Possibilities of Triplex-Formation by 21-Mer Oligodeoxyribonucleotides as Analogs of Tetrahymena Pre-rRNA Fragments

A potential DNA triple helix of 21-mer oligodeoxyribonucleotides was synthesized and characterized. The strands were chosen to study the interaction of internal guide and intervening sequences analogs as well as adjacent 3′and 5′exon parts around the splicing site of Tetrahymena pre-rRNA. Further in...

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Veröffentlicht in:Nucleosides & nucleotides 1998-09, Vol.17 (9-11), p.1905-1913
Hauptverfasser: Meister, W-V, Kargov, S. I., Birch-Hirschfeld, E., Bohley, C., Burckhardt, G., Bischoff, G., Bischoff, R., Gromann, U., Hoflfmann, S.
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container_end_page 1913
container_issue 9-11
container_start_page 1905
container_title Nucleosides & nucleotides
container_volume 17
creator Meister, W-V
Kargov, S. I.
Birch-Hirschfeld, E.
Bohley, C.
Burckhardt, G.
Bischoff, G.
Bischoff, R.
Gromann, U.
Hoflfmann, S.
description A potential DNA triple helix of 21-mer oligodeoxyribonucleotides was synthesized and characterized. The strands were chosen to study the interaction of internal guide and intervening sequences analogs as well as adjacent 3′and 5′exon parts around the splicing site of Tetrahymena pre-rRNA. Further in parallel works a series of different RNA and DNA strands was synthesized and combined yielding a suitable order of stability. Here we want to show an isolated examination of a DNA-strand triple helix with defined sequences containing a central mismatched base arrangement and T-A-T bases at the ends.
doi_str_mv 10.1080/07328319808004729
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title Possibilities of Triplex-Formation by 21-Mer Oligodeoxyribonucleotides as Analogs of Tetrahymena Pre-rRNA Fragments
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