Genomewide Insertional Mutagenesis in Streptomyces coelicolor Reveals Additional Genes Involved in Morphological Differentiation

The filamentous soil bacterium Streptomyces coelicolor undergoes a complex cycle of morphological differentiation involving the formation of an aerial mycelium and the production of pigmented antibiotics. We have developed a procedure for generating insertional mutants of S. coelicolor based on in v...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 2000-08, Vol.97 (17), p.9642-9647
Hauptverfasser: Gehring, Amy M., Nodwell, Justin R., Beverley, Stephen M., Losick, Richard
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container_issue 17
container_start_page 9642
container_title Proceedings of the National Academy of Sciences - PNAS
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creator Gehring, Amy M.
Nodwell, Justin R.
Beverley, Stephen M.
Losick, Richard
description The filamentous soil bacterium Streptomyces coelicolor undergoes a complex cycle of morphological differentiation involving the formation of an aerial mycelium and the production of pigmented antibiotics. We have developed a procedure for generating insertional mutants of S. coelicolor based on in vitro transposition of a plasmid library of cloned S. coelicolor DNAs. The insertionally mutated library was introduced into S. coelicolor, and transposon insertions were recovered at widely scattered locations around the chromosome. Many of the insertions revealed previously uncharacterized genes, and several caused novel mutant phenotypes, such as altered pigment production, enhanced antibiotic sensitivity, delayed or impaired formation of aerial hyphae, and a block in spore formation. The sporulation mutant harbored an insertion in one of three adjacent genes that are apparently unique to Streptomyces but are each represented by at least 20 paralogs at dispersed locations in the chromosome. Individual members of the three families often are found grouped together in a characteristic arrangement, suggesting that they have a common function.
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subjects Amino Acid Sequence
Anti-Bacterial Agents - pharmacology
Bacteria
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - physiology
Base Sequence
Biological Sciences
Chromosomes
Chromosomes, Bacterial - genetics
DNA Transposable Elements - genetics
Genes
Genes, Bacterial - genetics
Genetic mutation
Genetic transposition
Genome, Bacterial
Hybridity
Microbial Sensitivity Tests
Microbiology
Molecular Sequence Data
Multigene Family - genetics
Mutagenesis, Insertional - genetics
Mutation
Nebramycin - analogs & derivatives
Nebramycin - pharmacology
Open reading frames
Phenotype
Phenotypes
Physical Chromosome Mapping
Pigments
Pigments, Biological - biosynthesis
Plasmids
Recombination, Genetic - genetics
Sequence Alignment
Spores, Bacterial - cytology
Spores, Bacterial - genetics
Spores, Bacterial - growth & development
Streptomyces - drug effects
Streptomyces - genetics
Streptomyces - growth & development
Streptomyces - metabolism
Streptomyces coelicolor
Transposons
title Genomewide Insertional Mutagenesis in Streptomyces coelicolor Reveals Additional Genes Involved in Morphological Differentiation
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