Extracardiac septum transversum/proepicardial endothelial cells pattern embryonic coronary arterio–venous connections
Recent reports suggest that mammalian embryonic coronary endothelium (CoE) originates from the sinus venosus and ventricular endocardium. However, the contribution of extracardiac cells to CoE is thought to be minor and nonsignificant for coronary formation. Using classic (Wt1Cre ) and previously un...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2016-01, Vol.113 (3), p.656-661 |
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| creator | Cano, Elena Carmona, Rita Ruiz-Villalba, Adrián Rojas, Anabel Chau, You-Ying Wagner, Kay D. Wagner, Nicole Hastie, Nicholas D. Muñoz-Chápuli, Ramón Pérez-Pomares, José M. |
| description | Recent reports suggest that mammalian embryonic coronary endothelium (CoE) originates from the sinus venosus and ventricular endocardium. However, the contribution of extracardiac cells to CoE is thought to be minor and nonsignificant for coronary formation. Using classic (Wt1Cre
) and previously undescribed (G2-Gata4Cre
) transgenic mouse models for the study of coronary vascular development, we show that extracardiac septum transversum/proepicardium (ST/PE)-derived endothelial cells are required for the formation of ventricular coronary arterio–venous vascular connections. Our results indicate that at least 20% of embryonic coronary arterial and capillary endothelial cells derive from the ST/PE compartment. Moreover, we show that conditional deletion of the ST/PE lineage-specific Wilms’ tumor suppressor gene (Wt1) in the ST/PE ofG2-Gata4Cre
mice and in the endothelium of Tie2Cre
mice disrupts embryonic coronary transmural patterning, leading to embryonic death. Taken together, our results demonstrate that ST/PE-derived endothelial cells contribute significantly to and are required for proper coronary vascular morphogenesis. |
| doi_str_mv | 10.1073/pnas.1509834113 |
| format | Article |
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) and previously undescribed (G2-Gata4Cre
) transgenic mouse models for the study of coronary vascular development, we show that extracardiac septum transversum/proepicardium (ST/PE)-derived endothelial cells are required for the formation of ventricular coronary arterio–venous vascular connections. Our results indicate that at least 20% of embryonic coronary arterial and capillary endothelial cells derive from the ST/PE compartment. Moreover, we show that conditional deletion of the ST/PE lineage-specific Wilms’ tumor suppressor gene (Wt1) in the ST/PE ofG2-Gata4Cre
mice and in the endothelium of Tie2Cre
mice disrupts embryonic coronary transmural patterning, leading to embryonic death. Taken together, our results demonstrate that ST/PE-derived endothelial cells contribute significantly to and are required for proper coronary vascular morphogenesis.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.1509834113</identifier><identifier>PMID: 26739565</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Animals ; Biological Sciences ; Biomarkers - metabolism ; Cell Lineage ; Cells ; Coronary Vessels - cytology ; Coronary Vessels - embryology ; Embryo, Mammalian - cytology ; Embryonic Development ; Endothelial Cells - cytology ; Enhancer Elements, Genetic - genetics ; Epithelial-Mesenchymal Transition ; GATA4 Transcription Factor - metabolism ; Gene Deletion ; Genes ; Genes, Reporter ; Green Fluorescent Proteins - metabolism ; Heart ; Heart Septum - cytology ; Integrases - metabolism ; Mice ; Models, Biological ; Myocytes, Cardiac - cytology ; Myocytes, Cardiac - metabolism ; Pericardium - cytology ; Phenotype ; Rodents ; Tumors ; WT1 Proteins - metabolism</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2016-01, Vol.113 (3), p.656-661</ispartof><rights>Volumes 1–89 and 106–113, copyright as a collective work only; author(s) retains copyright to individual articles</rights><rights>Copyright National Academy of Sciences Jan 19, 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c531t-79b3b6aa4764007ca906aad1bb5ca85ee20372f51309d3f1fbe259c93fa2375f3</citedby><cites>FETCH-LOGICAL-c531t-79b3b6aa4764007ca906aad1bb5ca85ee20372f51309d3f1fbe259c93fa2375f3</cites><orcidid>0000-0002-9686-473X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/113/3.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/26467442$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/26467442$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26739565$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cano, Elena</creatorcontrib><creatorcontrib>Carmona, Rita</creatorcontrib><creatorcontrib>Ruiz-Villalba, Adrián</creatorcontrib><creatorcontrib>Rojas, Anabel</creatorcontrib><creatorcontrib>Chau, You-Ying</creatorcontrib><creatorcontrib>Wagner, Kay D.</creatorcontrib><creatorcontrib>Wagner, Nicole</creatorcontrib><creatorcontrib>Hastie, Nicholas D.</creatorcontrib><creatorcontrib>Muñoz-Chápuli, Ramón</creatorcontrib><creatorcontrib>Pérez-Pomares, José M.</creatorcontrib><title>Extracardiac septum transversum/proepicardial endothelial cells pattern embryonic coronary arterio–venous connections</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Recent reports suggest that mammalian embryonic coronary endothelium (CoE) originates from the sinus venosus and ventricular endocardium. However, the contribution of extracardiac cells to CoE is thought to be minor and nonsignificant for coronary formation. Using classic (Wt1Cre
) and previously undescribed (G2-Gata4Cre
) transgenic mouse models for the study of coronary vascular development, we show that extracardiac septum transversum/proepicardium (ST/PE)-derived endothelial cells are required for the formation of ventricular coronary arterio–venous vascular connections. Our results indicate that at least 20% of embryonic coronary arterial and capillary endothelial cells derive from the ST/PE compartment. Moreover, we show that conditional deletion of the ST/PE lineage-specific Wilms’ tumor suppressor gene (Wt1) in the ST/PE ofG2-Gata4Cre
mice and in the endothelium of Tie2Cre
mice disrupts embryonic coronary transmural patterning, leading to embryonic death. Taken together, our results demonstrate that ST/PE-derived endothelial cells contribute significantly to and are required for proper coronary vascular morphogenesis.</description><subject>Animals</subject><subject>Biological Sciences</subject><subject>Biomarkers - metabolism</subject><subject>Cell Lineage</subject><subject>Cells</subject><subject>Coronary Vessels - cytology</subject><subject>Coronary Vessels - embryology</subject><subject>Embryo, Mammalian - cytology</subject><subject>Embryonic Development</subject><subject>Endothelial Cells - cytology</subject><subject>Enhancer Elements, Genetic - genetics</subject><subject>Epithelial-Mesenchymal Transition</subject><subject>GATA4 Transcription Factor - metabolism</subject><subject>Gene Deletion</subject><subject>Genes</subject><subject>Genes, Reporter</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Heart</subject><subject>Heart Septum - cytology</subject><subject>Integrases - metabolism</subject><subject>Mice</subject><subject>Models, Biological</subject><subject>Myocytes, Cardiac - cytology</subject><subject>Myocytes, Cardiac - metabolism</subject><subject>Pericardium - cytology</subject><subject>Phenotype</subject><subject>Rodents</subject><subject>Tumors</subject><subject>WT1 Proteins - metabolism</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkc1u1DAUhS0EotPCmhUoEut0_O94g4SqUpAqsWnXluPc0IwSO9jOQHe8Q9-wT4JHM0zLyj_nu8fHOgi9I_icYMXWs7fpnAisG8YJYS_QimBNask1folWGFNVN5zyE3Sa0gZjrEWDX6MTKhXTQooV-nX5O0frbOwG66oEc16mqtz4tIWYlmk9xwDzsAfGCnwX8h2Mu72DcUzVbHOG6CuY2ngf_OAqF2LwNt5XNhZlCI9_Hrbgw5KK4j24PASf3qBXvR0TvD2sZ-j2y-XNxdf6-vvVt4vP17UTjORa6Za10lquJMdYOatxOXWkbYWzjQCgmCnaC8Kw7lhP-hao0E6z3lKmRM_O0Ke977y0E3QOfPncaOY4TCWiCXYw_yt-uDM_wtZwRQVvZDH4eDCI4ecCKZtNWKIvmQ1RkijOGqoKtd5TLoaUIvTHFwg2u6bMrinz1FSZ-PA82JH_V00B3h-A3eTRjjDDjBTySd-kHOKzeS4V55T9Bbb2qQY</recordid><startdate>20160119</startdate><enddate>20160119</enddate><creator>Cano, Elena</creator><creator>Carmona, Rita</creator><creator>Ruiz-Villalba, Adrián</creator><creator>Rojas, Anabel</creator><creator>Chau, You-Ying</creator><creator>Wagner, Kay D.</creator><creator>Wagner, Nicole</creator><creator>Hastie, Nicholas D.</creator><creator>Muñoz-Chápuli, Ramón</creator><creator>Pérez-Pomares, José M.</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9686-473X</orcidid></search><sort><creationdate>20160119</creationdate><title>Extracardiac septum transversum/proepicardial endothelial cells pattern embryonic coronary arterio–venous connections</title><author>Cano, Elena ; 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However, the contribution of extracardiac cells to CoE is thought to be minor and nonsignificant for coronary formation. Using classic (Wt1Cre
) and previously undescribed (G2-Gata4Cre
) transgenic mouse models for the study of coronary vascular development, we show that extracardiac septum transversum/proepicardium (ST/PE)-derived endothelial cells are required for the formation of ventricular coronary arterio–venous vascular connections. Our results indicate that at least 20% of embryonic coronary arterial and capillary endothelial cells derive from the ST/PE compartment. Moreover, we show that conditional deletion of the ST/PE lineage-specific Wilms’ tumor suppressor gene (Wt1) in the ST/PE ofG2-Gata4Cre
mice and in the endothelium of Tie2Cre
mice disrupts embryonic coronary transmural patterning, leading to embryonic death. Taken together, our results demonstrate that ST/PE-derived endothelial cells contribute significantly to and are required for proper coronary vascular morphogenesis.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>26739565</pmid><doi>10.1073/pnas.1509834113</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-9686-473X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biological Sciences Biomarkers - metabolism Cell Lineage Cells Coronary Vessels - cytology Coronary Vessels - embryology Embryo, Mammalian - cytology Embryonic Development Endothelial Cells - cytology Enhancer Elements, Genetic - genetics Epithelial-Mesenchymal Transition GATA4 Transcription Factor - metabolism Gene Deletion Genes Genes, Reporter Green Fluorescent Proteins - metabolism Heart Heart Septum - cytology Integrases - metabolism Mice Models, Biological Myocytes, Cardiac - cytology Myocytes, Cardiac - metabolism Pericardium - cytology Phenotype Rodents Tumors WT1 Proteins - metabolism |
| title | Extracardiac septum transversum/proepicardial endothelial cells pattern embryonic coronary arterio–venous connections |
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