Direct full compensation of the aberrations in quantitative phase microscopy of thin objects by a single digital hologram

Aberrations and the distortions due to the imaging optics can be compensated in quantitative phase microscopy of thin phase objects by digital holography using a single hologram. The reconstructed quantitative phase microscopy phase distribution map can be directly corrected in the reconstructed ima...

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Veröffentlicht in:Applied physics letters 2007-01, Vol.90 (4), p.041104-041104-3
Hauptverfasser: Miccio, L., Alfieri, D., Grilli, S., Ferraro, P., Finizio, A., De Petrocellis, L., Nicola, S. D.
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container_issue 4
container_start_page 041104
container_title Applied physics letters
container_volume 90
creator Miccio, L.
Alfieri, D.
Grilli, S.
Ferraro, P.
Finizio, A.
De Petrocellis, L.
Nicola, S. D.
description Aberrations and the distortions due to the imaging optics can be compensated in quantitative phase microscopy of thin phase objects by digital holography using a single hologram. The reconstructed quantitative phase microscopy phase distribution map can be directly corrected in the reconstructed image plane by a numerical method. To remove this unwanted aberration, in the special case of thin objects, the authors perform a two-dimensional fit with the Zernike polynomials of the reconstructed unwrapped phase. Subtraction of the fitted polynomial from the original phase map gives quantitative phase microscopy phase map free of aberrations.
doi_str_mv 10.1063/1.2432287
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title Direct full compensation of the aberrations in quantitative phase microscopy of thin objects by a single digital hologram
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