Characterization of Two Mosquito STATs, AaSTAT and CtSTAT
Two mosquito STATs, Aa STAT and Ct STAT, have been cloned from Aedes albopictus and Culex tritaeniorhynchus mosquitoes, respectively. These two STATs are more similar to those of Drosophila , Anopheles , and mammalian STAT5 in the DNA binding and Src homology 2 domains. The mRNA transcripts are expr...
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| Veröffentlicht in: | The Journal of biological chemistry 2004-01, Vol.279 (5), p.3308-3317 |
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| container_title | The Journal of biological chemistry |
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| creator | Lin, Chang-Chi Chou, Chih-Ming Hsu, Ya-Li Lien, Jih-Ching Wang, Yu-Ming Chen, Shui-Tsung Tsai, Shu-Chuan Hsiao, Pei-Wen Huang, Chang-Jen |
| description | Two mosquito STATs, Aa STAT and Ct STAT, have been cloned from Aedes albopictus and Culex tritaeniorhynchus mosquitoes, respectively. These two STATs are more similar to those of Drosophila , Anopheles , and mammalian STAT5 in the DNA binding and Src homology 2 domains. The mRNA transcripts are expressed at all developmental
stages, and the proteins are present predominantly at the pupal and adult stages in both mosquitoes. Stimulation with lipopolysaccharide
resulted in an increase of tyrosine phosphorylation and DNA binding activity of Aa STAT and Ct STAT as well as an increase of luciferase activity of a reporter gene containing Drosophila STAT binding motif in mosquito C6/36 cells. After being infected with Japanese encephalitis virus, nuclear extracts of C6/36
cells revealed a decrease of tyrosine phosphorylation and DNA binding activity of Aa STAT which could be restored by sodium orthovanadate treatment. Taking all of the data together, this is the first report
to clone and characterize two mosquito STATs with 81% identity and to demonstrate a different response of tyrosine phosphorylation
and DNA binding of these two STATs by lipopolysaccharide treatment and by Japanese encephalitis virus infection. |
| doi_str_mv | 10.1074/jbc.M309749200 |
| format | Article |
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stages, and the proteins are present predominantly at the pupal and adult stages in both mosquitoes. Stimulation with lipopolysaccharide
resulted in an increase of tyrosine phosphorylation and DNA binding activity of Aa STAT and Ct STAT as well as an increase of luciferase activity of a reporter gene containing Drosophila STAT binding motif in mosquito C6/36 cells. After being infected with Japanese encephalitis virus, nuclear extracts of C6/36
cells revealed a decrease of tyrosine phosphorylation and DNA binding activity of Aa STAT which could be restored by sodium orthovanadate treatment. Taking all of the data together, this is the first report
to clone and characterize two mosquito STATs with 81% identity and to demonstrate a different response of tyrosine phosphorylation
and DNA binding of these two STATs by lipopolysaccharide treatment and by Japanese encephalitis virus infection.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.M309749200</identifier><identifier>PMID: 14607839</identifier><language>eng</language><publisher>American Society for Biochemistry and Molecular Biology</publisher><ispartof>The Journal of biological chemistry, 2004-01, Vol.279 (5), p.3308-3317</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2170-3d853f86e7d8f34c68eec6d5bce251154cb1267d7fa9a3d261480e08b29909d33</citedby><cites>FETCH-LOGICAL-c2170-3d853f86e7d8f34c68eec6d5bce251154cb1267d7fa9a3d261480e08b29909d33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Lin, Chang-Chi</creatorcontrib><creatorcontrib>Chou, Chih-Ming</creatorcontrib><creatorcontrib>Hsu, Ya-Li</creatorcontrib><creatorcontrib>Lien, Jih-Ching</creatorcontrib><creatorcontrib>Wang, Yu-Ming</creatorcontrib><creatorcontrib>Chen, Shui-Tsung</creatorcontrib><creatorcontrib>Tsai, Shu-Chuan</creatorcontrib><creatorcontrib>Hsiao, Pei-Wen</creatorcontrib><creatorcontrib>Huang, Chang-Jen</creatorcontrib><title>Characterization of Two Mosquito STATs, AaSTAT and CtSTAT</title><title>The Journal of biological chemistry</title><description>Two mosquito STATs, Aa STAT and Ct STAT, have been cloned from Aedes albopictus and Culex tritaeniorhynchus mosquitoes, respectively. These two STATs are more similar to those of Drosophila , Anopheles , and mammalian STAT5 in the DNA binding and Src homology 2 domains. The mRNA transcripts are expressed at all developmental
stages, and the proteins are present predominantly at the pupal and adult stages in both mosquitoes. Stimulation with lipopolysaccharide
resulted in an increase of tyrosine phosphorylation and DNA binding activity of Aa STAT and Ct STAT as well as an increase of luciferase activity of a reporter gene containing Drosophila STAT binding motif in mosquito C6/36 cells. After being infected with Japanese encephalitis virus, nuclear extracts of C6/36
cells revealed a decrease of tyrosine phosphorylation and DNA binding activity of Aa STAT which could be restored by sodium orthovanadate treatment. Taking all of the data together, this is the first report
to clone and characterize two mosquito STATs with 81% identity and to demonstrate a different response of tyrosine phosphorylation
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stages, and the proteins are present predominantly at the pupal and adult stages in both mosquitoes. Stimulation with lipopolysaccharide
resulted in an increase of tyrosine phosphorylation and DNA binding activity of Aa STAT and Ct STAT as well as an increase of luciferase activity of a reporter gene containing Drosophila STAT binding motif in mosquito C6/36 cells. After being infected with Japanese encephalitis virus, nuclear extracts of C6/36
cells revealed a decrease of tyrosine phosphorylation and DNA binding activity of Aa STAT which could be restored by sodium orthovanadate treatment. Taking all of the data together, this is the first report
to clone and characterize two mosquito STATs with 81% identity and to demonstrate a different response of tyrosine phosphorylation
and DNA binding of these two STATs by lipopolysaccharide treatment and by Japanese encephalitis virus infection.</abstract><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>14607839</pmid><doi>10.1074/jbc.M309749200</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| title | Characterization of Two Mosquito STATs, AaSTAT and CtSTAT |
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