Exploratory and Confirmatory Gene Expression Profiling of mac1Δ
Exploratory outlier identification methods and confirmatory gene expression studies showed induction of the iron regulon in Saccharomyces cerevisiae lacking Mac1p, a copper-responsive transcription factor. The Aft1p/Aft2p binding motif was the most discriminating motif between up- and down-regulated...
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| Veröffentlicht in: | The Journal of biological chemistry 2004-02, Vol.279 (6), p.4450-4458 |
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| container_issue | 6 |
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| container_title | The Journal of biological chemistry |
| container_volume | 279 |
| creator | De Freitas, Jeane Maria Kim, Jeung Hyoun Poynton, Helen Su, Trent Wintz, Henri Fox, Tama Holman, Patty Loguinov, Alex Keles, Sunduz van der Laan, Mark Vulpe, Chris |
| description | Exploratory outlier identification methods and confirmatory gene expression studies showed induction of the iron regulon in Saccharomyces cerevisiae lacking Mac1p, a copper-responsive transcription factor. The Aft1p/Aft2p binding motif was the most discriminating motif between up- and down-regulated genes, and we identified new genes potentially regulated by Aft1p/Aft2p. In addition, multiple genes encoding proteins containing Fe-S clusters were down-regulated suggesting metabolic reorganization to conserve iron in mac1Δ. Null mutants of each of the differentially expressed genes were characterized for copper- or iron-related phenotypes. New or additional support for a role in copper and iron homeostasis is provided in this study for the gene products of AKR1, MRS4, PCA1, SSU1, TIS11, YBR047W, YHL035C, YHR045W, YLR047C, YLR126C, and YTP1. |
| doi_str_mv | 10.1074/jbc.M212308200 |
| format | Article |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| title | Exploratory and Confirmatory Gene Expression Profiling of mac1Δ |
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