Creating Directed Double-strand Breaks with the Ref Protein

The bacteriophage P1-encoded Ref protein enhances RecA-dependent recombination in vivo by an unknown mechanism. We demonstrate that Ref is a new type of enzyme; that is, a RecA-dependent nuclease. Ref binds to ss- and dsDNA but does not cleave any DNA substrate until RecA protein and ATP are added t...

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Veröffentlicht in:	The Journal of biological chemistry 2011-03, Vol.286 (10), p.8240-8251
Hauptverfasser:	Gruenig, Marielle C., Lu, Duo, Won, Sang Joon, Dulberger, Charles L., Manlick, Angela J., Keck, James L., Cox, Michael M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriophage DNA DNA Recombination DNA Repair HNH Nuclease Nucleic Acid Enzymology RecA Ref
Online-Zugang:	Volltext
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