Ultra-sensitive, reusable, and superhydrophobic Ag/ZnO/Ag 3D hybrid surface enhanced Raman scattering substrate for hemoglobin detection
The small Raman scattering cross section of hemoglobin (Hb) molecules limits its application using a Raman spectroscopy based optical biosensor. Label-free surface enhanced Raman scattering (SERS) detection and degradation of Hb have been achieved using 3D reusable superhydrophobic SERS substrates b...
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description | The small Raman scattering cross section of hemoglobin (Hb) molecules limits its application using a Raman spectroscopy based optical biosensor. Label-free surface enhanced Raman scattering (SERS) detection and degradation of Hb have been achieved using 3D reusable superhydrophobic SERS substrates based on a Ag/ZnO/Ag hybrid structure. The fabrication process follows the decoration of thermally evaporated non-spherical like Ag nanoparticles on hydrothermally grown ZnO nanorods on a catalytic ultra-thin Ag film. From SERS point of view, these 3D SERS substrates exhibit four important characteristics such as a higher surface to volume ratio, surface plasmon resonance in the broad wavelength region of the visible spectrum, a strong electric field at the Ag–ZnO interface due to the formation of a Schottky barrier, and the superhydrophobic surface. The SERS substrates not only performed an outstanding Raman enhancement effect due to the above factors but also displayed multiple recyclabilities owing to their excellent self-cleaning property via a UV light assisted photocatalytic degradation process. The quantitative SERS analysis has been performed by a linear regression method and resulted in 10−13.42M and 10−7.24M limit of detection for Rhodamine 6G (Rh6G) and Hb molecules, respectively, with an enhancement factor of 6 × 1011. The effect of the 3D hybrid structure toward higher SERS activity has been compared with that of 2D SERS substrates, and the SERS mapping of Rh6G molecules proves good homogeneity of the 3D SERS substrates. These ultra-sensitive 3D SERS substrates with reusable capability open the possibility of their use toward biosensors for the early detection of diseases. |
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Label-free surface enhanced Raman scattering (SERS) detection and degradation of Hb have been achieved using 3D reusable superhydrophobic SERS substrates based on a Ag/ZnO/Ag hybrid structure. The fabrication process follows the decoration of thermally evaporated non-spherical like Ag nanoparticles on hydrothermally grown ZnO nanorods on a catalytic ultra-thin Ag film. From SERS point of view, these 3D SERS substrates exhibit four important characteristics such as a higher surface to volume ratio, surface plasmon resonance in the broad wavelength region of the visible spectrum, a strong electric field at the Ag–ZnO interface due to the formation of a Schottky barrier, and the superhydrophobic surface. The SERS substrates not only performed an outstanding Raman enhancement effect due to the above factors but also displayed multiple recyclabilities owing to their excellent self-cleaning property via a UV light assisted photocatalytic degradation process. The quantitative SERS analysis has been performed by a linear regression method and resulted in 10−13.42M and 10−7.24M limit of detection for Rhodamine 6G (Rh6G) and Hb molecules, respectively, with an enhancement factor of 6 × 1011. The effect of the 3D hybrid structure toward higher SERS activity has been compared with that of 2D SERS substrates, and the SERS mapping of Rh6G molecules proves good homogeneity of the 3D SERS substrates. These ultra-sensitive 3D SERS substrates with reusable capability open the possibility of their use toward biosensors for the early detection of diseases.</description><identifier>ISSN: 0021-8979</identifier><identifier>EISSN: 1089-7550</identifier><identifier>DOI: 10.1063/5.0007305</identifier><identifier>CODEN: JAPIAU</identifier><language>eng</language><publisher>Melville: American Institute of Physics</publisher><subject>Applied physics ; Biosensors ; Electric fields ; Free surfaces ; Hemoglobin ; Homogeneity ; Hybrid structures ; Hydrophobic surfaces ; Hydrophobicity ; Mapping ; Nanoparticles ; Nanorods ; Photodegradation ; Raman spectra ; Raman spectroscopy ; Regression analysis ; Rhodamine 6G ; Scattering cross sections ; Silver ; Substrates ; Ultraviolet radiation ; Visible spectrum ; Zinc oxide</subject><ispartof>Journal of applied physics, 2020-04, Vol.127 (16)</ispartof><rights>Author(s)</rights><rights>2020 Author(s). Published under license by AIP Publishing.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c327t-b6c8c128e7e5aa96b439bef033bb8d7645e713424993ce2f9c7209eb2799bf4a3</citedby><cites>FETCH-LOGICAL-c327t-b6c8c128e7e5aa96b439bef033bb8d7645e713424993ce2f9c7209eb2799bf4a3</cites><orcidid>0000-0003-4273-3248</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://pubs.aip.org/jap/article-lookup/doi/10.1063/5.0007305$$EHTML$$P50$$Gscitation$$H</linktohtml><link.rule.ids>314,777,781,791,4498,27905,27906,76133</link.rule.ids></links><search><creatorcontrib>Pal, Anil Kumar</creatorcontrib><creatorcontrib>Chandra, Goutam Kumar</creatorcontrib><creatorcontrib>Umapathy, Siva</creatorcontrib><creatorcontrib>Bharathi Mohan, D.</creatorcontrib><title>Ultra-sensitive, reusable, and superhydrophobic Ag/ZnO/Ag 3D hybrid surface enhanced Raman scattering substrate for hemoglobin detection</title><title>Journal of applied physics</title><description>The small Raman scattering cross section of hemoglobin (Hb) molecules limits its application using a Raman spectroscopy based optical biosensor. Label-free surface enhanced Raman scattering (SERS) detection and degradation of Hb have been achieved using 3D reusable superhydrophobic SERS substrates based on a Ag/ZnO/Ag hybrid structure. The fabrication process follows the decoration of thermally evaporated non-spherical like Ag nanoparticles on hydrothermally grown ZnO nanorods on a catalytic ultra-thin Ag film. From SERS point of view, these 3D SERS substrates exhibit four important characteristics such as a higher surface to volume ratio, surface plasmon resonance in the broad wavelength region of the visible spectrum, a strong electric field at the Ag–ZnO interface due to the formation of a Schottky barrier, and the superhydrophobic surface. The SERS substrates not only performed an outstanding Raman enhancement effect due to the above factors but also displayed multiple recyclabilities owing to their excellent self-cleaning property via a UV light assisted photocatalytic degradation process. The quantitative SERS analysis has been performed by a linear regression method and resulted in 10−13.42M and 10−7.24M limit of detection for Rhodamine 6G (Rh6G) and Hb molecules, respectively, with an enhancement factor of 6 × 1011. The effect of the 3D hybrid structure toward higher SERS activity has been compared with that of 2D SERS substrates, and the SERS mapping of Rh6G molecules proves good homogeneity of the 3D SERS substrates. These ultra-sensitive 3D SERS substrates with reusable capability open the possibility of their use toward biosensors for the early detection of diseases.</description><subject>Applied physics</subject><subject>Biosensors</subject><subject>Electric fields</subject><subject>Free surfaces</subject><subject>Hemoglobin</subject><subject>Homogeneity</subject><subject>Hybrid structures</subject><subject>Hydrophobic surfaces</subject><subject>Hydrophobicity</subject><subject>Mapping</subject><subject>Nanoparticles</subject><subject>Nanorods</subject><subject>Photodegradation</subject><subject>Raman spectra</subject><subject>Raman spectroscopy</subject><subject>Regression analysis</subject><subject>Rhodamine 6G</subject><subject>Scattering cross sections</subject><subject>Silver</subject><subject>Substrates</subject><subject>Ultraviolet radiation</subject><subject>Visible spectrum</subject><subject>Zinc oxide</subject><issn>0021-8979</issn><issn>1089-7550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp90M9LwzAUB_AgCs7pwf8g4EmxW9K0TXMc8ycMBuIuXkqSvq4dW1KTdLD_wD_bjg09CJ7eO3z4Pt4XoWtKRpRkbJyOCCGckfQEDSjJRcTTlJyiASExjXLBxTm68H5FCKU5EwP0tVgHJyMPxjeh2cI9dtB5qdb9Jk2JfdeCq3els21tVaPxZDn-MPPxZInZA653yjV75CqpAYOppdFQ4je5kQZ7LUMA15hlL5Tv7wTAlXW4ho1drvs4g0sIoENjzSU6q-Taw9VxDtHi6fF9-hLN5s-v08ks0izmIVKZzjWNc-CQSikylTChoCKMKZWXPEtS4JQlcSIE0xBXQvOYCFAxF0JViWRDdHPIbZ397MCHYmU7Z_qTRcxESrKk76VXtwelnfXeQVW0rtlItysoKfZFF2lxLLq3dwfrdRPk_pcfvLXuFxZtWf2H_yZ_AwB8ja0</recordid><startdate>20200430</startdate><enddate>20200430</enddate><creator>Pal, Anil Kumar</creator><creator>Chandra, Goutam Kumar</creator><creator>Umapathy, Siva</creator><creator>Bharathi Mohan, D.</creator><general>American Institute of Physics</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>H8D</scope><scope>L7M</scope><orcidid>https://orcid.org/0000-0003-4273-3248</orcidid></search><sort><creationdate>20200430</creationdate><title>Ultra-sensitive, reusable, and superhydrophobic Ag/ZnO/Ag 3D hybrid surface enhanced Raman scattering substrate for hemoglobin detection</title><author>Pal, Anil Kumar ; Chandra, Goutam Kumar ; Umapathy, Siva ; Bharathi Mohan, D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c327t-b6c8c128e7e5aa96b439bef033bb8d7645e713424993ce2f9c7209eb2799bf4a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Applied physics</topic><topic>Biosensors</topic><topic>Electric fields</topic><topic>Free surfaces</topic><topic>Hemoglobin</topic><topic>Homogeneity</topic><topic>Hybrid structures</topic><topic>Hydrophobic surfaces</topic><topic>Hydrophobicity</topic><topic>Mapping</topic><topic>Nanoparticles</topic><topic>Nanorods</topic><topic>Photodegradation</topic><topic>Raman spectra</topic><topic>Raman spectroscopy</topic><topic>Regression analysis</topic><topic>Rhodamine 6G</topic><topic>Scattering cross sections</topic><topic>Silver</topic><topic>Substrates</topic><topic>Ultraviolet radiation</topic><topic>Visible spectrum</topic><topic>Zinc oxide</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pal, Anil Kumar</creatorcontrib><creatorcontrib>Chandra, Goutam Kumar</creatorcontrib><creatorcontrib>Umapathy, Siva</creatorcontrib><creatorcontrib>Bharathi Mohan, D.</creatorcontrib><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Aerospace Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Journal of applied physics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pal, Anil Kumar</au><au>Chandra, Goutam Kumar</au><au>Umapathy, Siva</au><au>Bharathi Mohan, D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultra-sensitive, reusable, and superhydrophobic Ag/ZnO/Ag 3D hybrid surface enhanced Raman scattering substrate for hemoglobin detection</atitle><jtitle>Journal of applied physics</jtitle><date>2020-04-30</date><risdate>2020</risdate><volume>127</volume><issue>16</issue><issn>0021-8979</issn><eissn>1089-7550</eissn><coden>JAPIAU</coden><abstract>The small Raman scattering cross section of hemoglobin (Hb) molecules limits its application using a Raman spectroscopy based optical biosensor. Label-free surface enhanced Raman scattering (SERS) detection and degradation of Hb have been achieved using 3D reusable superhydrophobic SERS substrates based on a Ag/ZnO/Ag hybrid structure. The fabrication process follows the decoration of thermally evaporated non-spherical like Ag nanoparticles on hydrothermally grown ZnO nanorods on a catalytic ultra-thin Ag film. From SERS point of view, these 3D SERS substrates exhibit four important characteristics such as a higher surface to volume ratio, surface plasmon resonance in the broad wavelength region of the visible spectrum, a strong electric field at the Ag–ZnO interface due to the formation of a Schottky barrier, and the superhydrophobic surface. The SERS substrates not only performed an outstanding Raman enhancement effect due to the above factors but also displayed multiple recyclabilities owing to their excellent self-cleaning property via a UV light assisted photocatalytic degradation process. The quantitative SERS analysis has been performed by a linear regression method and resulted in 10−13.42M and 10−7.24M limit of detection for Rhodamine 6G (Rh6G) and Hb molecules, respectively, with an enhancement factor of 6 × 1011. The effect of the 3D hybrid structure toward higher SERS activity has been compared with that of 2D SERS substrates, and the SERS mapping of Rh6G molecules proves good homogeneity of the 3D SERS substrates. These ultra-sensitive 3D SERS substrates with reusable capability open the possibility of their use toward biosensors for the early detection of diseases.</abstract><cop>Melville</cop><pub>American Institute of Physics</pub><doi>10.1063/5.0007305</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-4273-3248</orcidid></addata></record> |
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subjects | Applied physics Biosensors Electric fields Free surfaces Hemoglobin Homogeneity Hybrid structures Hydrophobic surfaces Hydrophobicity Mapping Nanoparticles Nanorods Photodegradation Raman spectra Raman spectroscopy Regression analysis Rhodamine 6G Scattering cross sections Silver Substrates Ultraviolet radiation Visible spectrum Zinc oxide |
title | Ultra-sensitive, reusable, and superhydrophobic Ag/ZnO/Ag 3D hybrid surface enhanced Raman scattering substrate for hemoglobin detection |
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