The Use of Glucosamine and Actinomycin D in Radioactive Labeling of RNA in Cells in Culture

Pretreatment of confluent cultures of mouse L cells or of well-differentiated nervous system cells in primary cultures with 20-120 mM glucosamine resulted in a stimulation of the uptake of tritiated uridine, but not of adenosine. A marked stimulation of the incorporation of radioactive uridine into...

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Veröffentlicht in:	Preparative biochemistry 1977-01, Vol.7 (6), p.427-439
Hauptverfasser:	Grouse, Lawrence D., Schrier, Bruce K.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cells, Cultured Dactinomycin - pharmacology Glucosamine - pharmacology Kinetics L Cells (Cell Line) - drug effects L Cells (Cell Line) - metabolism Mice RNA - biosynthesis Spinal Cord - metabolism Subcellular Fractions - metabolism Transcription, Genetic - drug effects
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