A long natural-antisense RNA is accumulated in the conidia of Aspergillus oryzae
Analysis of expressed sequence tag libraries from various culture conditions revealed the existence of conidia-specific transcripts assembled to putative conidiation-specific reductase gene (csrA) in Aspergillus oryzae. However, the all transcripts were transcribed with opposite direction to the gen...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2016-02, Vol.80 (2), p.386-398 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | Tsujii, Masaru Okuda, Satoshi Ishi, Kazutomo Madokoro, Kana Takeuchi, Michio Yamagata, Youhei |
| description | Analysis of expressed sequence tag libraries from various culture conditions revealed the existence of conidia-specific transcripts assembled to putative conidiation-specific reductase gene (csrA) in Aspergillus oryzae. However, the all transcripts were transcribed with opposite direction to the gene csrA. The sequence analysis of the transcript revealed that the RNA overlapped mRNA of csrA with 3′-end, and did not code protein longer than 60 amino acid residues. We designated the transcript Conidia Specific Long Natural-antisense RNA (CSLNR). The real-time PCR analysis demonstrated that the CSLNR is conidia-specific transcript, which cannot be transcribed in the absence of brlA, and the amount of CSLNR was much more than that of the transcript from csrA in conidia. Furthermore, the csrA deletion, also lacking coding region of CSLNR in A. oryzae reduced the number of conidia. Overexpression of CsrA demonstrated the inhibition of growth and conidiation, while CSLNR did not affect conidiation.
The results of EST analysis and Real-time PCR showed CSLNR is abundant in the conidia of Aspergillus oryzae. Deletion of both csrA and CSLNR occurred reduction of conidiation. |
| doi_str_mv | 10.1080/09168451.2015.1101333 |
| format | Article |
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The results of EST analysis and Real-time PCR showed CSLNR is abundant in the conidia of Aspergillus oryzae. Deletion of both csrA and CSLNR occurred reduction of conidiation.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1080/09168451.2015.1101333</identifier><identifier>PMID: 26539735</identifier><language>eng</language><publisher>England: Taylor & Francis</publisher><subject>Aspergillus oryzae ; Aspergillus oryzae - genetics ; Aspergillus oryzae - metabolism ; Base Sequence ; CSLNR ; Exons ; Expressed Sequence Tags ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Gene Deletion ; Gene Expression Regulation, Fungal ; Introns ; long natural antisense RNA ; Molecular Sequence Data ; Real-Time Polymerase Chain Reaction ; RNA, Antisense - genetics ; RNA, Antisense - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Spores, Fungal - genetics ; Spores, Fungal - metabolism ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transcription, Genetic</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2016-02, Vol.80 (2), p.386-398</ispartof><rights>2015 Japan Society for Bioscience, Biotechnology, and Agrochemistry 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c437t-6ba3711df66aa3eeb7680c5ed9d3bca5f1f010f8bc231f7e3cb226aaea3a84893</citedby><cites>FETCH-LOGICAL-c437t-6ba3711df66aa3eeb7680c5ed9d3bca5f1f010f8bc231f7e3cb226aaea3a84893</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26539735$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsujii, Masaru</creatorcontrib><creatorcontrib>Okuda, Satoshi</creatorcontrib><creatorcontrib>Ishi, Kazutomo</creatorcontrib><creatorcontrib>Madokoro, Kana</creatorcontrib><creatorcontrib>Takeuchi, Michio</creatorcontrib><creatorcontrib>Yamagata, Youhei</creatorcontrib><title>A long natural-antisense RNA is accumulated in the conidia of Aspergillus oryzae</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>Analysis of expressed sequence tag libraries from various culture conditions revealed the existence of conidia-specific transcripts assembled to putative conidiation-specific reductase gene (csrA) in Aspergillus oryzae. However, the all transcripts were transcribed with opposite direction to the gene csrA. The sequence analysis of the transcript revealed that the RNA overlapped mRNA of csrA with 3′-end, and did not code protein longer than 60 amino acid residues. We designated the transcript Conidia Specific Long Natural-antisense RNA (CSLNR). The real-time PCR analysis demonstrated that the CSLNR is conidia-specific transcript, which cannot be transcribed in the absence of brlA, and the amount of CSLNR was much more than that of the transcript from csrA in conidia. Furthermore, the csrA deletion, also lacking coding region of CSLNR in A. oryzae reduced the number of conidia. Overexpression of CsrA demonstrated the inhibition of growth and conidiation, while CSLNR did not affect conidiation.
The results of EST analysis and Real-time PCR showed CSLNR is abundant in the conidia of Aspergillus oryzae. Deletion of both csrA and CSLNR occurred reduction of conidiation.</description><subject>Aspergillus oryzae</subject><subject>Aspergillus oryzae - genetics</subject><subject>Aspergillus oryzae - metabolism</subject><subject>Base Sequence</subject><subject>CSLNR</subject><subject>Exons</subject><subject>Expressed Sequence Tags</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Gene Deletion</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Introns</subject><subject>long natural antisense RNA</subject><subject>Molecular Sequence Data</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>RNA, Antisense - genetics</subject><subject>RNA, Antisense - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Spores, Fungal - genetics</subject><subject>Spores, Fungal - metabolism</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v1DAURS1ERYfCTwB5ySaDX15iJztGVfmQqhYhWFsvjl2MHHuwE1XDryejmbJk9aSnc--VDmNvQGxBdOK96EF2TQvbWkC7BRCAiM_YBrBRlewb9Zxtjkx1hC7Zy1J-CbE-WnjBLmvZYq-w3bCvOx5SfOCR5iVTqCjOvthYLP92t-O-cDJmmZZAsx25j3z-ablJ0Y-eeHJ8V_Y2P_gQlsJTPvwh-4pdOArFvj7fK_bj483368_V7f2nL9e728o0qOZKDoQKYHRSEqG1g5KdMK0d-xEHQ60DJ0C4bjA1glMWzVDXK2oJqWu6Hq_Yu1PvPqffiy2znnwxNgSKNi1Fg5KikxJRrWh7Qk1OpWTr9D77ifJBg9BHmfpJpj7K1GeZa-7teWIZJjv-Sz3ZW4EPJ8BHl_JEjymHUc90CCm7TNH4ovH_G38B7emDwg</recordid><startdate>20160201</startdate><enddate>20160201</enddate><creator>Tsujii, Masaru</creator><creator>Okuda, Satoshi</creator><creator>Ishi, Kazutomo</creator><creator>Madokoro, Kana</creator><creator>Takeuchi, Michio</creator><creator>Yamagata, Youhei</creator><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20160201</creationdate><title>A long natural-antisense RNA is accumulated in the conidia of Aspergillus oryzae</title><author>Tsujii, Masaru ; Okuda, Satoshi ; Ishi, Kazutomo ; Madokoro, Kana ; Takeuchi, Michio ; Yamagata, Youhei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437t-6ba3711df66aa3eeb7680c5ed9d3bca5f1f010f8bc231f7e3cb226aaea3a84893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Aspergillus oryzae</topic><topic>Aspergillus oryzae - genetics</topic><topic>Aspergillus oryzae - metabolism</topic><topic>Base Sequence</topic><topic>CSLNR</topic><topic>Exons</topic><topic>Expressed Sequence Tags</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>Gene Deletion</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Introns</topic><topic>long natural antisense RNA</topic><topic>Molecular Sequence Data</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>RNA, Antisense - genetics</topic><topic>RNA, Antisense - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Spores, Fungal - genetics</topic><topic>Spores, Fungal - metabolism</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsujii, Masaru</creatorcontrib><creatorcontrib>Okuda, Satoshi</creatorcontrib><creatorcontrib>Ishi, Kazutomo</creatorcontrib><creatorcontrib>Madokoro, Kana</creatorcontrib><creatorcontrib>Takeuchi, Michio</creatorcontrib><creatorcontrib>Yamagata, Youhei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsujii, Masaru</au><au>Okuda, Satoshi</au><au>Ishi, Kazutomo</au><au>Madokoro, Kana</au><au>Takeuchi, Michio</au><au>Yamagata, Youhei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A long natural-antisense RNA is accumulated in the conidia of Aspergillus oryzae</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2016-02-01</date><risdate>2016</risdate><volume>80</volume><issue>2</issue><spage>386</spage><epage>398</epage><pages>386-398</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>Analysis of expressed sequence tag libraries from various culture conditions revealed the existence of conidia-specific transcripts assembled to putative conidiation-specific reductase gene (csrA) in Aspergillus oryzae. However, the all transcripts were transcribed with opposite direction to the gene csrA. The sequence analysis of the transcript revealed that the RNA overlapped mRNA of csrA with 3′-end, and did not code protein longer than 60 amino acid residues. We designated the transcript Conidia Specific Long Natural-antisense RNA (CSLNR). The real-time PCR analysis demonstrated that the CSLNR is conidia-specific transcript, which cannot be transcribed in the absence of brlA, and the amount of CSLNR was much more than that of the transcript from csrA in conidia. Furthermore, the csrA deletion, also lacking coding region of CSLNR in A. oryzae reduced the number of conidia. Overexpression of CsrA demonstrated the inhibition of growth and conidiation, while CSLNR did not affect conidiation.
The results of EST analysis and Real-time PCR showed CSLNR is abundant in the conidia of Aspergillus oryzae. Deletion of both csrA and CSLNR occurred reduction of conidiation.</abstract><cop>England</cop><pub>Taylor & Francis</pub><pmid>26539735</pmid><doi>10.1080/09168451.2015.1101333</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Open Access Titles of Japan; EZB-FREE-00999 freely available EZB journals |
| subjects | Aspergillus oryzae Aspergillus oryzae - genetics Aspergillus oryzae - metabolism Base Sequence CSLNR Exons Expressed Sequence Tags Fungal Proteins - genetics Fungal Proteins - metabolism Gene Deletion Gene Expression Regulation, Fungal Introns long natural antisense RNA Molecular Sequence Data Real-Time Polymerase Chain Reaction RNA, Antisense - genetics RNA, Antisense - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Spores, Fungal - genetics Spores, Fungal - metabolism Transcription Factors - genetics Transcription Factors - metabolism Transcription, Genetic |
| title | A long natural-antisense RNA is accumulated in the conidia of Aspergillus oryzae |
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