Label-free probing of the binding state of DNA by time-domain terahertz sensing

Label-free probing of the binding state of DNA by time-domain terahertz sensing

We present a promising approach for the label-free characterization of genetic material. Time-resolved terahertz (THz) transmission analysis of polynucleotides demonstrate a strong dependence of the complex refractive index on the binding state (hybridized/denatured) of deoxyribonucleic acid (DNA) m...

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Veröffentlicht in:Applied physics letters 2000-12, Vol.77 (24), p.4049-4051
Hauptverfasser: Brucherseifer, M., Nagel, M., Haring Bolivar, P., Kurz, H., Bosserhoff, A., Büttner, R.
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container_end_page 4051
container_issue 24
container_start_page 4049
container_title Applied physics letters
container_volume 77
creator Brucherseifer, M.
Nagel, M.
Haring Bolivar, P.
Kurz, H.
Bosserhoff, A.
Büttner, R.
description We present a promising approach for the label-free characterization of genetic material. Time-resolved terahertz (THz) transmission analysis of polynucleotides demonstrate a strong dependence of the complex refractive index on the binding state (hybridized/denatured) of deoxyribonucleic acid (DNA) molecules. By monitoring THz transients, one can thus infer the binding state of oligo- and polynucleotides, and hence identify polynucleotides by detecting the binding of unknown polynucleotide DNA sequences to known probe molecules. A broadband experimental demonstration in a free-space configuration, as well as a discussion of the potential application for next generation gene chips is presented.
doi_str_mv 10.1063/1.1332415
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title Label-free probing of the binding state of DNA by time-domain terahertz sensing
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