Catalytic Mechanism of Nitrile Hydratase Proposed by Time-resolved X-ray Crystallography Using a Novel Substrate, tert-Butylisonitrile

Nitrile hydratases (NHases) have an unusual iron or cobalt catalytic center with two oxidized cysteine ligands, cysteine-sulfinic acid and cysteine-sulfenic acid, catalyzing the hydration of nitriles to amides. Recently, we found that the NHase of Rhodococcus erythropolis N771 exhibited an additiona...

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Veröffentlicht in:	The Journal of biological chemistry 2008-12, Vol.283 (52), p.36617-36623
Hauptverfasser:	Hashimoto, Koichi, Suzuki, Hiroyuki, Taniguchi, Kayoko, Noguchi, Takumi, Yohda, Masafumi, Odaka, Masafumi
Format:	Artikel
Sprache:	eng
Schlagworte:	Carbon - chemistry Carbon Monoxide - chemistry Catalysis Crystallography, X-Ray - methods Electrons Equipment Design Hydro-Lyases - chemistry Iron - chemistry Kinetics Molecular Conformation Nitriles - chemistry Nitrogen - chemistry Rhodococcus - metabolism Solvents - chemistry Spectroscopy, Fourier Transform Infrared
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