Aptamer strategy for ATP detection on nanocrystalline diamond functionalized by a nitrogen and hydrogen radical beam system
Here, we report a novel method for micropatterning oligonucleotides on the diamond surface via forming amine groups on the diamond surface by nitrogen/hydrogen radical treatment. The covalent bonding of the supporting oligonucleotide and characterization of an immobilized hybridized oligonucleotide...
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| Veröffentlicht in: | Journal of applied physics 2017-01, Vol.121 (4) |
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| container_title | Journal of applied physics |
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| creator | Suaebah, E. Seshimo, Y. Shibata, M. Kono, S. Hasegawa, M. Kawarada, H. |
| description | Here, we report a novel method for micropatterning oligonucleotides on the diamond surface via forming amine groups on the diamond surface by nitrogen/hydrogen radical treatment. The covalent bonding of the supporting oligonucleotide and characterization of an immobilized hybridized oligonucleotide with Cy5 modification were investigated by fluorescence microscopy. To investigate the effectiveness of nitrogen/hydrogen radical treatment for amine termination, two types of radical treatment were used: hydrogen/nitrogen radical treatment and pure nitrogen radical treatment. From the results, hydrogen/nitrogen radical treatment produces amine (NH2) termination on the diamond surface. The effect of amine termination was investigated by immobilization of single-stranded DNA via amide bonding between surface NH2 groups and COOH groups terminating the DNA. The immobilized single-stranded DNA (supporting DNA), which has a complementary relationship with the adenosine triphosphate (ATP) aptamer (DNA), hybridizes with the aptamer with attached fluorescence dye. When ATP molecules approach the double-stranded DNA, the aptamer forms a close relationship with the supporting DNA and combines with ATP. ATP detection was effectively carried out by reduction of fluorescence. |
| doi_str_mv | 10.1063/1.4974984 |
| format | Article |
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The covalent bonding of the supporting oligonucleotide and characterization of an immobilized hybridized oligonucleotide with Cy5 modification were investigated by fluorescence microscopy. To investigate the effectiveness of nitrogen/hydrogen radical treatment for amine termination, two types of radical treatment were used: hydrogen/nitrogen radical treatment and pure nitrogen radical treatment. From the results, hydrogen/nitrogen radical treatment produces amine (NH2) termination on the diamond surface. The effect of amine termination was investigated by immobilization of single-stranded DNA via amide bonding between surface NH2 groups and COOH groups terminating the DNA. The immobilized single-stranded DNA (supporting DNA), which has a complementary relationship with the adenosine triphosphate (ATP) aptamer (DNA), hybridizes with the aptamer with attached fluorescence dye. When ATP molecules approach the double-stranded DNA, the aptamer forms a close relationship with the supporting DNA and combines with ATP. ATP detection was effectively carried out by reduction of fluorescence.</description><identifier>ISSN: 0021-8979</identifier><identifier>EISSN: 1089-7550</identifier><identifier>DOI: 10.1063/1.4974984</identifier><identifier>CODEN: JAPIAU</identifier><language>eng</language><publisher>Melville: American Institute of Physics</publisher><subject>Adenosine triphosphate ; Applied physics ; Chief executive officers ; Corporate reorganization ; Deoxyribonucleic acid ; Diamonds ; DNA ; Fluorescence ; Hydrogen ; Micropatterning ; Nitrogen ; Oligonucleotides ; Settlements & damages</subject><ispartof>Journal of applied physics, 2017-01, Vol.121 (4)</ispartof><rights>Author(s)</rights><rights>2017 Author(s). 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The covalent bonding of the supporting oligonucleotide and characterization of an immobilized hybridized oligonucleotide with Cy5 modification were investigated by fluorescence microscopy. To investigate the effectiveness of nitrogen/hydrogen radical treatment for amine termination, two types of radical treatment were used: hydrogen/nitrogen radical treatment and pure nitrogen radical treatment. From the results, hydrogen/nitrogen radical treatment produces amine (NH2) termination on the diamond surface. The effect of amine termination was investigated by immobilization of single-stranded DNA via amide bonding between surface NH2 groups and COOH groups terminating the DNA. The immobilized single-stranded DNA (supporting DNA), which has a complementary relationship with the adenosine triphosphate (ATP) aptamer (DNA), hybridizes with the aptamer with attached fluorescence dye. When ATP molecules approach the double-stranded DNA, the aptamer forms a close relationship with the supporting DNA and combines with ATP. ATP detection was effectively carried out by reduction of fluorescence.</description><subject>Adenosine triphosphate</subject><subject>Applied physics</subject><subject>Chief executive officers</subject><subject>Corporate reorganization</subject><subject>Deoxyribonucleic acid</subject><subject>Diamonds</subject><subject>DNA</subject><subject>Fluorescence</subject><subject>Hydrogen</subject><subject>Micropatterning</subject><subject>Nitrogen</subject><subject>Oligonucleotides</subject><subject>Settlements & damages</subject><issn>0021-8979</issn><issn>1089-7550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNqd0MtKxDAUBuAgCo6XhW8QcKXQMektyXIYvMGALsZ1OG3SMUPb1CQjVF_ejB1wLxw4HPj4OfwIXVEyp6TM7ug8FywXPD9CM0q4SFhRkGM0IySlCRdMnKIz77eEUMozMUPfiyFApx32wUHQmxE31uHF-hUrHXQdjO1xnB56W7vRB2hb02usDHS2V7jZ9b8GWvOlFa5GDLg3wdmN7jFE8D6q6XCgTA0trjR02Mck3V2gkwZary8P-xy9Pdyvl0_J6uXxeblYJXUmshC_LijUQFTZCC1SlhMGWhOaKih5leZQllVVcMGzhjHFONMlIQUrgAtSFWWTnaPrKXdw9mOnfZBbu3PxZy9Tmual4FFHdTOp2lnvnW7k4EwHbpSUyH23kspDt9HeTtbXJsC-gP_hT-v-oBxUk_0ATZGJVA</recordid><startdate>20170128</startdate><enddate>20170128</enddate><creator>Suaebah, E.</creator><creator>Seshimo, Y.</creator><creator>Shibata, M.</creator><creator>Kono, S.</creator><creator>Hasegawa, M.</creator><creator>Kawarada, H.</creator><general>American Institute of Physics</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>H8D</scope><scope>L7M</scope></search><sort><creationdate>20170128</creationdate><title>Aptamer strategy for ATP detection on nanocrystalline diamond functionalized by a nitrogen and hydrogen radical beam system</title><author>Suaebah, E. ; Seshimo, Y. ; Shibata, M. ; Kono, S. ; Hasegawa, M. ; Kawarada, H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c393t-8951aca0d6f9e927407aee012da68b24a66bb58983f77d787e600575a890b56f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adenosine triphosphate</topic><topic>Applied physics</topic><topic>Chief executive officers</topic><topic>Corporate reorganization</topic><topic>Deoxyribonucleic acid</topic><topic>Diamonds</topic><topic>DNA</topic><topic>Fluorescence</topic><topic>Hydrogen</topic><topic>Micropatterning</topic><topic>Nitrogen</topic><topic>Oligonucleotides</topic><topic>Settlements & damages</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suaebah, E.</creatorcontrib><creatorcontrib>Seshimo, Y.</creatorcontrib><creatorcontrib>Shibata, M.</creatorcontrib><creatorcontrib>Kono, S.</creatorcontrib><creatorcontrib>Hasegawa, M.</creatorcontrib><creatorcontrib>Kawarada, H.</creatorcontrib><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Aerospace Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Journal of applied physics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suaebah, E.</au><au>Seshimo, Y.</au><au>Shibata, M.</au><au>Kono, S.</au><au>Hasegawa, M.</au><au>Kawarada, H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aptamer strategy for ATP detection on nanocrystalline diamond functionalized by a nitrogen and hydrogen radical beam system</atitle><jtitle>Journal of applied physics</jtitle><date>2017-01-28</date><risdate>2017</risdate><volume>121</volume><issue>4</issue><issn>0021-8979</issn><eissn>1089-7550</eissn><coden>JAPIAU</coden><abstract>Here, we report a novel method for micropatterning oligonucleotides on the diamond surface via forming amine groups on the diamond surface by nitrogen/hydrogen radical treatment. The covalent bonding of the supporting oligonucleotide and characterization of an immobilized hybridized oligonucleotide with Cy5 modification were investigated by fluorescence microscopy. To investigate the effectiveness of nitrogen/hydrogen radical treatment for amine termination, two types of radical treatment were used: hydrogen/nitrogen radical treatment and pure nitrogen radical treatment. From the results, hydrogen/nitrogen radical treatment produces amine (NH2) termination on the diamond surface. The effect of amine termination was investigated by immobilization of single-stranded DNA via amide bonding between surface NH2 groups and COOH groups terminating the DNA. The immobilized single-stranded DNA (supporting DNA), which has a complementary relationship with the adenosine triphosphate (ATP) aptamer (DNA), hybridizes with the aptamer with attached fluorescence dye. When ATP molecules approach the double-stranded DNA, the aptamer forms a close relationship with the supporting DNA and combines with ATP. ATP detection was effectively carried out by reduction of fluorescence.</abstract><cop>Melville</cop><pub>American Institute of Physics</pub><doi>10.1063/1.4974984</doi><tpages>6</tpages></addata></record> |
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| source | AIP Journals Complete; Alma/SFX Local Collection |
| subjects | Adenosine triphosphate Applied physics Chief executive officers Corporate reorganization Deoxyribonucleic acid Diamonds DNA Fluorescence Hydrogen Micropatterning Nitrogen Oligonucleotides Settlements & damages |
| title | Aptamer strategy for ATP detection on nanocrystalline diamond functionalized by a nitrogen and hydrogen radical beam system |
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