Asymmetric Photocross-linking Pattern of Restriction EndonucleaseEcoRII to the DNA Recognition Sequence

The EcoRII homodimer engages two of its recognition sequences (5′-CCWGG) simultaneously and is therefore a type IIE restriction endonuclease. To identify the amino acids ofEcoRII that interact specifically with the recognition sequence, we photocross-linked EcoRII with oligonucleotide substrates tha...

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Veröffentlicht in:	The Journal of biological chemistry 2002-04, Vol.277 (16), p.14288-14293
Hauptverfasser:	Mücke, Merlind, Pingoud, Vera, Grelle, Gerlinde, Kraft, Regine, Krüger, Detlev H., Reuter, Monika
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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