Rapid Purification and High Sensitivity Analysis of Heparan Sulfate from Cells and Tissues

Studies on glycosaminoglycans and proteoglycans (PGs) have been hampered by difficulties in isolation and analysis by traditional methods that are laborious and lack sensitivity and throughput. Here we demonstrate a simple method for rapid isolation of proteoglycans (RIP) employing phenol/guanidine/...

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Veröffentlicht in:The Journal of biological chemistry 2009-09, Vol.284 (38), p.25714-25722
Hauptverfasser: Guimond, Scott E., Puvirajesinghe, Tania M., Skidmore, Mark A., Kalus, Ina, Dierks, Thomas, Yates, Edwin A., Turnbull, Jeremy E.
Format: Artikel
Sprache:eng
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