Fatty Acid Synthetizing Enzymes in Adipocytes and Stromavascular Fraction of Hyperthyroid Rat Adipose Tissue
Summary In the rat, hyperthyroidism induced from birth has been shown to increase both adipose cell recruitment and de novo lipogenesis at 6 weeks of age. Since preadipose cells are included into the Stromavascular fraction (SVF) of adipose tissue, fatty acid synthetizing enzyme activities were esti...
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Veröffentlicht in: | Hormone and metabolic research 1989-10, Vol.21 (10), p.537-541 |
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description | Summary
In the rat, hyperthyroidism induced from birth has been shown to increase both adipose cell recruitment and de novo lipogenesis at 6 weeks of age. Since preadipose cells are included into the Stromavascular fraction (SVF) of adipose tissue, fatty acid synthetizing enzyme activities were estimated separately in adipocytes and SVF cells of adipose tissue of 6 week-old hyperthyroid rats. Fatty acid synthetase (FAS), malic enzyme (ME), glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) activities were generally increased in adipocytes and in SVF cells when compared to age-matched controls. Among the enzymes studied, ME activity displayed the highest stimulation in SVF cells and was much more stimulated in these cells than in adipocytes (214% and 73% increase, respectively, above control values, P < 0.001). These results show that, in vivo, SVF cells and adipocytes can respond differently to an hormonal stimulation and raise the question of the role of ME in the adipose conversion. |
doi_str_mv | 10.1055/s-2007-1009282 |
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In the rat, hyperthyroidism induced from birth has been shown to increase both adipose cell recruitment and de novo lipogenesis at 6 weeks of age. Since preadipose cells are included into the Stromavascular fraction (SVF) of adipose tissue, fatty acid synthetizing enzyme activities were estimated separately in adipocytes and SVF cells of adipose tissue of 6 week-old hyperthyroid rats. Fatty acid synthetase (FAS), malic enzyme (ME), glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) activities were generally increased in adipocytes and in SVF cells when compared to age-matched controls. Among the enzymes studied, ME activity displayed the highest stimulation in SVF cells and was much more stimulated in these cells than in adipocytes (214% and 73% increase, respectively, above control values, P < 0.001). These results show that, in vivo, SVF cells and adipocytes can respond differently to an hormonal stimulation and raise the question of the role of ME in the adipose conversion.</description><identifier>ISSN: 0018-5043</identifier><identifier>EISSN: 1439-4286</identifier><identifier>DOI: 10.1055/s-2007-1009282</identifier><identifier>PMID: 2807142</identifier><identifier>CODEN: HMMRA2</identifier><language>eng</language><publisher>Stuttgart: Thieme</publisher><subject>Adipose Tissue - cytology ; Adipose Tissue - enzymology ; Animals ; Biological and medical sciences ; Cell Count ; Endocrinopathies ; Fatty Acid Synthases - metabolism ; Fatty Acids - biosynthesis ; Glucosephosphate Dehydrogenase - metabolism ; Hyperthyroidism - chemically induced ; Hyperthyroidism - enzymology ; Malate Dehydrogenase - metabolism ; Male ; Medical sciences ; Non tumoral diseases. Target tissue resistance. Benign neoplasms ; Originals Basic ; Phosphogluconate Dehydrogenase - metabolism ; Rats ; Rats, Inbred Strains ; Thyroid. Thyroid axis (diseases) ; Thyroxine</subject><ispartof>Hormone and metabolic research, 1989-10, Vol.21 (10), p.537-541</ispartof><rights>Georg Thieme Verlag, Stuttgart · New York</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2732-eb9f427e5e41be1dd25876acb8df72aa6fafbb1d8a89434259bb19028ec96903</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.thieme-connect.de/products/ejournals/pdf/10.1055/s-2007-1009282.pdf$$EPDF$$P50$$Gthieme$$H</linktopdf><link.rule.ids>314,780,784,3015,3016,27923,27924,54558</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19675502$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2807142$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Levacher, C.</creatorcontrib><creatorcontrib>Picon, L.</creatorcontrib><title>Fatty Acid Synthetizing Enzymes in Adipocytes and Stromavascular Fraction of Hyperthyroid Rat Adipose Tissue</title><title>Hormone and metabolic research</title><addtitle>Horm Metab Res</addtitle><description>Summary
In the rat, hyperthyroidism induced from birth has been shown to increase both adipose cell recruitment and de novo lipogenesis at 6 weeks of age. Since preadipose cells are included into the Stromavascular fraction (SVF) of adipose tissue, fatty acid synthetizing enzyme activities were estimated separately in adipocytes and SVF cells of adipose tissue of 6 week-old hyperthyroid rats. Fatty acid synthetase (FAS), malic enzyme (ME), glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) activities were generally increased in adipocytes and in SVF cells when compared to age-matched controls. Among the enzymes studied, ME activity displayed the highest stimulation in SVF cells and was much more stimulated in these cells than in adipocytes (214% and 73% increase, respectively, above control values, P < 0.001). These results show that, in vivo, SVF cells and adipocytes can respond differently to an hormonal stimulation and raise the question of the role of ME in the adipose conversion.</description><subject>Adipose Tissue - cytology</subject><subject>Adipose Tissue - enzymology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Count</subject><subject>Endocrinopathies</subject><subject>Fatty Acid Synthases - metabolism</subject><subject>Fatty Acids - biosynthesis</subject><subject>Glucosephosphate Dehydrogenase - metabolism</subject><subject>Hyperthyroidism - chemically induced</subject><subject>Hyperthyroidism - enzymology</subject><subject>Malate Dehydrogenase - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Non tumoral diseases. Target tissue resistance. Benign neoplasms</subject><subject>Originals Basic</subject><subject>Phosphogluconate Dehydrogenase - metabolism</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Thyroid. Thyroid axis (diseases)</subject><subject>Thyroxine</subject><issn>0018-5043</issn><issn>1439-4286</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1UM1LwzAcDaLMOb16E3LxmJmkaZscx9icMBB095KmqcvoF0kqdH-9GS168vTj8T5-vAfAI8FLguP4xSGKcYoIxoJyegXmhEUCMcqTazDHmHAUYxbdgjvnTgEyQdgMzCjHKWF0Dqqt9H6AK2UK-Dk0_qi9OZvmC26a81BrB00DV4XpWjX4gGQTZN62tfyWTvWVtHBrpfKmbWBbwt3QaeuPg21D3If0o9VpeDDO9foe3JSycvphugtw2G4O6x3av7--rVd7pGgaUaRzUTKa6lgzkmtSFDTmaSJVzosypVImpSzznBRccsEiRmMRkMCUayUSgaMFWI6xyrbOWV1mnTW1tENGcHYZLXPZZbRsGi0YnkZD1-e1Ln7l00qBf574UFpWpZWNMu4vVSRpHOOLDo06fzS61tmp7W0Tiv739we7eoQU</recordid><startdate>198910</startdate><enddate>198910</enddate><creator>Levacher, C.</creator><creator>Picon, L.</creator><general>Thieme</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>198910</creationdate><title>Fatty Acid Synthetizing Enzymes in Adipocytes and Stromavascular Fraction of Hyperthyroid Rat Adipose Tissue</title><author>Levacher, C. ; Picon, L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2732-eb9f427e5e41be1dd25876acb8df72aa6fafbb1d8a89434259bb19028ec96903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Adipose Tissue - cytology</topic><topic>Adipose Tissue - enzymology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Count</topic><topic>Endocrinopathies</topic><topic>Fatty Acid Synthases - metabolism</topic><topic>Fatty Acids - biosynthesis</topic><topic>Glucosephosphate Dehydrogenase - metabolism</topic><topic>Hyperthyroidism - chemically induced</topic><topic>Hyperthyroidism - enzymology</topic><topic>Malate Dehydrogenase - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Non tumoral diseases. Target tissue resistance. Benign neoplasms</topic><topic>Originals Basic</topic><topic>Phosphogluconate Dehydrogenase - metabolism</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Thyroid. Thyroid axis (diseases)</topic><topic>Thyroxine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Levacher, C.</creatorcontrib><creatorcontrib>Picon, L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Hormone and metabolic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Levacher, C.</au><au>Picon, L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fatty Acid Synthetizing Enzymes in Adipocytes and Stromavascular Fraction of Hyperthyroid Rat Adipose Tissue</atitle><jtitle>Hormone and metabolic research</jtitle><addtitle>Horm Metab Res</addtitle><date>1989-10</date><risdate>1989</risdate><volume>21</volume><issue>10</issue><spage>537</spage><epage>541</epage><pages>537-541</pages><issn>0018-5043</issn><eissn>1439-4286</eissn><coden>HMMRA2</coden><abstract>Summary
In the rat, hyperthyroidism induced from birth has been shown to increase both adipose cell recruitment and de novo lipogenesis at 6 weeks of age. Since preadipose cells are included into the Stromavascular fraction (SVF) of adipose tissue, fatty acid synthetizing enzyme activities were estimated separately in adipocytes and SVF cells of adipose tissue of 6 week-old hyperthyroid rats. Fatty acid synthetase (FAS), malic enzyme (ME), glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) activities were generally increased in adipocytes and in SVF cells when compared to age-matched controls. Among the enzymes studied, ME activity displayed the highest stimulation in SVF cells and was much more stimulated in these cells than in adipocytes (214% and 73% increase, respectively, above control values, P < 0.001). These results show that, in vivo, SVF cells and adipocytes can respond differently to an hormonal stimulation and raise the question of the role of ME in the adipose conversion.</abstract><cop>Stuttgart</cop><cop>New York, NY</cop><pub>Thieme</pub><pmid>2807142</pmid><doi>10.1055/s-2007-1009282</doi><tpages>5</tpages></addata></record> |
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subjects | Adipose Tissue - cytology Adipose Tissue - enzymology Animals Biological and medical sciences Cell Count Endocrinopathies Fatty Acid Synthases - metabolism Fatty Acids - biosynthesis Glucosephosphate Dehydrogenase - metabolism Hyperthyroidism - chemically induced Hyperthyroidism - enzymology Malate Dehydrogenase - metabolism Male Medical sciences Non tumoral diseases. Target tissue resistance. Benign neoplasms Originals Basic Phosphogluconate Dehydrogenase - metabolism Rats Rats, Inbred Strains Thyroid. Thyroid axis (diseases) Thyroxine |
title | Fatty Acid Synthetizing Enzymes in Adipocytes and Stromavascular Fraction of Hyperthyroid Rat Adipose Tissue |
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