Excitation of fluorescence decay using a 265nm pulsed light-emitting diode: Evidence for aqueous phenylalanine rotamers

Excitation of fluorescence decay using a 265nm pulsed light-emitting diode: Evidence for aqueous phenylalanine rotamers

The authors describe the characteristics and application of a 265nm AlGaN light-emitting diode (LED) operated at 1MHz repetition rate, 1.2ns pulse duration, 1.32μW average power, 2.3mW peak power, and ∼12nm bandwidth. The LED enables the fluorescence decay of weakly emitting phenylalanine to be meas...

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Veröffentlicht in:Applied physics letters 2006-08, Vol.89 (6)
Hauptverfasser: McGuinness, Colin D., Macmillan, Alexander M., Sagoo, Kulwinder, McLoskey, David, Birch, David J. S.
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container_issue 6
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container_title Applied physics letters
container_volume 89
creator McGuinness, Colin D.
Macmillan, Alexander M.
Sagoo, Kulwinder
McLoskey, David
Birch, David J. S.
description The authors describe the characteristics and application of a 265nm AlGaN light-emitting diode (LED) operated at 1MHz repetition rate, 1.2ns pulse duration, 1.32μW average power, 2.3mW peak power, and ∼12nm bandwidth. The LED enables the fluorescence decay of weakly emitting phenylalanine to be measured routinely, even in dilute solution. For pH of 6–9.2, the authors find evidence for a biexponential rather than monoexponential decay, providing direct evidence for the presence of phenylalanine rotamers with a photophysics closer to the other two fluorescent amino acids tryrosine and tryptophan than has previously been reported.
doi_str_mv 10.1063/1.2245441
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title Excitation of fluorescence decay using a 265nm pulsed light-emitting diode: Evidence for aqueous phenylalanine rotamers
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