Rapid and sensitive detection of Avibacterium paragallinarum in the presence of other bacteria using a 5' Taq nuclease assay: a new tool for diagnosing infectious coryza

A 5' Taq nuclease assay specific for Avibacterium paragallinarum was designed and optimized for use in diagnosing infectious coryza. The region chosen for assay design was one of known specificity for Av. paragallinarum. The assay detected Av. paragallinarum reference strains representing the t...

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Veröffentlicht in:	Avian pathology 2008-12, Vol.37 (6), p.599-604
Hauptverfasser:	Corney, B.G, Diallo, I.S, Wright, L, Hewitson, G, De Jong, A, Tolosa, X, Burrell, P, Duffy, P, Rodwell, B, Boyle, D.B, Blackall, P.J
Format:	Artikel
Sprache:	eng
Schlagworte:	accuracy Animal diseases Animals Avibacterium paragallinarum Bacteria Bacteriological Techniques - methods Birds cross reaction detection limit diagnostic techniques disease detection disease diagnosis Genes, Bacterial Gram-Negative Bacteria - classification Gram-Negative Bacteria - isolation & purification Gram-Negative Bacterial Infections - diagnosis Gram-Negative Bacterial Infections - microbiology Gram-Negative Bacterial Infections - veterinary infectious coryza nucleases nucleotide sequences Optimization Ornithology Pasteurella pathogen identification Poultry Diseases - diagnosis Poultry Diseases - microbiology quantitative analysis Reproducibility of Results Sensitivity and Specificity Taq Polymerase - metabolism
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