Purification of Heterotrimeric G Protein α Subunits by GST-Ric-8 Association

Ric-8A and Ric-8B are nonreceptor G protein guanine nucleotide exchange factors that collectively bind the four subfamilies of G protein α subunits. Co-expression of Gα subunits with Ric-8A or Ric-8B in HEK293 cells or insect cells greatly promoted Gα protein expression. We exploited these character...

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Veröffentlicht in:	The Journal of biological chemistry 2011-01, Vol.286 (4), p.2625-2635
Hauptverfasser:	Chan, PuiYee, Gabay, Meital, Wright, Forrest A., Kan, Wei, Oner, Sukru S., Lanier, Stephen M., Smrcka, Alan V., Blumer, Joe B., Tall, Gregory G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenylate Cyclase (Adenylyl Cyclase) G Proteins Guanine Nucleotide Exchange Factor Myristoylation Phospholipase C Protein Purification Ric-8 Signal Transduction
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