Role of a Carboxylesterase in Herbicide Bioactivation in Arabidopsis thaliana

Arabidopsis thaliana contains multiple carboxyesterases (AtCXEs) with activities toward xenobiotics, including herbicide esters that are activated to their phytotoxic acids upon hydrolysis. On the basis of their susceptibility to inhibition by organophosphates, these AtCXEs are all serine hydrolases...

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Veröffentlicht in:The Journal of biological chemistry 2007-07, Vol.282 (29), p.21460-21466
Hauptverfasser: Gershater, Markus C., Cummins, Ian, Edwards, Robert
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container_title The Journal of biological chemistry
container_volume 282
creator Gershater, Markus C.
Cummins, Ian
Edwards, Robert
description Arabidopsis thaliana contains multiple carboxyesterases (AtCXEs) with activities toward xenobiotics, including herbicide esters that are activated to their phytotoxic acids upon hydrolysis. On the basis of their susceptibility to inhibition by organophosphates, these AtCXEs are all serine hydrolases. Using a trifunctional probe bearing a fluorophosphonate together with biotin and rhodamine to facilitate detection and recovery, four dominant serine hydrolases were identified in the proteome of Arabidopsis. Using a combination of protein purification, capture with the trifunctional probe and proteomics, one of these hydrolases, AtCXE12, was shown to be the major carboxyesterase responsible for hydrolyzing the pro-herbicide methyl-2,4-dichlorophenoxyacetate (2,4-D-methyl) to the phytotoxic acid 2,4-dichlorophenoxyacetic acid. Recombinant expression of the other identified hydrolases showed that AtCXE12 was unique in hydrolyzing 2,4-D-methyl. To determine the importance of AtCXE12 in herbicide metabolism and efficacy, the respective tDNA knock-out (atcxe12) plants were characterized and shown to lack expression of AtCXE12 and have greatly reduced levels of 2,4-D-methyl-hydrolyzing activity. Young atcxe12 seedlings were less sensitive than wild type plants to 2,4-D-methyl, confirming a role for the enzyme in herbicide bioactivation in Arabidopsis.
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source MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection
subjects 2,4-Dichlorophenoxyacetic Acid - chemistry
Arabidopsis - enzymology
Carboxylesterase - chemistry
Cloning, Molecular
Gene Expression Regulation, Plant
Herbicides - chemistry
Hydrolases - chemistry
Hydrolysis
Models, Chemical
Organophosphonates - chemistry
Proteins - chemistry
Seedlings
Xenobiotics - chemistry
title Role of a Carboxylesterase in Herbicide Bioactivation in Arabidopsis thaliana
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