Fatty Acid Synthetizing Enzymes in Adipocytes and Stromavascular Fraction of Hyperthyroid Rat Adipose Tissue

Summary In the rat, hyperthyroidism induced from birth has been shown to increase both adipose cell recruitment and de novo lipogenesis at 6 weeks of age. Since preadipose cells are included into the Stromavascular fraction (SVF) of adipose tissue, fatty acid synthetizing enzyme activities were esti...

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Veröffentlicht in:Hormone and metabolic research 1989-10, Vol.21 (10), p.537-541
Hauptverfasser: Levacher, C., Picon, L.
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creator Levacher, C.
Picon, L.
description Summary In the rat, hyperthyroidism induced from birth has been shown to increase both adipose cell recruitment and de novo lipogenesis at 6 weeks of age. Since preadipose cells are included into the Stromavascular fraction (SVF) of adipose tissue, fatty acid synthetizing enzyme activities were estimated separately in adipocytes and SVF cells of adipose tissue of 6 week-old hyperthyroid rats. Fatty acid synthetase (FAS), malic enzyme (ME), glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) activities were generally increased in adipocytes and in SVF cells when compared to age-matched controls. Among the enzymes studied, ME activity displayed the highest stimulation in SVF cells and was much more stimulated in these cells than in adipocytes (214% and 73% increase, respectively, above control values, P < 0.001). These results show that, in vivo, SVF cells and adipocytes can respond differently to an hormonal stimulation and raise the question of the role of ME in the adipose conversion.
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Since preadipose cells are included into the Stromavascular fraction (SVF) of adipose tissue, fatty acid synthetizing enzyme activities were estimated separately in adipocytes and SVF cells of adipose tissue of 6 week-old hyperthyroid rats. Fatty acid synthetase (FAS), malic enzyme (ME), glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) activities were generally increased in adipocytes and in SVF cells when compared to age-matched controls. Among the enzymes studied, ME activity displayed the highest stimulation in SVF cells and was much more stimulated in these cells than in adipocytes (214% and 73% increase, respectively, above control values, P &lt; 0.001). These results show that, in vivo, SVF cells and adipocytes can respond differently to an hormonal stimulation and raise the question of the role of ME in the adipose conversion.</description><subject>Adipose Tissue - cytology</subject><subject>Adipose Tissue - enzymology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Count</subject><subject>Endocrinopathies</subject><subject>Fatty Acid Synthases - metabolism</subject><subject>Fatty Acids - biosynthesis</subject><subject>Glucosephosphate Dehydrogenase - metabolism</subject><subject>Hyperthyroidism - chemically induced</subject><subject>Hyperthyroidism - enzymology</subject><subject>Malate Dehydrogenase - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Non tumoral diseases. Target tissue resistance. Benign neoplasms</subject><subject>Originals Basic</subject><subject>Phosphogluconate Dehydrogenase - metabolism</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Thyroid. 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Target tissue resistance. Benign neoplasms</topic><topic>Originals Basic</topic><topic>Phosphogluconate Dehydrogenase - metabolism</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Thyroid. 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Since preadipose cells are included into the Stromavascular fraction (SVF) of adipose tissue, fatty acid synthetizing enzyme activities were estimated separately in adipocytes and SVF cells of adipose tissue of 6 week-old hyperthyroid rats. Fatty acid synthetase (FAS), malic enzyme (ME), glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) activities were generally increased in adipocytes and in SVF cells when compared to age-matched controls. Among the enzymes studied, ME activity displayed the highest stimulation in SVF cells and was much more stimulated in these cells than in adipocytes (214% and 73% increase, respectively, above control values, P &lt; 0.001). These results show that, in vivo, SVF cells and adipocytes can respond differently to an hormonal stimulation and raise the question of the role of ME in the adipose conversion.</abstract><cop>Stuttgart</cop><cop>New York, NY</cop><pub>Thieme</pub><pmid>2807142</pmid><doi>10.1055/s-2007-1009282</doi><tpages>5</tpages></addata></record>
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subjects Adipose Tissue - cytology
Adipose Tissue - enzymology
Animals
Biological and medical sciences
Cell Count
Endocrinopathies
Fatty Acid Synthases - metabolism
Fatty Acids - biosynthesis
Glucosephosphate Dehydrogenase - metabolism
Hyperthyroidism - chemically induced
Hyperthyroidism - enzymology
Malate Dehydrogenase - metabolism
Male
Medical sciences
Non tumoral diseases. Target tissue resistance. Benign neoplasms
Originals Basic
Phosphogluconate Dehydrogenase - metabolism
Rats
Rats, Inbred Strains
Thyroid. Thyroid axis (diseases)
Thyroxine
title Fatty Acid Synthetizing Enzymes in Adipocytes and Stromavascular Fraction of Hyperthyroid Rat Adipose Tissue
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