Binding of the Glycosaminoglycan [3H] Heparin to Bovine Granulosa cells Varies with size and Estrogen Content of Ovarian Follicles
Bovine ovarian follicles were individually aspirated to obtain granulosa cells and follicular fluid, and estrogen concentrations in fluid were measured with a radioimmunoassay. Granulosa membranes were harvested by centrifugation and were pooled from follicles containing statistically high or low es...
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| Veröffentlicht in: | Endocrine research 1984, Vol.10 (1), p.63-72 |
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| container_title | Endocrine research |
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| creator | Ax, R. L. Bushmeyer, S. M. Boehm, S. K. Bellin, M. E. |
| description | Bovine ovarian follicles were individually aspirated to obtain granulosa cells and follicular fluid, and estrogen concentrations in fluid were measured with a radioimmunoassay. Granulosa membranes were harvested by centrifugation and were pooled from follicles containing statistically high or low estrogen. Granulosa cell concentrations were estimated by comparing their protein content to cell standards. Results showed that cell numbers increased with increasing follicle size, and follicles with high estrogen concentrations possessed more granulosa than follicles with low estrogen. Specific binding of [3H]heparin decreased with advancing follicle size and was higher to granulosa harvested from follicles containing low estrogen. |
| doi_str_mv | 10.1080/07435808409046766 |
| format | Article |
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Specific binding of [3H]heparin decreased with advancing follicle size and was higher to granulosa harvested from follicles containing low estrogen.</description><identifier>ISSN: 0743-5800</identifier><identifier>EISSN: 1532-4206</identifier><identifier>DOI: 10.1080/07435808409046766</identifier><identifier>PMID: 6540169</identifier><language>eng</language><publisher>England: Informa Healthcare USA, Inc</publisher><subject>Animals ; Cattle ; Estrogens - analysis ; Female ; Granulosa Cells - analysis ; Granulosa Cells - metabolism ; Heparin - metabolism ; Ovarian Follicle - analysis</subject><ispartof>Endocrine research, 1984, Vol.10 (1), p.63-72</ispartof><rights>1984 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1984</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-de02757bb9eaf52867f6fe4f89cf3e4c9066fa76cafb221037ab565088954eed3</citedby><cites>FETCH-LOGICAL-c316t-de02757bb9eaf52867f6fe4f89cf3e4c9066fa76cafb221037ab565088954eed3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/07435808409046766$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/07435808409046766$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>314,780,784,4024,27923,27924,27925,59647,59753,60436,60542,61221,61256,61402,61437</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6540169$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ax, R. L.</creatorcontrib><creatorcontrib>Bushmeyer, S. M.</creatorcontrib><creatorcontrib>Boehm, S. K.</creatorcontrib><creatorcontrib>Bellin, M. E.</creatorcontrib><title>Binding of the Glycosaminoglycan [3H] Heparin to Bovine Granulosa cells Varies with size and Estrogen Content of Ovarian Follicles</title><title>Endocrine research</title><addtitle>Endocr Res</addtitle><description>Bovine ovarian follicles were individually aspirated to obtain granulosa cells and follicular fluid, and estrogen concentrations in fluid were measured with a radioimmunoassay. Granulosa membranes were harvested by centrifugation and were pooled from follicles containing statistically high or low estrogen. Granulosa cell concentrations were estimated by comparing their protein content to cell standards. Results showed that cell numbers increased with increasing follicle size, and follicles with high estrogen concentrations possessed more granulosa than follicles with low estrogen. Specific binding of [3H]heparin decreased with advancing follicle size and was higher to granulosa harvested from follicles containing low estrogen.</description><subject>Animals</subject><subject>Cattle</subject><subject>Estrogens - analysis</subject><subject>Female</subject><subject>Granulosa Cells - analysis</subject><subject>Granulosa Cells - metabolism</subject><subject>Heparin - metabolism</subject><subject>Ovarian Follicle - analysis</subject><issn>0743-5800</issn><issn>1532-4206</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEGLFDEQhYMo67j6AzwIOXlrrXS602n04g67O8LCXtSLSJNOV2ayZJIxSe8yHv3lZphBEGFPVfC-96p4hLxm8I6BhPfQNbyVIBvooRGdEE_IgrW8rpoaxFOyOOhVAeA5eZHSHQDjAPyMnIm2ASb6Bfl9Yf1k_ZoGQ_MG6bXb65DU1vqwLqvy9Dtf_aAr3KloPc2BXoR76wsYlZ9dQalG5xL9VnRM9MHmDU32F1LlJ3qZcgxr9HQZfEafD1du7wtZcq-Cc1Y7TC_JM6NcwleneU6-Xl1-Wa6qm9vrz8tPN5XmTORqQqi7thvHHpVpayk6Iww2RvbacGx0D0IY1QmtzFjXDHinxla0IGXfNogTPydvj7m7GH7OmPKwtenwvPIY5jRIxoSQvSwgO4I6hpQimmEX7VbF_cBgOPQ-_Nd78bw5hc_jFqe_jlPRRf941K03IW7VQ4huGrLauxBNqVLbNPDH4j_8Y9-gcnmjVcThLszRl94eee4PJcOkaA</recordid><startdate>1984</startdate><enddate>1984</enddate><creator>Ax, R. 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E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-de02757bb9eaf52867f6fe4f89cf3e4c9066fa76cafb221037ab565088954eed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Animals</topic><topic>Cattle</topic><topic>Estrogens - analysis</topic><topic>Female</topic><topic>Granulosa Cells - analysis</topic><topic>Granulosa Cells - metabolism</topic><topic>Heparin - metabolism</topic><topic>Ovarian Follicle - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ax, R. L.</creatorcontrib><creatorcontrib>Bushmeyer, S. M.</creatorcontrib><creatorcontrib>Boehm, S. K.</creatorcontrib><creatorcontrib>Bellin, M. E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Endocrine research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ax, R. L.</au><au>Bushmeyer, S. M.</au><au>Boehm, S. K.</au><au>Bellin, M. E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Binding of the Glycosaminoglycan [3H] Heparin to Bovine Granulosa cells Varies with size and Estrogen Content of Ovarian Follicles</atitle><jtitle>Endocrine research</jtitle><addtitle>Endocr Res</addtitle><date>1984</date><risdate>1984</risdate><volume>10</volume><issue>1</issue><spage>63</spage><epage>72</epage><pages>63-72</pages><issn>0743-5800</issn><eissn>1532-4206</eissn><abstract>Bovine ovarian follicles were individually aspirated to obtain granulosa cells and follicular fluid, and estrogen concentrations in fluid were measured with a radioimmunoassay. Granulosa membranes were harvested by centrifugation and were pooled from follicles containing statistically high or low estrogen. Granulosa cell concentrations were estimated by comparing their protein content to cell standards. Results showed that cell numbers increased with increasing follicle size, and follicles with high estrogen concentrations possessed more granulosa than follicles with low estrogen. Specific binding of [3H]heparin decreased with advancing follicle size and was higher to granulosa harvested from follicles containing low estrogen.</abstract><cop>England</cop><pub>Informa Healthcare USA, Inc</pub><pmid>6540169</pmid><doi>10.1080/07435808409046766</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0743-5800 |
| ispartof | Endocrine research, 1984, Vol.10 (1), p.63-72 |
| issn | 0743-5800 1532-4206 |
| language | eng |
| recordid | cdi_crossref_primary_10_1080_07435808409046766 |
| source | MEDLINE; Taylor & Francis:Master (3349 titles); Taylor & Francis Medical Library - CRKN |
| subjects | Animals Cattle Estrogens - analysis Female Granulosa Cells - analysis Granulosa Cells - metabolism Heparin - metabolism Ovarian Follicle - analysis |
| title | Binding of the Glycosaminoglycan [3H] Heparin to Bovine Granulosa cells Varies with size and Estrogen Content of Ovarian Follicles |
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