Live cells assessment of opto-poration by a single femtosecond temporal Airy laser pulse
We report on the first study of live cell opto-poration by single temporally shaped femtosecond laser pulses. Based on an ad hoc developed cell staining protocol, we demonstrate the influence of the pulse temporal profile on the efficiency of poration and on cell viability at four hours comparing th...
Gespeichert in:
| Veröffentlicht in: | AIP advances 2018-12, Vol.8 (12), p.125105-125105-10 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 125105-10 |
|---|---|
| container_issue | 12 |
| container_start_page | 125105 |
| container_title | AIP advances |
| container_volume | 8 |
| creator | Campargue, G. Zielinski, B. Courvoisier, S. Sarpe, C. Winkler, T. Sentfleben, A. Bonacina, L. Baumert, T. Wolf, J. P. |
| description | We report on the first study of live cell opto-poration by single temporally shaped
femtosecond laser pulses. Based on an ad hoc developed cell staining
protocol, we demonstrate the influence of the pulse temporal profile on the efficiency of
poration and on cell viability at four hours comparing the results obtained for four
different temporal pulse shapes: positive and negative temporal Airy, positively chirped,
and 30 fs bandwidth limited pulses. Each pulse has been tested on a thousand cells. The
most suitable pulses for opto-poration are the positive Temporal Airy Pulses (TAP+),
likely because they enhance avalanche ionization compared to bandwidth-limited shorter
pulses. We discuss the results in the context of previous studies, highlighting the
differences between single and multi-pulse opto-poration strategies. |
| doi_str_mv | 10.1063/1.5049678 |
| format | Article |
| fullrecord | <record><control><sourceid>scitation_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1063_1_5049678</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_c115277af4a44f82bceb3fe27ca4b759</doaj_id><sourcerecordid>adv</sourcerecordid><originalsourceid>FETCH-LOGICAL-c365t-83203f1cb04cf1bdfb40fc225c8b880e8b9e70424a9e2c2b84437263f3d4239f3</originalsourceid><addsrcrecordid>eNp9kEtLxDAUhYMoOIyz8B9kq1DNq226HAYfAwNuFNyFJL0ZMrRNSaIw_97OA3Hl3ZzL4eNbHIRuKXmgpOKP9KEkoqlqeYFmjJay4IxVl3_-a7RIaUemEw0lUszQ58Z_A7bQdQnrlCClHoaMg8NhzKEYQ9TZhwGbPdY4-WHbAXbQ55DAhqHFGfoD0-Glj3vc6QQRj19dght05fSUi3PO0cfz0_vqtdi8vaxXy01heVXmQnJGuKPWEGEdNa0zgjjLWGmlkZKANA3URDChG2CWGSkEr1nFHW8F443jc7Q-edugd2qMvtdxr4L26liEuFU6Zm87UJbSktW1dkIL4SQzFgx3wGqrhanLZnLdnVw2hpQiuF8fJeqwsKLqvPDE3p_YZH0-bvQP_AOslHsu</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Live cells assessment of opto-poration by a single femtosecond temporal Airy laser pulse</title><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Campargue, G. ; Zielinski, B. ; Courvoisier, S. ; Sarpe, C. ; Winkler, T. ; Sentfleben, A. ; Bonacina, L. ; Baumert, T. ; Wolf, J. P.</creator><creatorcontrib>Campargue, G. ; Zielinski, B. ; Courvoisier, S. ; Sarpe, C. ; Winkler, T. ; Sentfleben, A. ; Bonacina, L. ; Baumert, T. ; Wolf, J. P.</creatorcontrib><description>We report on the first study of live cell opto-poration by single temporally shaped
femtosecond laser pulses. Based on an ad hoc developed cell staining
protocol, we demonstrate the influence of the pulse temporal profile on the efficiency of
poration and on cell viability at four hours comparing the results obtained for four
different temporal pulse shapes: positive and negative temporal Airy, positively chirped,
and 30 fs bandwidth limited pulses. Each pulse has been tested on a thousand cells. The
most suitable pulses for opto-poration are the positive Temporal Airy Pulses (TAP+),
likely because they enhance avalanche ionization compared to bandwidth-limited shorter
pulses. We discuss the results in the context of previous studies, highlighting the
differences between single and multi-pulse opto-poration strategies.</description><identifier>ISSN: 2158-3226</identifier><identifier>EISSN: 2158-3226</identifier><identifier>DOI: 10.1063/1.5049678</identifier><identifier>CODEN: AAIDBI</identifier><language>eng</language><publisher>AIP Publishing LLC</publisher><ispartof>AIP advances, 2018-12, Vol.8 (12), p.125105-125105-10</ispartof><rights>Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-83203f1cb04cf1bdfb40fc225c8b880e8b9e70424a9e2c2b84437263f3d4239f3</citedby><cites>FETCH-LOGICAL-c365t-83203f1cb04cf1bdfb40fc225c8b880e8b9e70424a9e2c2b84437263f3d4239f3</cites><orcidid>0000-0003-0476-4473 ; 0000-0003-3729-958X ; 0000-0002-3097-0230</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,2102,27924,27925</link.rule.ids></links><search><creatorcontrib>Campargue, G.</creatorcontrib><creatorcontrib>Zielinski, B.</creatorcontrib><creatorcontrib>Courvoisier, S.</creatorcontrib><creatorcontrib>Sarpe, C.</creatorcontrib><creatorcontrib>Winkler, T.</creatorcontrib><creatorcontrib>Sentfleben, A.</creatorcontrib><creatorcontrib>Bonacina, L.</creatorcontrib><creatorcontrib>Baumert, T.</creatorcontrib><creatorcontrib>Wolf, J. P.</creatorcontrib><title>Live cells assessment of opto-poration by a single femtosecond temporal Airy laser pulse</title><title>AIP advances</title><description>We report on the first study of live cell opto-poration by single temporally shaped
femtosecond laser pulses. Based on an ad hoc developed cell staining
protocol, we demonstrate the influence of the pulse temporal profile on the efficiency of
poration and on cell viability at four hours comparing the results obtained for four
different temporal pulse shapes: positive and negative temporal Airy, positively chirped,
and 30 fs bandwidth limited pulses. Each pulse has been tested on a thousand cells. The
most suitable pulses for opto-poration are the positive Temporal Airy Pulses (TAP+),
likely because they enhance avalanche ionization compared to bandwidth-limited shorter
pulses. We discuss the results in the context of previous studies, highlighting the
differences between single and multi-pulse opto-poration strategies.</description><issn>2158-3226</issn><issn>2158-3226</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNp9kEtLxDAUhYMoOIyz8B9kq1DNq226HAYfAwNuFNyFJL0ZMrRNSaIw_97OA3Hl3ZzL4eNbHIRuKXmgpOKP9KEkoqlqeYFmjJay4IxVl3_-a7RIaUemEw0lUszQ58Z_A7bQdQnrlCClHoaMg8NhzKEYQ9TZhwGbPdY4-WHbAXbQ55DAhqHFGfoD0-Glj3vc6QQRj19dght05fSUi3PO0cfz0_vqtdi8vaxXy01heVXmQnJGuKPWEGEdNa0zgjjLWGmlkZKANA3URDChG2CWGSkEr1nFHW8F443jc7Q-edugd2qMvtdxr4L26liEuFU6Zm87UJbSktW1dkIL4SQzFgx3wGqrhanLZnLdnVw2hpQiuF8fJeqwsKLqvPDE3p_YZH0-bvQP_AOslHsu</recordid><startdate>201812</startdate><enddate>201812</enddate><creator>Campargue, G.</creator><creator>Zielinski, B.</creator><creator>Courvoisier, S.</creator><creator>Sarpe, C.</creator><creator>Winkler, T.</creator><creator>Sentfleben, A.</creator><creator>Bonacina, L.</creator><creator>Baumert, T.</creator><creator>Wolf, J. P.</creator><general>AIP Publishing LLC</general><scope>AJDQP</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-0476-4473</orcidid><orcidid>https://orcid.org/0000-0003-3729-958X</orcidid><orcidid>https://orcid.org/0000-0002-3097-0230</orcidid></search><sort><creationdate>201812</creationdate><title>Live cells assessment of opto-poration by a single femtosecond temporal Airy laser pulse</title><author>Campargue, G. ; Zielinski, B. ; Courvoisier, S. ; Sarpe, C. ; Winkler, T. ; Sentfleben, A. ; Bonacina, L. ; Baumert, T. ; Wolf, J. P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-83203f1cb04cf1bdfb40fc225c8b880e8b9e70424a9e2c2b84437263f3d4239f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Campargue, G.</creatorcontrib><creatorcontrib>Zielinski, B.</creatorcontrib><creatorcontrib>Courvoisier, S.</creatorcontrib><creatorcontrib>Sarpe, C.</creatorcontrib><creatorcontrib>Winkler, T.</creatorcontrib><creatorcontrib>Sentfleben, A.</creatorcontrib><creatorcontrib>Bonacina, L.</creatorcontrib><creatorcontrib>Baumert, T.</creatorcontrib><creatorcontrib>Wolf, J. P.</creatorcontrib><collection>AIP Open Access Journals</collection><collection>CrossRef</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>AIP advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Campargue, G.</au><au>Zielinski, B.</au><au>Courvoisier, S.</au><au>Sarpe, C.</au><au>Winkler, T.</au><au>Sentfleben, A.</au><au>Bonacina, L.</au><au>Baumert, T.</au><au>Wolf, J. P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Live cells assessment of opto-poration by a single femtosecond temporal Airy laser pulse</atitle><jtitle>AIP advances</jtitle><date>2018-12</date><risdate>2018</risdate><volume>8</volume><issue>12</issue><spage>125105</spage><epage>125105-10</epage><pages>125105-125105-10</pages><issn>2158-3226</issn><eissn>2158-3226</eissn><coden>AAIDBI</coden><abstract>We report on the first study of live cell opto-poration by single temporally shaped
femtosecond laser pulses. Based on an ad hoc developed cell staining
protocol, we demonstrate the influence of the pulse temporal profile on the efficiency of
poration and on cell viability at four hours comparing the results obtained for four
different temporal pulse shapes: positive and negative temporal Airy, positively chirped,
and 30 fs bandwidth limited pulses. Each pulse has been tested on a thousand cells. The
most suitable pulses for opto-poration are the positive Temporal Airy Pulses (TAP+),
likely because they enhance avalanche ionization compared to bandwidth-limited shorter
pulses. We discuss the results in the context of previous studies, highlighting the
differences between single and multi-pulse opto-poration strategies.</abstract><pub>AIP Publishing LLC</pub><doi>10.1063/1.5049678</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-0476-4473</orcidid><orcidid>https://orcid.org/0000-0003-3729-958X</orcidid><orcidid>https://orcid.org/0000-0002-3097-0230</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2158-3226 |
| ispartof | AIP advances, 2018-12, Vol.8 (12), p.125105-125105-10 |
| issn | 2158-3226 2158-3226 |
| language | eng |
| recordid | cdi_crossref_primary_10_1063_1_5049678 |
| source | DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| title | Live cells assessment of opto-poration by a single femtosecond temporal Airy laser pulse |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T21%3A18%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-scitation_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Live%20cells%20assessment%20of%20opto-poration%20by%20a%20single%20femtosecond%20temporal%20Airy%20laser%20pulse&rft.jtitle=AIP%20advances&rft.au=Campargue,%20G.&rft.date=2018-12&rft.volume=8&rft.issue=12&rft.spage=125105&rft.epage=125105-10&rft.pages=125105-125105-10&rft.issn=2158-3226&rft.eissn=2158-3226&rft.coden=AAIDBI&rft_id=info:doi/10.1063/1.5049678&rft_dat=%3Cscitation_cross%3Eadv%3C/scitation_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_doaj_id=oai_doaj_org_article_c115277af4a44f82bceb3fe27ca4b759&rfr_iscdi=true |