Evaluation of Two-dimensional Differential Gel Electrophoresis for Proteomic Expression Analysis of a Model Breast Cancer Cell System

The technique of fluorescent two-dimensional (2D) difference gel electrophoresis for differential protein expression analysis has been evaluated using a model breast cancer cell system of ErbB-2 overexpression. Labeling of paired cell lysate samples with N -hydroxy succinimidyl ester-derivatives of...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Molecular & cellular proteomics 2002-02, Vol.1 (2), p.91-98
Hauptverfasser:	Gharbi, Severine, Gaffney, Piers, Yang, Alice, Zvelebil, Marketa J, Cramer, Rainer, Waterfield, Michael D, Timms, John F
Format:	Artikel
Sprache:	eng
Schlagworte:	Breast Neoplasms - genetics Electrophoresis, Gel, Two-Dimensional - methods Electrophoresis, Gel, Two-Dimensional - statistics & numerical data Fluorescent Dyes Gene Expression - drug effects Genes, erbB-2 Neoplasm Proteins - genetics Neoplasm Proteins - isolation & purification Neuregulin-1 - pharmacology Proteome Reproducibility of Results Sensitivity and Specificity Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Staining and Labeling Tumor Cells, Cultured
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!