Conserved Walker A Ser Residues in the Catalytic Sites of P-glycoprotein Are Critical for Catalysis and Involved Primarily at the Transition State Step

P-glycoprotein mutants S430A/T and S1073A/T, affecting conserved Walker A Ser residues, were characterized to elucidate molecular roles of the Ser and functioning of the two P-glycoprotein catalytic sites. Results showed the Ser-OH is critical for MgATPase activity and formation of the normal transi...

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Veröffentlicht in:	The Journal of biological chemistry 2000-08, Vol.275 (32), p.25031-25038
Hauptverfasser:	Urbatsch, Ina L., Gimi, Khursheed, Wilke-Mounts, Susan, Senior, Alan E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino Acid Substitution Animals ATP Binding Cassette Transporter, Subfamily B, Member 1 - chemistry ATP Binding Cassette Transporter, Subfamily B, Member 1 - metabolism Ca(2+) Mg(2+)-ATPase - antagonists & inhibitors Ca(2+) Mg(2+)-ATPase - metabolism Catalysis Catalytic Domain Cloning, Molecular Consensus Sequence Conserved Sequence Kinetics Magnesium - metabolism Mammals Mutagenesis, Site-Directed Pichia Point Mutation Serine Vanadates - pharmacology
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