invasion by targeting MYO6 in human non-small cell lung cancer</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>MiRNA-5195-3p (miR-5195-3p), a recently discovered and poorly studied miRNA, has been reported to suppress bladder cancer cell behavior. However, its regulatory role in non-small cell lung cancer (NSCLC) remains unclear. Here, the expression of miR-5195-3p was found to be reduced in NSCLC tissues and cells. The in vitro experiments showed that miR-5195-3p upregulation repressed cell proliferation, migration and invasion by CCK-8 and transwell assays. In addition, MYO6 was predicted and confirmed as a potential target of miR-5195-3p by Bioinformatics analysis, Luciferase reporter assay and western blot analysis. There was significantly negative correlation between miR-5195-3p and MYO6 in NSCLC tissues. Furthermore, MYO6 knockdown exhibited similar effects to those of miR-5195-3p overexpression in NSCLC cells, and restored MYO6 expression reversed the inhibitory effects of miR-5195-3p. Therefore, these results demonstrate that miR-5195-3p functions as a tumor suppressor by directly modulating MYO6 expression in NSCLC cells, and may be an innovative candidate target for NSCLC therapy.
MicroRNA-5195-3p plays a suppressive role in cell proliferation, migration and invasion by targeting MYO6 in human non-small cell lung cancer.</description><subject>3' Untranslated Regions</subject><subject>Carcinoma, Non-Small-Cell Lung - genetics</subject><subject>Carcinoma, Non-Small-Cell Lung - pathology</subject><subject>Cell Line, Tumor</subject><subject>cell proliferation</subject><subject>Cell Proliferation - genetics</subject><subject>Down-Regulation</subject><subject>Female</subject><subject>Gene Knockdown Techniques</subject><subject>Humans</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - pathology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>miR-5195-3p</subject><subject>mobility</subject><subject>MYO6</subject><subject>Myosin Heavy Chains - genetics</subject><subject>Myosin Heavy Chains - metabolism</subject><subject>Neoplasm Invasiveness - genetics</subject><subject>Neoplasm Metastasis - genetics</subject><subject>Non-small cell lung cancer</subject><subject>Protein Binding</subject><subject>Up-Regulation</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkVFv1SAcxcmi2a7TjzDDow_rFUop9G3LsqnJ5hKjDz6RfyncYVrooJ25H8LvLLV3Phqf4E9-5wDnIHRGyZYSSd6Thtay4nRbEiq3lFeklPIIbSirRFE3lXiBNgtTLNAJepXSD0LyAafH6IQRJgUTfIN-3Tkdw5fPlwWnDS_YiMce9gkDTvM4RpOSezI4ht5g57E2fY_HPDlrIkwu-HM8uN26xeC7DD1BWoZ2jyeIOzM5v8N33-_rRf8wD-CxD75IA2SrP379nAkNXpv4Gr200Cfz5rCeom8311-vPha39x8-XV3eFroSciq44J0shRV1J7nmkreEaGt1WTZad1TkLGzHZK0ZB2JtI6U2wG0LnZVVU7fsFL1bffNfHmeTJjW4tDwGvAlzUiUtG85yqHVG-YrmmFKKxqoxugHiXlGilibUcxNqaUIdmsi6t4cr5nYw3V_Vc_QZICsQ5vG_PS9WifM2xAF-hth3aoJ9H6KNOUGXFPu3xW9exKae</recordid><startdate>201902</startdate><enddate>201902</enddate><creator>Yang, Quanfu</creator><general>Taylor & Francis</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201902</creationdate><title>MicroRNA-5195-3p plays a suppressive role in cell proliferation, migration and invasion by targeting MYO6 in human non-small cell lung cancer</title><author>Yang, Quanfu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-575d827f76d85c585b00cffc229ccd17402fd386c35a0ff988cea5fbadf8496b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>3' Untranslated Regions</topic><topic>Carcinoma, Non-Small-Cell Lung - genetics</topic><topic>Carcinoma, Non-Small-Cell Lung - pathology</topic><topic>Cell Line, Tumor</topic><topic>cell proliferation</topic><topic>Cell Proliferation - genetics</topic><topic>Down-Regulation</topic><topic>Female</topic><topic>Gene Knockdown Techniques</topic><topic>Humans</topic><topic>Lung Neoplasms - genetics</topic><topic>Lung Neoplasms - pathology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>miR-5195-3p</topic><topic>mobility</topic><topic>MYO6</topic><topic>Myosin Heavy Chains - genetics</topic><topic>Myosin Heavy Chains - metabolism</topic><topic>Neoplasm Invasiveness - genetics</topic><topic>Neoplasm Metastasis - genetics</topic><topic>Non-small cell lung cancer</topic><topic>Protein Binding</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Quanfu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Quanfu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MicroRNA-5195-3p plays a suppressive role in cell proliferation, migration and invasion by targeting MYO6 in human non-small cell lung cancer</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2019-02</date><risdate>2019</risdate><volume>83</volume><issue>2</issue><spage>212</spage><epage>220</epage><pages>212-220</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>MiRNA-5195-3p (miR-5195-3p), a recently discovered and poorly studied miRNA, has been reported to suppress bladder cancer cell behavior. However, its regulatory role in non-small cell lung cancer (NSCLC) remains unclear. Here, the expression of miR-5195-3p was found to be reduced in NSCLC tissues and cells. The in vitro experiments showed that miR-5195-3p upregulation repressed cell proliferation, migration and invasion by CCK-8 and transwell assays. In addition, MYO6 was predicted and confirmed as a potential target of miR-5195-3p by Bioinformatics analysis, Luciferase reporter assay and western blot analysis. There was significantly negative correlation between miR-5195-3p and MYO6 in NSCLC tissues. Furthermore, MYO6 knockdown exhibited similar effects to those of miR-5195-3p overexpression in NSCLC cells, and restored MYO6 expression reversed the inhibitory effects of miR-5195-3p. Therefore, these results demonstrate that miR-5195-3p functions as a tumor suppressor by directly modulating MYO6 expression in NSCLC cells, and may be an innovative candidate target for NSCLC therapy.
MicroRNA-5195-3p plays a suppressive role in cell proliferation, migration and invasion by targeting MYO6 in human non-small cell lung cancer.</abstract><cop>England</cop><pub>Taylor & Francis</pub><pmid>30387375</pmid><doi>10.1080/09168451.2018.1540288</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0916-8451 |
| ispartof | Bioscience, biotechnology, and biochemistry, 2019-02, Vol.83 (2), p.212-220 |
| issn | 0916-8451 1347-6947 |
| language | eng |
| recordid | cdi_crossref_primary_10_1080_09168451_2018_1540288 |
| source | MEDLINE; Freely Accessible Japanese Titles (ERDB Project); EZB Electronic Journals Library; Oxford Journals |
| subjects | 3' Untranslated Regions Carcinoma, Non-Small-Cell Lung - genetics Carcinoma, Non-Small-Cell Lung - pathology Cell Line, Tumor cell proliferation Cell Proliferation - genetics Down-Regulation Female Gene Knockdown Techniques Humans Lung Neoplasms - genetics Lung Neoplasms - pathology Male Middle Aged miR-5195-3p mobility MYO6 Myosin Heavy Chains - genetics Myosin Heavy Chains - metabolism Neoplasm Invasiveness - genetics Neoplasm Metastasis - genetics Non-small cell lung cancer Protein Binding Up-Regulation |
| title | MicroRNA-5195-3p plays a suppressive role in cell proliferation, migration and invasion by targeting MYO6 in human non-small cell lung cancer |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T10%3A41%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=MicroRNA-5195-3p%20plays%20a%20suppressive%20role%20in%20cell%20proliferation,%20migration%20and%20invasion%20by%20targeting%20MYO6%20in%20human%20non-small%20cell%20lung%20cancer&rft.jtitle=Bioscience,%20biotechnology,%20and%20biochemistry&rft.au=Yang,%20Quanfu&rft.date=2019-02&rft.volume=83&rft.issue=2&rft.spage=212&rft.epage=220&rft.pages=212-220&rft.issn=0916-8451&rft.eissn=1347-6947&rft_id=info:doi/10.1080/09168451.2018.1540288&rft_dat=%3Cproquest_cross%3E2129531546%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2129531546&rft_id=info:pmid/30387375&rft_oup_id=10.1080/09168451.2018.1540288&rfr_iscdi=true |