The Aglycone Specificity-determining Sites Are Different in 2,4-Dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA)-glucosidase (Maize β-Glucosidase) and Dhurrinase (Sorghum β-Glucosidase)

The maize β-glucosidase isozyme Glu1 hydrolyzes a broad spectrum of substrates in addition to its natural substrate DIMBOAGlc (2-O-β-d-glucopyranosyl-4-hydroxy-7-methoxy-1,4-benzoxazin-3-one), whereas the sorghum β-glucosidase isozyme Dhr1 hydrolyzes exclusively its natural substrate dhurrin (p-hydr...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	The Journal of biological chemistry 2000-06, Vol.275 (26), p.20002-20011
Hauptverfasser:	Cicek, Muzaffer, Blanchard, David, Bevan, David R., Esen, Asim
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence beta-Glucosidase - chemistry beta-Glucosidase - genetics beta-Glucosidase - metabolism Binding Sites Chromatography, Thin Layer Electrophoresis, Polyacrylamide Gel Hydrolysis Isoenzymes - chemistry Kinetics Models, Chemical Models, Molecular Molecular Sequence Data Mutagenesis Nitriles - metabolism Plant Proteins Poaceae - enzymology Protein Structure, Tertiary Recombinant Proteins - chemistry Sequence Homology, Amino Acid Substrate Specificity Zea mays - enzymology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!