Role for topoisomerase 1 in transcription-associated mutagenesis in yeast

High levels of transcription in Saccharomyces cerevisiae are associated with increased genetic instability, which has been linked to DNA damage. Here, we describe a pGAL-CAN1 forward mutation assay for studying transcription-associated mutagenesis (TAM) in yeast. In a wild-type background with no al...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2011-01, Vol.108 (2), p.698-703
Hauptverfasser:	Lippert, Malcolm J., Kim, Nayun, Cho, Jang-Eun, Larson, Ryan P., Schoenly, Nathan E., O'Shea, Shannon H., Jinks-Robertson, Sue, Haber, James E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Alleles Base Sequence Biological Sciences CAN1 gene Conservation DNA DNA - genetics DNA Damage DNA Mutational Analysis DNA repair DNA Topoisomerases, Type I - genetics Enzymes Frameshift Mutation Fungal Proteins - genetics Gene Deletion Genes Genetic mutation hot spots Lesions Microsatellite repeats Models, Genetic Molecular Sequence Data Mutagenesis Mutagenesis, Site-Directed Mutation Open reading frames Reversion Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Signatures top1 gene Transcription Transcription, Genetic Yeast Yeasts
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