Spindle pole body duplication defective yeast cells are more prone to membrane damage

Correct separation of chromosomes during mitosis is essential for preventing genetic instability and aneuploidy. Such separation is dependent on correct duplication of the nuclear-associated microtubular organizing center, i.e., spindle pole body (SPB), in fungi. MonoPolar Spindle 2 (MPS2) is an ess...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Mycologia 2019-11, Vol.111 (6), p.895-903
Hauptverfasser: Konuk, Hatice Büşra, Ergüden, Bengü
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 903
container_issue 6
container_start_page 895
container_title Mycologia
container_volume 111
creator Konuk, Hatice Büşra
Ergüden, Bengü
description Correct separation of chromosomes during mitosis is essential for preventing genetic instability and aneuploidy. Such separation is dependent on correct duplication of the nuclear-associated microtubular organizing center, i.e., spindle pole body (SPB), in fungi. MonoPolar Spindle 2 (MPS2) is an essential gene, encoding a membrane protein required for the insertion of SPB into the nuclear envelope. We recently reported that the SESA complex, which is composed of S my2, E ap1, S cp160, and A sc1, suppresses the essential role of MPS2 (Sezen et al. 2009, Genes & Development 23:1559-1570), i.e., in SESA-active cells Mps2 becomes nonessential. We also proposed that the SESA network facilitates this insertion by altering the membrane lipid composition (Sezen 2015, FEMS Yeast Research 15:fov089). In addition, we are interested in the antifungal properties of essential oils and previously reported that membrane integrity of yeast cells is impaired upon exposure to turpentine, thyme, oregano, and orange peel essential oils (Konuk and Ergüden 2017, BioCell 41:13-18). Due to our continuing interest in the SESA system and the mechanisms by which essential oils affect yeast cells, we aimed to investigate the effects of essential oils on yeast cell membranes. Herein, we show that mps2∆ 2µm-SMY2 and mps2∆ pom34∆ cells, in which the SESA complex is active and SPB duplication is defective, are more prone to membrane damage upon treatment with essential oils.
doi_str_mv 10.1080/00275514.2019.1659091
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1080_00275514_2019_1659091</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2303201571</sourcerecordid><originalsourceid>FETCH-LOGICAL-c413t-142f5e17415963d59580d8782f764acff0e2d991290feab8b517c738f2c7eca73</originalsourceid><addsrcrecordid>eNp9kD1PwzAQhi0EoqXwE0AeWVJ8TlwnGwjxJVVigM6WY59RUFIHOwX13-OoLSPL2Zafu3v1EHIJbA6sZDeMcSkEFHPOoJrDQlSsgiMyBSFkxkW-OCbTkclGaELOYvxMz_TLTskkB1EtoIQpWb31zdq2SHufSu3tltpN3zZGD41fU4sOzdB8I92ijgM12LaR6oC086n0wa-RDp522NVBp7vVnf7Ac3LidBvxYn_OyOrx4f3-OVu-Pr3c3y0zU0A-ZFBwJxBkMcbJrahEyWwpS-7kotDGOYbcVhXwijnUdVkLkEbmpeNGotEyn5Hr3dwU5GuDcVBdE8eMKYrfRMVzlic_QkJCxQ41wccY0Kk-NJ0OWwVMjUbVwagajaq90dR3tV-xqTu0f10HhQm43QHN2vnQ6R8fWqsGvW19cMmJaWKC_93xC2ADhL8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2303201571</pqid></control><display><type>article</type><title>Spindle pole body duplication defective yeast cells are more prone to membrane damage</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Konuk, Hatice Büşra ; Ergüden, Bengü</creator><creatorcontrib>Konuk, Hatice Büşra ; Ergüden, Bengü</creatorcontrib><description>Correct separation of chromosomes during mitosis is essential for preventing genetic instability and aneuploidy. Such separation is dependent on correct duplication of the nuclear-associated microtubular organizing center, i.e., spindle pole body (SPB), in fungi. MonoPolar Spindle 2 (MPS2) is an essential gene, encoding a membrane protein required for the insertion of SPB into the nuclear envelope. We recently reported that the SESA complex, which is composed of S my2, E ap1, S cp160, and A sc1, suppresses the essential role of MPS2 (Sezen et al. 2009, Genes &amp; Development 23:1559-1570), i.e., in SESA-active cells Mps2 becomes nonessential. We also proposed that the SESA network facilitates this insertion by altering the membrane lipid composition (Sezen 2015, FEMS Yeast Research 15:fov089). In addition, we are interested in the antifungal properties of essential oils and previously reported that membrane integrity of yeast cells is impaired upon exposure to turpentine, thyme, oregano, and orange peel essential oils (Konuk and Ergüden 2017, BioCell 41:13-18). Due to our continuing interest in the SESA system and the mechanisms by which essential oils affect yeast cells, we aimed to investigate the effects of essential oils on yeast cell membranes. Herein, we show that mps2∆ 2µm-SMY2 and mps2∆ pom34∆ cells, in which the SESA complex is active and SPB duplication is defective, are more prone to membrane damage upon treatment with essential oils.</description><identifier>ISSN: 0027-5514</identifier><identifier>EISSN: 1557-2536</identifier><identifier>DOI: 10.1080/00275514.2019.1659091</identifier><identifier>PMID: 31596181</identifier><language>eng</language><publisher>England: Taylor &amp; Francis</publisher><subject>Antifungal Agents - isolation &amp; purification ; Antifungal Agents - pharmacology ; Cell Membrane - drug effects ; Essential oil ; Gene Deletion ; membrane damage ; Mps2 ; Oils, Volatile - isolation &amp; purification ; Oils, Volatile - pharmacology ; Plants - chemistry ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - drug effects ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - growth &amp; development ; Saccharomyces cerevisiae Proteins - genetics ; Saccharomyces cerevisiae Proteins - metabolism ; Spindle Pole Bodies - genetics ; Spindle Pole Bodies - metabolism ; spindle pole body duplication</subject><ispartof>Mycologia, 2019-11, Vol.111 (6), p.895-903</ispartof><rights>2019 The Mycological Society of America 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-142f5e17415963d59580d8782f764acff0e2d991290feab8b517c738f2c7eca73</citedby><cites>FETCH-LOGICAL-c413t-142f5e17415963d59580d8782f764acff0e2d991290feab8b517c738f2c7eca73</cites><orcidid>0000-0002-8621-3474</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31596181$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Konuk, Hatice Büşra</creatorcontrib><creatorcontrib>Ergüden, Bengü</creatorcontrib><title>Spindle pole body duplication defective yeast cells are more prone to membrane damage</title><title>Mycologia</title><addtitle>Mycologia</addtitle><description>Correct separation of chromosomes during mitosis is essential for preventing genetic instability and aneuploidy. Such separation is dependent on correct duplication of the nuclear-associated microtubular organizing center, i.e., spindle pole body (SPB), in fungi. MonoPolar Spindle 2 (MPS2) is an essential gene, encoding a membrane protein required for the insertion of SPB into the nuclear envelope. We recently reported that the SESA complex, which is composed of S my2, E ap1, S cp160, and A sc1, suppresses the essential role of MPS2 (Sezen et al. 2009, Genes &amp; Development 23:1559-1570), i.e., in SESA-active cells Mps2 becomes nonessential. We also proposed that the SESA network facilitates this insertion by altering the membrane lipid composition (Sezen 2015, FEMS Yeast Research 15:fov089). In addition, we are interested in the antifungal properties of essential oils and previously reported that membrane integrity of yeast cells is impaired upon exposure to turpentine, thyme, oregano, and orange peel essential oils (Konuk and Ergüden 2017, BioCell 41:13-18). Due to our continuing interest in the SESA system and the mechanisms by which essential oils affect yeast cells, we aimed to investigate the effects of essential oils on yeast cell membranes. Herein, we show that mps2∆ 2µm-SMY2 and mps2∆ pom34∆ cells, in which the SESA complex is active and SPB duplication is defective, are more prone to membrane damage upon treatment with essential oils.</description><subject>Antifungal Agents - isolation &amp; purification</subject><subject>Antifungal Agents - pharmacology</subject><subject>Cell Membrane - drug effects</subject><subject>Essential oil</subject><subject>Gene Deletion</subject><subject>membrane damage</subject><subject>Mps2</subject><subject>Oils, Volatile - isolation &amp; purification</subject><subject>Oils, Volatile - pharmacology</subject><subject>Plants - chemistry</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - drug effects</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - growth &amp; development</subject><subject>Saccharomyces cerevisiae Proteins - genetics</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><subject>Spindle Pole Bodies - genetics</subject><subject>Spindle Pole Bodies - metabolism</subject><subject>spindle pole body duplication</subject><issn>0027-5514</issn><issn>1557-2536</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD1PwzAQhi0EoqXwE0AeWVJ8TlwnGwjxJVVigM6WY59RUFIHOwX13-OoLSPL2Zafu3v1EHIJbA6sZDeMcSkEFHPOoJrDQlSsgiMyBSFkxkW-OCbTkclGaELOYvxMz_TLTskkB1EtoIQpWb31zdq2SHufSu3tltpN3zZGD41fU4sOzdB8I92ijgM12LaR6oC086n0wa-RDp522NVBp7vVnf7Ac3LidBvxYn_OyOrx4f3-OVu-Pr3c3y0zU0A-ZFBwJxBkMcbJrahEyWwpS-7kotDGOYbcVhXwijnUdVkLkEbmpeNGotEyn5Hr3dwU5GuDcVBdE8eMKYrfRMVzlic_QkJCxQ41wccY0Kk-NJ0OWwVMjUbVwagajaq90dR3tV-xqTu0f10HhQm43QHN2vnQ6R8fWqsGvW19cMmJaWKC_93xC2ADhL8</recordid><startdate>20191102</startdate><enddate>20191102</enddate><creator>Konuk, Hatice Büşra</creator><creator>Ergüden, Bengü</creator><general>Taylor &amp; Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8621-3474</orcidid></search><sort><creationdate>20191102</creationdate><title>Spindle pole body duplication defective yeast cells are more prone to membrane damage</title><author>Konuk, Hatice Büşra ; Ergüden, Bengü</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-142f5e17415963d59580d8782f764acff0e2d991290feab8b517c738f2c7eca73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Antifungal Agents - isolation &amp; purification</topic><topic>Antifungal Agents - pharmacology</topic><topic>Cell Membrane - drug effects</topic><topic>Essential oil</topic><topic>Gene Deletion</topic><topic>membrane damage</topic><topic>Mps2</topic><topic>Oils, Volatile - isolation &amp; purification</topic><topic>Oils, Volatile - pharmacology</topic><topic>Plants - chemistry</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - drug effects</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - growth &amp; development</topic><topic>Saccharomyces cerevisiae Proteins - genetics</topic><topic>Saccharomyces cerevisiae Proteins - metabolism</topic><topic>Spindle Pole Bodies - genetics</topic><topic>Spindle Pole Bodies - metabolism</topic><topic>spindle pole body duplication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Konuk, Hatice Büşra</creatorcontrib><creatorcontrib>Ergüden, Bengü</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Mycologia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Konuk, Hatice Büşra</au><au>Ergüden, Bengü</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Spindle pole body duplication defective yeast cells are more prone to membrane damage</atitle><jtitle>Mycologia</jtitle><addtitle>Mycologia</addtitle><date>2019-11-02</date><risdate>2019</risdate><volume>111</volume><issue>6</issue><spage>895</spage><epage>903</epage><pages>895-903</pages><issn>0027-5514</issn><eissn>1557-2536</eissn><abstract>Correct separation of chromosomes during mitosis is essential for preventing genetic instability and aneuploidy. Such separation is dependent on correct duplication of the nuclear-associated microtubular organizing center, i.e., spindle pole body (SPB), in fungi. MonoPolar Spindle 2 (MPS2) is an essential gene, encoding a membrane protein required for the insertion of SPB into the nuclear envelope. We recently reported that the SESA complex, which is composed of S my2, E ap1, S cp160, and A sc1, suppresses the essential role of MPS2 (Sezen et al. 2009, Genes &amp; Development 23:1559-1570), i.e., in SESA-active cells Mps2 becomes nonessential. We also proposed that the SESA network facilitates this insertion by altering the membrane lipid composition (Sezen 2015, FEMS Yeast Research 15:fov089). In addition, we are interested in the antifungal properties of essential oils and previously reported that membrane integrity of yeast cells is impaired upon exposure to turpentine, thyme, oregano, and orange peel essential oils (Konuk and Ergüden 2017, BioCell 41:13-18). Due to our continuing interest in the SESA system and the mechanisms by which essential oils affect yeast cells, we aimed to investigate the effects of essential oils on yeast cell membranes. Herein, we show that mps2∆ 2µm-SMY2 and mps2∆ pom34∆ cells, in which the SESA complex is active and SPB duplication is defective, are more prone to membrane damage upon treatment with essential oils.</abstract><cop>England</cop><pub>Taylor &amp; Francis</pub><pmid>31596181</pmid><doi>10.1080/00275514.2019.1659091</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-8621-3474</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0027-5514
ispartof Mycologia, 2019-11, Vol.111 (6), p.895-903
issn 0027-5514
1557-2536
language eng
recordid cdi_crossref_primary_10_1080_00275514_2019_1659091
source MEDLINE; Alma/SFX Local Collection
subjects Antifungal Agents - isolation & purification
Antifungal Agents - pharmacology
Cell Membrane - drug effects
Essential oil
Gene Deletion
membrane damage
Mps2
Oils, Volatile - isolation & purification
Oils, Volatile - pharmacology
Plants - chemistry
Saccharomyces cerevisiae
Saccharomyces cerevisiae - drug effects
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Spindle Pole Bodies - genetics
Spindle Pole Bodies - metabolism
spindle pole body duplication
title Spindle pole body duplication defective yeast cells are more prone to membrane damage
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T09%3A25%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Spindle%20pole%20body%20duplication%20defective%20yeast%20cells%20are%20more%20prone%20to%20membrane%20damage&rft.jtitle=Mycologia&rft.au=Konuk,%20Hatice%20B%C3%BC%C5%9Fra&rft.date=2019-11-02&rft.volume=111&rft.issue=6&rft.spage=895&rft.epage=903&rft.pages=895-903&rft.issn=0027-5514&rft.eissn=1557-2536&rft_id=info:doi/10.1080/00275514.2019.1659091&rft_dat=%3Cproquest_cross%3E2303201571%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2303201571&rft_id=info:pmid/31596181&rfr_iscdi=true