Molecular Characterization of Ochratoxin A Producing Strains of the Genus Penicillium
Sixty-six strains classified as P. verrucosum based on morphological criteria were characterized by molecular methods like RAPD, AFLP and ITS sequencing. Two groups could be identified by RAPD and AFLP analyses. The two RAPD as well as the two AFLP groups were completely coincidental. Strains in the...
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| Veröffentlicht in: | Systematic and applied microbiology 2002-04, Vol.25 (1), p.74-83 |
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| creator | Castella, Gemma Ostenfeld Larsen, Thomas Cabanes, Javier Schmidt, Holger Alboresi, Alessandro Niessen, Ludwig Färber, Paul Geisen, Rolf |
| description | Sixty-six strains classified as
P. verrucosum based on morphological criteria were characterized by molecular methods like RAPD, AFLP and ITS sequencing. Two groups could be identified by RAPD and AFLP analyses. The two RAPD as well as the two AFLP groups were completely coincidental. Strains in the two groups differed in their ability to produce ochratoxin A, with group I containing mainly high producing strains, and group II containing moderate to non-producing strains. The strains from group I originate from foods, such as cheeses and meat products, while the strains from group II originate from plants. The ribosomal ITS15.8S-ITS2 sequences were similar, except for two single nucleotide exchanges in several strains of each group.
A chemotaxonomical analysis of some of the strains identified differences between the groups in secondary metabolite production. Strains from group I possessed the chemotype of
P. nordicum and strains from group II that of
P. verrucosum. The differences at the RAPD and AFLP level, which parallel the chemotypic differences, are consistent with the recent reclassification of ochratoxin A producing penicillia to be either
P. verrucosum or
P. nordicum. The homolgy between the ITS sequences however indicates phylogenetic relationship between the two species. |
| doi_str_mv | 10.1078/0723-2020-00094 |
| format | Article |
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P. verrucosum based on morphological criteria were characterized by molecular methods like RAPD, AFLP and ITS sequencing. Two groups could be identified by RAPD and AFLP analyses. The two RAPD as well as the two AFLP groups were completely coincidental. Strains in the two groups differed in their ability to produce ochratoxin A, with group I containing mainly high producing strains, and group II containing moderate to non-producing strains. The strains from group I originate from foods, such as cheeses and meat products, while the strains from group II originate from plants. The ribosomal ITS15.8S-ITS2 sequences were similar, except for two single nucleotide exchanges in several strains of each group.
A chemotaxonomical analysis of some of the strains identified differences between the groups in secondary metabolite production. Strains from group I possessed the chemotype of
P. nordicum and strains from group II that of
P. verrucosum. The differences at the RAPD and AFLP level, which parallel the chemotypic differences, are consistent with the recent reclassification of ochratoxin A producing penicillia to be either
P. verrucosum or
P. nordicum. The homolgy between the ITS sequences however indicates phylogenetic relationship between the two species.</description><identifier>ISSN: 0723-2020</identifier><identifier>EISSN: 1618-0984</identifier><identifier>DOI: 10.1078/0723-2020-00094</identifier><identifier>PMID: 12086192</identifier><identifier>CODEN: SAMIDF</identifier><language>eng</language><publisher>Jena: Elsevier GmbH</publisher><subject>Action of physical and chemical agents ; AFLP typing ; Base Sequence ; Biological and medical sciences ; chemotyping ; Citrinin - analysis ; DNA Primers ; DNA, Ribosomal Spacer - analysis ; Fundamental and applied biological sciences. Psychology ; Genetic Variation ; ITS sequences ; Microbiology ; Miscellaneous ; Molecular Sequence Data ; Mycology ; ochratoxin ; Ochratoxins - analysis ; Ochratoxins - biosynthesis ; Penicillium - classification ; Penicillium - genetics ; Penicillium - isolation & purification ; Penicillium - metabolism ; Penicillium nordicum ; Penicillium verrucosum ; Phylogeny ; Polymorphism, Restriction Fragment Length ; Random Amplified Polymorphic DNA Technique - methods ; RAPD typing ; Sequence Alignment ; Virology</subject><ispartof>Systematic and applied microbiology, 2002-04, Vol.25 (1), p.74-83</ispartof><rights>2002 Urban & Fischer Verlag</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c373t-f616b6b9efe4f6dce9894ab472cab309497b7b0cbc4a7724bbe7f1b5b7623add3</citedby><cites>FETCH-LOGICAL-c373t-f616b6b9efe4f6dce9894ab472cab309497b7b0cbc4a7724bbe7f1b5b7623add3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0723202004700892$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13692316$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12086192$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Castella, Gemma</creatorcontrib><creatorcontrib>Ostenfeld Larsen, Thomas</creatorcontrib><creatorcontrib>Cabanes, Javier</creatorcontrib><creatorcontrib>Schmidt, Holger</creatorcontrib><creatorcontrib>Alboresi, Alessandro</creatorcontrib><creatorcontrib>Niessen, Ludwig</creatorcontrib><creatorcontrib>Färber, Paul</creatorcontrib><creatorcontrib>Geisen, Rolf</creatorcontrib><title>Molecular Characterization of Ochratoxin A Producing Strains of the Genus Penicillium</title><title>Systematic and applied microbiology</title><addtitle>Syst Appl Microbiol</addtitle><description>Sixty-six strains classified as
P. verrucosum based on morphological criteria were characterized by molecular methods like RAPD, AFLP and ITS sequencing. Two groups could be identified by RAPD and AFLP analyses. The two RAPD as well as the two AFLP groups were completely coincidental. Strains in the two groups differed in their ability to produce ochratoxin A, with group I containing mainly high producing strains, and group II containing moderate to non-producing strains. The strains from group I originate from foods, such as cheeses and meat products, while the strains from group II originate from plants. The ribosomal ITS15.8S-ITS2 sequences were similar, except for two single nucleotide exchanges in several strains of each group.
A chemotaxonomical analysis of some of the strains identified differences between the groups in secondary metabolite production. Strains from group I possessed the chemotype of
P. nordicum and strains from group II that of
P. verrucosum. The differences at the RAPD and AFLP level, which parallel the chemotypic differences, are consistent with the recent reclassification of ochratoxin A producing penicillia to be either
P. verrucosum or
P. nordicum. The homolgy between the ITS sequences however indicates phylogenetic relationship between the two species.</description><subject>Action of physical and chemical agents</subject><subject>AFLP typing</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>chemotyping</subject><subject>Citrinin - analysis</subject><subject>DNA Primers</subject><subject>DNA, Ribosomal Spacer - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Variation</subject><subject>ITS sequences</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Molecular Sequence Data</subject><subject>Mycology</subject><subject>ochratoxin</subject><subject>Ochratoxins - analysis</subject><subject>Ochratoxins - biosynthesis</subject><subject>Penicillium - classification</subject><subject>Penicillium - genetics</subject><subject>Penicillium - isolation & purification</subject><subject>Penicillium - metabolism</subject><subject>Penicillium nordicum</subject><subject>Penicillium verrucosum</subject><subject>Phylogeny</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Random Amplified Polymorphic DNA Technique - methods</subject><subject>RAPD typing</subject><subject>Sequence Alignment</subject><subject>Virology</subject><issn>0723-2020</issn><issn>1618-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kDFPwzAQRi0EoqUwsyEvjAHbCXY8VhUUpKJWgs6W7ZypUepUdoKAX09KKzox3XDvO933ELqk5IYSUd4SwfKMEUYyQogsjtCQclpmRJbFMRr-bQfoLKV3QmghOT1FA8pIyalkQ7R8bmqwXa0jnqx01LaF6L9165uAG4fndhV123z6gMd4EZuqsz684Zc2ah_SlmhXgKcQuoQXELz1de279Tk6cbpOcLGfI7R8uH-dPGaz-fRpMp5lNhd5mzlOueFGgoPC8cqCLGWhTSGY1Sbv-0hhhCHW2EILwQpjQDhq7ozgLNdVlY_Q7e6ujU1KEZzaRL_W8UtRoraC1FaB2ipQv4L6xNUusenMGqoDvzfSA9d7QCeraxd1sD4duJz3EOU9J3cc9P0-PESVrIdgofIRbKuqxv_7xA_-y4Dk</recordid><startdate>20020401</startdate><enddate>20020401</enddate><creator>Castella, Gemma</creator><creator>Ostenfeld Larsen, Thomas</creator><creator>Cabanes, Javier</creator><creator>Schmidt, Holger</creator><creator>Alboresi, Alessandro</creator><creator>Niessen, Ludwig</creator><creator>Färber, Paul</creator><creator>Geisen, Rolf</creator><general>Elsevier GmbH</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20020401</creationdate><title>Molecular Characterization of Ochratoxin A Producing Strains of the Genus Penicillium</title><author>Castella, Gemma ; Ostenfeld Larsen, Thomas ; Cabanes, Javier ; Schmidt, Holger ; Alboresi, Alessandro ; Niessen, Ludwig ; Färber, Paul ; Geisen, Rolf</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c373t-f616b6b9efe4f6dce9894ab472cab309497b7b0cbc4a7724bbe7f1b5b7623add3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Action of physical and chemical agents</topic><topic>AFLP typing</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>chemotyping</topic><topic>Citrinin - analysis</topic><topic>DNA Primers</topic><topic>DNA, Ribosomal Spacer - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Variation</topic><topic>ITS sequences</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Molecular Sequence Data</topic><topic>Mycology</topic><topic>ochratoxin</topic><topic>Ochratoxins - analysis</topic><topic>Ochratoxins - biosynthesis</topic><topic>Penicillium - classification</topic><topic>Penicillium - genetics</topic><topic>Penicillium - isolation & purification</topic><topic>Penicillium - metabolism</topic><topic>Penicillium nordicum</topic><topic>Penicillium verrucosum</topic><topic>Phylogeny</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Random Amplified Polymorphic DNA Technique - methods</topic><topic>RAPD typing</topic><topic>Sequence Alignment</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Castella, Gemma</creatorcontrib><creatorcontrib>Ostenfeld Larsen, Thomas</creatorcontrib><creatorcontrib>Cabanes, Javier</creatorcontrib><creatorcontrib>Schmidt, Holger</creatorcontrib><creatorcontrib>Alboresi, Alessandro</creatorcontrib><creatorcontrib>Niessen, Ludwig</creatorcontrib><creatorcontrib>Färber, Paul</creatorcontrib><creatorcontrib>Geisen, Rolf</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Systematic and applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Castella, Gemma</au><au>Ostenfeld Larsen, Thomas</au><au>Cabanes, Javier</au><au>Schmidt, Holger</au><au>Alboresi, Alessandro</au><au>Niessen, Ludwig</au><au>Färber, Paul</au><au>Geisen, Rolf</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Characterization of Ochratoxin A Producing Strains of the Genus Penicillium</atitle><jtitle>Systematic and applied microbiology</jtitle><addtitle>Syst Appl Microbiol</addtitle><date>2002-04-01</date><risdate>2002</risdate><volume>25</volume><issue>1</issue><spage>74</spage><epage>83</epage><pages>74-83</pages><issn>0723-2020</issn><eissn>1618-0984</eissn><coden>SAMIDF</coden><abstract>Sixty-six strains classified as
P. verrucosum based on morphological criteria were characterized by molecular methods like RAPD, AFLP and ITS sequencing. Two groups could be identified by RAPD and AFLP analyses. The two RAPD as well as the two AFLP groups were completely coincidental. Strains in the two groups differed in their ability to produce ochratoxin A, with group I containing mainly high producing strains, and group II containing moderate to non-producing strains. The strains from group I originate from foods, such as cheeses and meat products, while the strains from group II originate from plants. The ribosomal ITS15.8S-ITS2 sequences were similar, except for two single nucleotide exchanges in several strains of each group.
A chemotaxonomical analysis of some of the strains identified differences between the groups in secondary metabolite production. Strains from group I possessed the chemotype of
P. nordicum and strains from group II that of
P. verrucosum. The differences at the RAPD and AFLP level, which parallel the chemotypic differences, are consistent with the recent reclassification of ochratoxin A producing penicillia to be either
P. verrucosum or
P. nordicum. The homolgy between the ITS sequences however indicates phylogenetic relationship between the two species.</abstract><cop>Jena</cop><pub>Elsevier GmbH</pub><pmid>12086192</pmid><doi>10.1078/0723-2020-00094</doi><tpages>10</tpages></addata></record> |
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| subjects | Action of physical and chemical agents AFLP typing Base Sequence Biological and medical sciences chemotyping Citrinin - analysis DNA Primers DNA, Ribosomal Spacer - analysis Fundamental and applied biological sciences. Psychology Genetic Variation ITS sequences Microbiology Miscellaneous Molecular Sequence Data Mycology ochratoxin Ochratoxins - analysis Ochratoxins - biosynthesis Penicillium - classification Penicillium - genetics Penicillium - isolation & purification Penicillium - metabolism Penicillium nordicum Penicillium verrucosum Phylogeny Polymorphism, Restriction Fragment Length Random Amplified Polymorphic DNA Technique - methods RAPD typing Sequence Alignment Virology |
| title | Molecular Characterization of Ochratoxin A Producing Strains of the Genus Penicillium |
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