Ingestion of Bacterially Expressed Double-Stranded RNA Inhibits Gene Expression in Planarians
Freshwater planarian flatworms are capable of regenerating complete organisms from tiny fragments of their bodies; the basis for this regenerative prowess is an experimentally accessible stem cell population that is present in the adult planarian. The study of these organisms, classic experimental m...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2003-09, Vol.100 (Suppl 1), p.11861-11865 |
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| creator | Newmark, Phillip A. Reddien, Peter W. Cebrià, Francesc Alvarado, Alejandro Sánchez |
| description | Freshwater planarian flatworms are capable of regenerating complete organisms from tiny fragments of their bodies; the basis for this regenerative prowess is an experimentally accessible stem cell population that is present in the adult planarian. The study of these organisms, classic experimental models for investigating metazoan regeneration, has been revitalized by the application of modern molecular biological approaches. The identification of thousands of unique planarian ESTs, coupled with large-scale whole-mount in situ hybridization screens, and the ability to inhibit planarian gene expression through double-stranded RNA-mediated genetic interference, provide a wealth of tools for studying the molecular mechanisms that regulate tissue regeneration and stem cell biology in these organisms. Here we show that, as in Caenorhabditis elegans, ingestion of bacterially expressed double-stranded RNA can inhibit gene expression in planarians. This inhibition persists throughout the process of regeneration, allowing phenotypes with disrupted regenerative patterning to be identified. These results pave the way for large-scale screens for genes involved in regenerative processes. |
| doi_str_mv | 10.1073/pnas.1834205100 |
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The study of these organisms, classic experimental models for investigating metazoan regeneration, has been revitalized by the application of modern molecular biological approaches. The identification of thousands of unique planarian ESTs, coupled with large-scale whole-mount in situ hybridization screens, and the ability to inhibit planarian gene expression through double-stranded RNA-mediated genetic interference, provide a wealth of tools for studying the molecular mechanisms that regulate tissue regeneration and stem cell biology in these organisms. Here we show that, as in Caenorhabditis elegans, ingestion of bacterially expressed double-stranded RNA can inhibit gene expression in planarians. This inhibition persists throughout the process of regeneration, allowing phenotypes with disrupted regenerative patterning to be identified. 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These results pave the way for large-scale screens for genes involved in regenerative processes.</description><subject>Animals</subject><subject>Axons</subject><subject>Bacteria</subject><subject>Colloquium Papers</subject><subject>Double stranded RNA</subject><subject>Escherichia coli - genetics</subject><subject>Gene Expression</subject><subject>Genetic screening</subject><subject>Microinjections</subject><subject>Neurons</subject><subject>Phenotype</subject><subject>Phenotypes</subject><subject>Photoreceptors</subject><subject>Planarians - genetics</subject><subject>Planarians - physiology</subject><subject>Regeneration</subject><subject>Regeneration - genetics</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA - administration & dosage</subject><subject>RNA - genetics</subject><subject>RNA Interference</subject><subject>RNA, Double-Stranded - administration & dosage</subject><subject>RNA, Double-Stranded - genetics</subject><subject>Stem cells</subject><subject>Worms</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAUxC0EotvCmQtCEVK5pX3-iu1DD6WUslIFCHpFltdxWq-ydrATRP97HO3SBS6cLMu_Gc97g9ALDCcYBD0dgsknWFJGgGOAR2iBQeG6YQoeowUAEbVkhB2gw5zXAKC4hKfoABOFRWEW6Nsy3Lo8-hiq2FVvjR1d8qbv76vLn0NyObu2ehenVe_qr2MyoS33Lx_Pq2W48ys_5urKBfebnV18qD73JpjiEvIz9KQzfXbPd-cRunl_eXPxob7-dLW8OL-uLW_oWCtOSkowRipjFWuAtdBaB4J1LVEtFZK1xABrWmctVZJxC6vOsIZY0glJj9DZ1naYVhtXlKFE7fWQ_Makex2N13-_BH-nb-MPTYGBUkX_ZqdP8ftU1qE3PlvXlzlcnLIWXBAuufwviKWkhJOmgK__AddxSqHsQBPADCghuECnW8immHNy3UNiDHquV8_16n29RfHqz0H3_K7PAhzvgFm5twOdp2HoNdYYy2b--uWWW-cxpgeQYiYopvQXnGK3jg</recordid><startdate>20030930</startdate><enddate>20030930</enddate><creator>Newmark, Phillip A.</creator><creator>Reddien, Peter W.</creator><creator>Cebrià, Francesc</creator><creator>Alvarado, Alejandro Sánchez</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20030930</creationdate><title>Ingestion of Bacterially Expressed Double-Stranded RNA Inhibits Gene Expression in Planarians</title><author>Newmark, Phillip A. ; 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| subjects | Animals Axons Bacteria Colloquium Papers Double stranded RNA Escherichia coli - genetics Gene Expression Genetic screening Microinjections Neurons Phenotype Phenotypes Photoreceptors Planarians - genetics Planarians - physiology Regeneration Regeneration - genetics Ribonucleic acid RNA RNA - administration & dosage RNA - genetics RNA Interference RNA, Double-Stranded - administration & dosage RNA, Double-Stranded - genetics Stem cells Worms |
| title | Ingestion of Bacterially Expressed Double-Stranded RNA Inhibits Gene Expression in Planarians |
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