Microfluidic device for trapping and monitoring three dimensional multicell spheroids using electrical impedance spectroscopy
In this paper, we report the design, fabrication, and testing of a lab-on-a-chip based microfluidic device for application of trapping and measuring the dielectric properties of microtumors over time using electrical impedance spectroscopy (EIS). Microelectromechanical system (MEMS) techniques were...
Gespeichert in:
Veröffentlicht in: | Biomicrofluidics 2013-05, Vol.7 (3), p.34108-34108 |
---|---|
Hauptverfasser: | , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 34108 |
---|---|
container_issue | 3 |
container_start_page | 34108 |
container_title | Biomicrofluidics |
container_volume | 7 |
creator | Luongo, Kevin Holton, Angela Kaushik, Ajeet Spence, Paige Ng, Beng Deschenes, Robert Sundaram, Shankar Bhansali, Shekhar |
description | In this paper, we report the design, fabrication, and testing of a lab-on-a-chip based microfluidic device for application of trapping and measuring the dielectric properties of microtumors over time using electrical impedance spectroscopy (EIS). Microelectromechanical system (MEMS) techniques were used to embed opposing electrodes onto the top and bottom surfaces of a microfluidic channel fabricated using Pyrex substrate, chrome gold, SU-8, and polydimethylsiloxane. Differing concentrations of cell culture medium, differing sized polystyrene beads, and MCF-7 microtumor spheroids were used to validate the designs ability to detect background conductivity changes and dielectric particle diameter changes between electrodes. The observed changes in cell medium concentrations demonstrated a linear relation to extracted solution resistance (Rs), while polystyrene beads and multicell spheroids induced changes in magnitude consistent with diameter increase. This design permits optical correlation between electrical measurements and EIS spectra. |
doi_str_mv | 10.1063/1.4809590 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1063_1_4809590</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1490703916</sourcerecordid><originalsourceid>FETCH-LOGICAL-c511t-ab15ae105e671d68b2af5814b0f2009c8c9df65f26a339562865f181f76bac1e3</originalsourceid><addsrcrecordid>eNp9kUuPFCEUhYnROOPowj9gWKpJj1yqoGFjMpn4Ssa40TWheExjqgChqpNZ-N-l0m07xsQVXPg4l3MPQs-BXALh3Ru47AWRTJIH6BxkRzdAmHh4b3-GntT6nRAGW0ofozPa96QnVJyjn5-DKcmPS7DBYOv2wTjsU8Fz0TmHeIt1tHhKMcyprOW8K85hGyYXa0hRj3haxrm9Gkdc886VFGzFS11ZNzozl2AaFKbsrI5NvOb1MFWT8t1T9Mjrsbpnx_UCfXv_7uv1x83Nlw-frq9uNoYBzBs9ANOuGXF8C5aLgWrPBPQD8ZQQaYSR1nPmKdddJxmnohUgwG_5oA247gK9PejmZZicNS42e6PKJUy63Kmkg_r7Joaduk171XEhBWVN4OVRoKQfi6uzmkJdPevo0lIV9JJsSSeBN_TVAW1zrbU4f2oDRK1xKVDHuBr74v6_TuTvfBrw-gBUE2Y9t4GfmH0qf5RUtv5_8L-tfwHm87A2</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1490703916</pqid></control><display><type>article</type><title>Microfluidic device for trapping and monitoring three dimensional multicell spheroids using electrical impedance spectroscopy</title><source>AIP Journals Complete</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Luongo, Kevin ; Holton, Angela ; Kaushik, Ajeet ; Spence, Paige ; Ng, Beng ; Deschenes, Robert ; Sundaram, Shankar ; Bhansali, Shekhar</creator><creatorcontrib>Luongo, Kevin ; Holton, Angela ; Kaushik, Ajeet ; Spence, Paige ; Ng, Beng ; Deschenes, Robert ; Sundaram, Shankar ; Bhansali, Shekhar</creatorcontrib><description>In this paper, we report the design, fabrication, and testing of a lab-on-a-chip based microfluidic device for application of trapping and measuring the dielectric properties of microtumors over time using electrical impedance spectroscopy (EIS). Microelectromechanical system (MEMS) techniques were used to embed opposing electrodes onto the top and bottom surfaces of a microfluidic channel fabricated using Pyrex substrate, chrome gold, SU-8, and polydimethylsiloxane. Differing concentrations of cell culture medium, differing sized polystyrene beads, and MCF-7 microtumor spheroids were used to validate the designs ability to detect background conductivity changes and dielectric particle diameter changes between electrodes. The observed changes in cell medium concentrations demonstrated a linear relation to extracted solution resistance (Rs), while polystyrene beads and multicell spheroids induced changes in magnitude consistent with diameter increase. This design permits optical correlation between electrical measurements and EIS spectra.</description><identifier>ISSN: 1932-1058</identifier><identifier>EISSN: 1932-1058</identifier><identifier>DOI: 10.1063/1.4809590</identifier><identifier>PMID: 24404028</identifier><identifier>CODEN: BIOMGB</identifier><language>eng</language><publisher>United States: AIP Publishing LLC</publisher><subject>Regular</subject><ispartof>Biomicrofluidics, 2013-05, Vol.7 (3), p.34108-34108</ispartof><rights>AIP Publishing LLC</rights><rights>Copyright © 2013 AIP Publishing LLC 2013 AIP Publishing LLC</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c511t-ab15ae105e671d68b2af5814b0f2009c8c9df65f26a339562865f181f76bac1e3</citedby><cites>FETCH-LOGICAL-c511t-ab15ae105e671d68b2af5814b0f2009c8c9df65f26a339562865f181f76bac1e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689825/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://pubs.aip.org/bmf/article-lookup/doi/10.1063/1.4809590$$EHTML$$P50$$Gscitation$$H</linktohtml><link.rule.ids>230,314,727,780,784,794,885,4512,27924,27925,53791,53793,76384</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24404028$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Luongo, Kevin</creatorcontrib><creatorcontrib>Holton, Angela</creatorcontrib><creatorcontrib>Kaushik, Ajeet</creatorcontrib><creatorcontrib>Spence, Paige</creatorcontrib><creatorcontrib>Ng, Beng</creatorcontrib><creatorcontrib>Deschenes, Robert</creatorcontrib><creatorcontrib>Sundaram, Shankar</creatorcontrib><creatorcontrib>Bhansali, Shekhar</creatorcontrib><title>Microfluidic device for trapping and monitoring three dimensional multicell spheroids using electrical impedance spectroscopy</title><title>Biomicrofluidics</title><addtitle>Biomicrofluidics</addtitle><description>In this paper, we report the design, fabrication, and testing of a lab-on-a-chip based microfluidic device for application of trapping and measuring the dielectric properties of microtumors over time using electrical impedance spectroscopy (EIS). Microelectromechanical system (MEMS) techniques were used to embed opposing electrodes onto the top and bottom surfaces of a microfluidic channel fabricated using Pyrex substrate, chrome gold, SU-8, and polydimethylsiloxane. Differing concentrations of cell culture medium, differing sized polystyrene beads, and MCF-7 microtumor spheroids were used to validate the designs ability to detect background conductivity changes and dielectric particle diameter changes between electrodes. The observed changes in cell medium concentrations demonstrated a linear relation to extracted solution resistance (Rs), while polystyrene beads and multicell spheroids induced changes in magnitude consistent with diameter increase. This design permits optical correlation between electrical measurements and EIS spectra.</description><subject>Regular</subject><issn>1932-1058</issn><issn>1932-1058</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNp9kUuPFCEUhYnROOPowj9gWKpJj1yqoGFjMpn4Ssa40TWheExjqgChqpNZ-N-l0m07xsQVXPg4l3MPQs-BXALh3Ru47AWRTJIH6BxkRzdAmHh4b3-GntT6nRAGW0ofozPa96QnVJyjn5-DKcmPS7DBYOv2wTjsU8Fz0TmHeIt1tHhKMcyprOW8K85hGyYXa0hRj3haxrm9Gkdc886VFGzFS11ZNzozl2AaFKbsrI5NvOb1MFWT8t1T9Mjrsbpnx_UCfXv_7uv1x83Nlw-frq9uNoYBzBs9ANOuGXF8C5aLgWrPBPQD8ZQQaYSR1nPmKdddJxmnohUgwG_5oA247gK9PejmZZicNS42e6PKJUy63Kmkg_r7Joaduk171XEhBWVN4OVRoKQfi6uzmkJdPevo0lIV9JJsSSeBN_TVAW1zrbU4f2oDRK1xKVDHuBr74v6_TuTvfBrw-gBUE2Y9t4GfmH0qf5RUtv5_8L-tfwHm87A2</recordid><startdate>20130501</startdate><enddate>20130501</enddate><creator>Luongo, Kevin</creator><creator>Holton, Angela</creator><creator>Kaushik, Ajeet</creator><creator>Spence, Paige</creator><creator>Ng, Beng</creator><creator>Deschenes, Robert</creator><creator>Sundaram, Shankar</creator><creator>Bhansali, Shekhar</creator><general>AIP Publishing LLC</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20130501</creationdate><title>Microfluidic device for trapping and monitoring three dimensional multicell spheroids using electrical impedance spectroscopy</title><author>Luongo, Kevin ; Holton, Angela ; Kaushik, Ajeet ; Spence, Paige ; Ng, Beng ; Deschenes, Robert ; Sundaram, Shankar ; Bhansali, Shekhar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c511t-ab15ae105e671d68b2af5814b0f2009c8c9df65f26a339562865f181f76bac1e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Regular</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Luongo, Kevin</creatorcontrib><creatorcontrib>Holton, Angela</creatorcontrib><creatorcontrib>Kaushik, Ajeet</creatorcontrib><creatorcontrib>Spence, Paige</creatorcontrib><creatorcontrib>Ng, Beng</creatorcontrib><creatorcontrib>Deschenes, Robert</creatorcontrib><creatorcontrib>Sundaram, Shankar</creatorcontrib><creatorcontrib>Bhansali, Shekhar</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biomicrofluidics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Luongo, Kevin</au><au>Holton, Angela</au><au>Kaushik, Ajeet</au><au>Spence, Paige</au><au>Ng, Beng</au><au>Deschenes, Robert</au><au>Sundaram, Shankar</au><au>Bhansali, Shekhar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microfluidic device for trapping and monitoring three dimensional multicell spheroids using electrical impedance spectroscopy</atitle><jtitle>Biomicrofluidics</jtitle><addtitle>Biomicrofluidics</addtitle><date>2013-05-01</date><risdate>2013</risdate><volume>7</volume><issue>3</issue><spage>34108</spage><epage>34108</epage><pages>34108-34108</pages><issn>1932-1058</issn><eissn>1932-1058</eissn><coden>BIOMGB</coden><abstract>In this paper, we report the design, fabrication, and testing of a lab-on-a-chip based microfluidic device for application of trapping and measuring the dielectric properties of microtumors over time using electrical impedance spectroscopy (EIS). Microelectromechanical system (MEMS) techniques were used to embed opposing electrodes onto the top and bottom surfaces of a microfluidic channel fabricated using Pyrex substrate, chrome gold, SU-8, and polydimethylsiloxane. Differing concentrations of cell culture medium, differing sized polystyrene beads, and MCF-7 microtumor spheroids were used to validate the designs ability to detect background conductivity changes and dielectric particle diameter changes between electrodes. The observed changes in cell medium concentrations demonstrated a linear relation to extracted solution resistance (Rs), while polystyrene beads and multicell spheroids induced changes in magnitude consistent with diameter increase. This design permits optical correlation between electrical measurements and EIS spectra.</abstract><cop>United States</cop><pub>AIP Publishing LLC</pub><pmid>24404028</pmid><doi>10.1063/1.4809590</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1932-1058 |
ispartof | Biomicrofluidics, 2013-05, Vol.7 (3), p.34108-34108 |
issn | 1932-1058 1932-1058 |
language | eng |
recordid | cdi_crossref_primary_10_1063_1_4809590 |
source | AIP Journals Complete; EZB-FREE-00999 freely available EZB journals; PubMed Central |
subjects | Regular |
title | Microfluidic device for trapping and monitoring three dimensional multicell spheroids using electrical impedance spectroscopy |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T05%3A17%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Microfluidic%20device%20for%20trapping%20and%20monitoring%20three%20dimensional%20multicell%20spheroids%20using%20electrical%20impedance%20spectroscopy&rft.jtitle=Biomicrofluidics&rft.au=Luongo,%20Kevin&rft.date=2013-05-01&rft.volume=7&rft.issue=3&rft.spage=34108&rft.epage=34108&rft.pages=34108-34108&rft.issn=1932-1058&rft.eissn=1932-1058&rft.coden=BIOMGB&rft_id=info:doi/10.1063/1.4809590&rft_dat=%3Cproquest_cross%3E1490703916%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1490703916&rft_id=info:pmid/24404028&rfr_iscdi=true |